About: A comparison of non-magnetic and magnetic beads for measuring IgG antibodies against P. vivax antigens in a multiplexed bead-based assay using Luminex® technology (Bio-Plex® 200 or MAGPIX®)

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About: A comparison of non-magnetic and magnetic beads for measuring IgG antibodies against P. vivax antigens in a multiplexed bead-based assay using Luminex® technology (Bio-Plex® 200 or MAGPIX®) Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A comparison of non-magnetic and magnetic beads for measuring IgG antibodies against P. vivax antigens in a multiplexed bead-based assay using Luminex® technology (Bio-Plex® 200 or MAGPIX®)
Creator	King, Christopher Tham, Wai-Hong Tsuboi, Takafumi Healer, Julie Bourke, Caitlin Brewster, Jessica Chitnis, Chetan Fong, Rich Harbers, Matthias Kazura, James Liu, Zoe Longley, Rhea Mazhari, Ramin Mueller, Ome-7 Kaius, Maria Robinson, Leanne Sattabongkot, Jetsumon Takashima, Eizo
Source	BioRxiv
abstract	Multiplexed bead-based assays that use Luminex xMAP® technology have become popular for measuring antibodies against proteins of interest in many fields, including malaria and more recently SARS-CoV-2/COVID-19. There are currently two formats that are widely used: non-magnetic beads or magnetic beads. Data is lacking regarding the comparability of results obtained using these two types of beads, and for assays run on different instruments. Whilst non-magnetic beads can only be run on flow-based instruments (such as the Luminex® 100/200™ or Bio-Plex® 200), magnetic beads can be run on both these and the newer MAGPIX® instruments. In this study we utilized a panel of purified recombinant Plasmodium vivax proteins and samples from malaria-endemic areas to measure P. vivax-specific IgG responses using different combinations of beads and instruments. We directly compared: i) non-magnetic versus magnetic beads run on a Bio-Plex® 200, ii) magnetic beads run on the Bio-Plex® 200 versus MAGPIX® and iii) non-magnetic beads run on a Bio-Plex® 200 versus magnetic beads run on the MAGPIX®. We also performed an external validation of our optimized assay. We observed that IgG antibody responses, measured against our panel of P. vivax proteins, were strongly correlated in all three of our comparisons, however higher amounts of protein were required for coupling to magnetic beads. Our external validation indicated that results generated in different laboratories using the same coupled beads are also highly comparable, particularly if a reference standard curve is used.
has issue date	2020-08-10(xsd:dateTime)
bibo:doi	10.1101/2020.08.10.243980
has license	biorxiv
sha1sum (hex)	74a86180a3af344d3fbcc5276906345110117a00
schema:url	https://doi.org/10.1101/2020.08.10.243980
resource representing a document's title	A comparison of non-magnetic and magnetic beads for measuring IgG antibodies against P. vivax antigens in a multiplexed bead-based assay using Luminex® technology (Bio-Plex® 200 or MAGPIX®)
schema:publication	bioRxiv
resource representing a document's body	covid:74a86180a3af344d3fbcc5276906345110117a00#body_text
is schema:about of	named entity 'P. vivax' named entity 'IgG' named entity 'measuring' named entity 'performed' named entity 'Plasmodium' named entity 'validation' named entity 'magnetic' named entity 'coupled' named entity 'laboratories' named entity 'COUPLED' named entity 'OBTAINED' named entity 'NON-MAGNETIC' named entity 'PLASMODIUM VIVAX' named entity 'OBSERVED' named entity 'INCLUDING' named entity 'DIRECTLY' named entity 'beads' named entity 'specific' named entity 'non-magnetic' named entity 'newer' named entity 'external' named entity 'beads' named entity 'measure' named entity 'coupling' named entity 'beads' named entity 'observed' named entity 'popular' named entity 'combinations' named entity 'malaria' named entity 'endemic areas' named entity 'Plasmodium vivax' named entity 'fields' named entity 'monobasic' named entity 'antigens' named entity 'antigen' named entity 'Bio-Rad' named entity 'multiplexed' named entity 'BSA' named entity 'protein' named entity 'serial dilution' named entity 'normal distribution' named entity 'protein' named entity 'P. vivax' named entity 'protein' named entity 'sodium phosphate' named entity 'protein' named entity 'standard curve' named entity 'protein' named entity 'P. vivax' named entity 'P. vivax' named entity 'antigen' named entity 'PBS' named entity 'standard curve' named entity 'assay' named entity 'scatter plots' named entity 'protein' named entity 'plasma pool' named entity 'PNG' named entity 'carboxylated' named entity 'scatter plots' named entity 'standard curve' named entity 'centrifugation' named entity '1/50' named entity 'plasma pool' named entity 'Plasmodium' named entity 'standard curve' named entity 'cross-link'

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