About: Capillary Electrophoresis of PCR fragments with 5´-labelled primers for testing the SARS-Cov-2

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About: Capillary Electrophoresis of PCR fragments with 5´-labelled primers for testing the SARS-Cov-2 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Capillary Electrophoresis of PCR fragments with 5´-labelled primers for testing the SARS-Cov-2
Creator	Melón, Santiago Rojo-Alba, Susana Alvarez, Victoria Alvarez-Argüelles, Marta Boga, José Castello-Abietar, Cristian Coto, Eliecer Cuesta-Llavona, Elías Gómez, Juan Leal-Negredo, Alvaro Ja, Boga Me, Alvarez-Argüelles
source	Elsevier; Medline; PMC
abstract	Due to the huge demand for SARS-Cov-2 determination,alternatives to the standard qtPCRtestsare potentially useful for increasing the number of samples screened. Our aim was to develop a direct fluorescent PCR capillary-electrophoresis detection of the viral genome. We validated this approach on several SARS-Cov-2 positive and negative samples.We isolated the naso-pharingealRNA from 20 positive and 10 negative samples. The cDNA was synthesised and two fragments of the SARS-Cov-2 were amplified. One of the primers for each pair was 5´-end fluorochrome labelled. The amplifications were subjected to capillary electrophoresis in ABI3130 sequencers to visualize the fluorescent peaks.The two SARS-Cov-2 fragments were successfully amplified in the positive samples, while the negative samples did not render fluorescent peaks. In conclusion, we describe and alternative method to identify the SARS-Cov-2 genome that could be scaled to the analysis of approximately 100 samples in less than 5 hours. By combining a standard PCR with capillary electrophoresis our approach would overcome the limits imposed to many labs by the qtPCR and increase the testing capacity.
has issue date	2020-07-10(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2020.113937
bibo:pmid	32659241
has license	no-cc
sha1sum (hex)	74c964d18dcebf17812d4fc2d586f96dc8d5cefb
schema:url	https://doi.org/10.1016/j.jviromet.2020.113937
resource representing a document's title	Capillary Electrophoresis of PCR fragments with 5´-labelled primers for testing the SARS-Cov-2
has PubMed Central identifier	PMC7351060
has PubMed identifier	32659241
schema:publication	J Virol Methods
resource representing a document's body	covid:74c964d18dcebf17812d4fc2d586f96dc8d5cefb#body_text
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