About: Functional plasticity of antibacterial EndoU toxins

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About: Functional plasticity of antibacterial EndoU toxins Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Functional plasticity of antibacterial EndoU toxins
Creator	Joachimiak, Andrzej Koskiniemi, Sanna Michalska, Karolina Hayes, Christopher Eschenfeldt, William Goulding, Celia Stols, Lucy Willett, Julia Dinh, ‡ Jones, Allison Low, David Nguyen, Josephine Nhan, Quan
Source	Medline; PMC
abstract	Bacteria use several different secretion systems to deliver toxic EndoU ribonucleases into neighboring cells. Here, we present the first structure of a prokaryotic EndoU toxin in complex with its cognate immunity protein. The contact-dependent growth inhibition toxin CdiA-CT(STECO31) from Escherichia coli STEC_O31 adopts the eukaryotic EndoU fold and shares greatest structural homology with the nuclease domain of coronavirus Nsp15. The toxin contains a canonical His-His-Lys catalytic triad in the same arrangement as eukaryotic EndoU domains, but lacks the uridylate-specific ribonuclease activity that characterizes the superfamily. Comparative sequence analysis indicates that bacterial EndoU domains segregate into at least three major clades based on structural variations in the N-terminal subdomain. Representative EndoU nucleases from clades I and II degrade tRNA molecules with little specificity. In contrast, CdiA-CT(STECO31) and other clade III toxins are specific anticodon nucleases that cleave tRNA(Glu) between nucleotides C37 and m(2)A38. These findings suggest that the EndoU fold is a versatile scaffold for the evolution of novel substrate specificities. Such functional plasticity may account for the widespread use of EndoU effectors by diverse inter-bacterial toxin delivery systems.
has issue date	2018-08-01(xsd:dateTime)
bibo:doi	10.1111/mmi.14007
bibo:pmid	29923643
has license	bronze-oa
sha1sum (hex)	776c56df617a70429e9a2a23b8926ed854af43d0
schema:url	https://doi.org/10.1111/mmi.14007
resource representing a document's title	Functional plasticity of antibacterial EndoU toxins
has PubMed Central identifier	PMC6173971
has PubMed identifier	29923643
schema:publication	Molecular Microbiology
resource representing a document's body	covid:776c56df617a70429e9a2a23b8926ed854af43d0#body_text
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