About: Development of a Novel Ex-vivo 3D Model to Screen Amoebicidal Activity on Infected Tissue

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Development of a Novel Ex-vivo 3D Model to Screen Amoebicidal Activity on Infected Tissue Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of a Novel Ex-vivo 3D Model to Screen Amoebicidal Activity on Infected Tissue
Creator	Carranza-Rosales, Pilar García-Davis, Sara Morán-Martínez, Javier Rivera, Gildardo Carranza-Torres, Irma De Graaf, I Denys Betancourt-Martínez, Nadia Groothuis, G Guzmán-Delgado, Nancy
topic	covid:74bedf868bf72275e1d5a60150246aaae93d3be5#this
Source	PMC
abstract	Amoebiasis is a parasitic disease that causes thousands of deaths every year, its adverse effects and resistance to conventional treatments have led to the search of new treatment options, as well as the development of novel screening methods. In this work, we implemented a 3D model of intestine and liver slices from hamsters that were infected ex vivo with virulent E. histolytica trophozoites. Results show preserved histology in both uninfected tissues as well as ulcerations, destruction of the epithelial cells, and inflammatory reaction in intestine slices and formation of micro abscesses, and the presence of amoebae in the sinusoidal spaces and in the interior of central veins in liver slices. The three chemically synthetized compounds T-001, T-011, and T-016, which act as amoebicides in vitro, were active in both infected tissues, as they decreased the number of trophozoites, and provoked death by disintegration of the amoeba, similar to metronidazole. However, compound T-011 induced signs of cytotoxicity to liver slices. Our results suggest that ex vivo cultures of precision-cut intestinal and liver slices represent a reliable 3D approach to evaluate novel amoebicidal compounds, and to simultaneously detect their toxicity, while reducing the number of experimental animals commonly required by other model systems.
has issue date	2019-06-10(xsd:dateTime)
bibo:doi	10.1038/s41598-019-44899-5
bibo:pmid	31182753
has license	cc-by
sha1sum (hex)	74bedf868bf72275e1d5a60150246aaae93d3be5
schema:url	https://doi.org/10.1038/s41598-019-44899-5
resource representing a document's title	Development of a Novel Ex-vivo 3D Model to Screen Amoebicidal Activity on Infected Tissue
has PubMed Central identifier	PMC6557822
has PubMed identifier	31182753
schema:publication	Sci Rep
resource representing a document's body	covid:74bedf868bf72275e1d5a60150246aaae93d3be5#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/74bedf868bf72275e1d5a60150246aaae93d3be5
is schema:about of	named entity 'DEVELOPMENT' named entity 'slices' named entity 'intestine' named entity 'NOVEL' named entity 'INFECTED' named entity 'MODEL OF' named entity 'E. HISTOLYTICA' named entity 'IMPLEMENTED' named entity 'ITS' named entity 'CAUSES' named entity 'ADVERSE EFFECTS' named entity 'VIRULENT' named entity 'WORK' named entity 'EVERY YEAR' named entity 'AMOEBIASIS' named entity 'LIVER' named entity 'SEARCH' named entity 'TROPHOZOITES' named entity 'THOUSANDS' named entity 'TISSUE' named entity 'TREATMENTS' named entity 'IS A' named entity 'NEW' named entity 'VIVO' named entity 'METHODS' named entity 'ACTIVITY' named entity 'SCREEN' named entity 'INFECTED' named entity 'LED' named entity 'HAVE' named entity 'SCREENING' named entity 'EX VIVO' named entity 'RESISTANCE TO' named entity 'TREATMENT OPTIONS' named entity '3D MODEL' named entity 'CONVENTIONAL' named entity 'PARASITIC DISEASE' named entity 'HAMSTERS' named entity '3D MODEL' named entity 'DEATHS' named entity 'DEVELOPMENT' named entity 'INTESTINE' named entity 'NOVEL' covid:arg/74bedf868bf72275e1d5a60150246aaae93d3be5 named entity 'screening' named entity 'resistance' named entity 'led' named entity 'E. histolytica' named entity 'human disease' named entity 'E. histolytica' named entity 'tissue viability' named entity 'metabolic transformation' named entity 'infection' named entity 'liver' named entity 'agarose' named entity 'amoebae' named entity 'metronidazole' named entity 'toxicological' named entity 'carbogen' named entity 'nature.com' named entity 'parasite' named entity 'intestine' named entity 'formalin' named entity 'biochemical' named entity 'nature.com' named entity 'anti-amoebic' named entity 'virulence' named entity 'virulent strain' named entity 'anesthesia' named entity 'liver' named entity 'morphological'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software