About: Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification
Creator	Zhang, Jie Liu, Xiang-Tao Sun, De-Hui Liu, Qing Cai, Xue-Peng Chen, Hao-Tai Chu, Yue-Feng Liu, Yong-Sheng Luo, Xue-Nong
Source	Elsevier; Medline; PMC
abstract	A method of loop-mediated isothermal amplification (LAMP) was employed to develop a rapid and simple detection system for porcine circovirus type 2 (PCV2). The amplification could be finished in 60 min under isothermal condition at 64 °C by employing a set of four primers targeting the cap gene of PCV2. The LAMP assay showed higher sensitivity than the conventional PCR, with a detection limit of five copies per tube of purified PCV2 genomic DNA. No cross-reactivity was observed from the samples of other related viruses including porcine circovirus type 1 (PCV1), porcine parvovirus (PPV), porcine pseudorabies virus (PRV) and porcine reproductive and respiratory syndrome virus (PRRSV). The detection rate of PCV2 LAMP for 86 clinical samples was 96.5% and appeared greater than that of the PCR method. The LAMP assay reported can provide a rapid yet simple test of PCV2 suitable for laboratory diagnosis and pen-side detection due to ease of operation and the requirement of only a regular water bath or heat block for the reaction.
has issue date	2008-03-19(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2008.01.023
bibo:pmid	18355932
has license	no-cc
sha1sum (hex)	75da2b43b5d294fe2ba1bb8f8a17620f20aaea4f
schema:url	https://doi.org/10.1016/j.jviromet.2008.01.023
resource representing a document's title	Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification
has PubMed Central identifier	PMC7112855
has PubMed identifier	18355932
schema:publication	J Virol Methods
resource representing a document's body	covid:75da2b43b5d294fe2ba1bb8f8a17620f20aaea4f#body_text
is schema:about of	named entity 'virus' named entity 'simple' named entity 'detection' named entity 'condition' named entity 'cross-reactivity' named entity 'LAMP' named entity 'porcine circovirus' named entity 'RAPID' named entity 'PORCINE' named entity 'OPERATION' named entity 'GENOMIC DNA' named entity 'PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS' named entity 'BLOCK' named entity 'PORCINE PARVOVIRUS' named entity 'LAMP' named entity 'EASE' named entity 'PEN' named entity 'CONDITION' named entity 'REACTIVITY' named entity 'SIMPLE' named entity 'REQUIREMENT' named entity 'SIMPLE TEST' named entity 'SUITABLE' named entity 'DETECTION LIMIT' named entity 'HIGHER' named entity 'DETECTION' named entity 'PRIMERS' named entity 'COPIES' named entity 'INCLUDING' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'DETECTION' named entity 'PORCINE CIRCOVIRUS TYPE 2' named entity 'PORCINE CIRCOVIRUS TYPE 1' named entity 'COULD BE' named entity 'RATE' named entity 'GREATER THAN' named entity 'CROSS' named entity 'WATER BATH' named entity 'SET' named entity 'LABORATORY DIAGNOSIS' named entity 'REGULAR' named entity 'OBSERVED' named entity 'VIRUSES' named entity 'FINISHED' named entity 'PROVIDE' named entity 'RAPID' named entity 'GENE' named entity 'SENSITIVITY' named entity 'REACTION' named entity 'REPORTED' named entity 'PSEUDORABIES VIRUS' named entity 'MIN' named entity 'CAP' named entity 'SYSTEM' named entity 'ASSAY' named entity 'TUBE' named entity 'SAMPLES' named entity 'CONVENTIONAL' named entity 'PCR' named entity 'CLINICAL' named entity 'EMPLOYED' named entity 'RELATED' named entity 'METHOD' named entity 'HEAT' named entity 'TARGETING' named entity 'DUE TO' named entity 'PORCINE CIRCOVIRUS TYPE 2' named entity 'PURIFIED' named entity '96.5' named entity '80%' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'AMPLIFICATION' covid:arg/75da2b43b5d294fe2ba1bb8f8a17620f20aaea4f

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software