About: Structure-based Design of Prefusion-stabilized SARS-CoV-2 Spikes

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Structure-based Design of Prefusion-stabilized SARS-CoV-2 Spikes
Creator	Wang, Nianshuang Wrapp, Daniel Hsieh, Ching-Lin Mclellan, Jason Maynard, Jennifer Goldsmith, Jory Amengor, Dzifa Byrne, Patrick Chou, Chia-Wei Divenere, Andrea Finkelstein, Ilya Hjorth, Christy Javanmardi, Kamyab Johnson, Nicole Kuo, Hung-Che Le, Kevin Lee, Gene-Wei Liu, Yutong Ludes-Meyers, John Nguyen, Annalee Park, Juyeon Schaub, Jeffrey
source	BioRxiv; Medline; PMC; WHO
abstract	The COVID-19 pandemic caused by the novel coronavirus SARS-CoV-2 has led to accelerated efforts to develop therapeutics, diagnostics, and vaccines to mitigate this public health emergency. A key target of these efforts is the spike (S) protein, a large trimeric class I fusion protein that is metastable and difficult to produce recombinantly in large quantities. Here, we designed and expressed over 100 structure-guided spike variants based upon a previously determined cryo-EM structure of the prefusion SARS-CoV-2 spike. Biochemical, biophysical and structural characterization of these variants identified numerous individual substitutions that increased protein yields and stability. The best variant, HexaPro, has six beneficial proline substitutions leading to ~10-fold higher expression than its parental construct and is able to withstand heat stress, storage at room temperature, and multiple freeze-thaws. A 3.2 Å-resolution cryo-EM structure of HexaPro confirmed that it retains the prefusion spike conformation. High-yield production of a stabilized prefusion spike protein will accelerate the development of vaccines and serological diagnostics for SARS-CoV-2.
has issue date	2020-05-30(xsd:dateTime)
bibo:doi	10.1101/2020.05.30.125484
bibo:pmid	32577660
has license	cc-by-nc-nd
sha1sum (hex)	7552442660db5faf25d03adbeb418f486f75e9a6
schema:url	https://doi.org/10.1101/2020.05.30.125484
resource representing a document's title	Structure-based Design of Prefusion-stabilized SARS-CoV-2 Spikes
has PubMed Central identifier	PMC7302215
has PubMed identifier	32577660
schema:publication	bioRxiv
resource representing a document's body	covid:7552442660db5faf25d03adbeb418f486f75e9a6#body_text
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