About: Establishment of a Porcine Parvovirus (PPV) LAMP Visual Rapid Detection Method

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Establishment of a Porcine Parvovirus (PPV) LAMP Visual Rapid Detection Method
Creator	Zhang, Qi Xu, Yan Zhao, Kai Du, Yanan Li, Chunhua Li, Zhang He, Xizhong Hu, Ruili Liang, Jieling Ni, Jianping Zhao, Binan Liang, Ni Xu, Du
source	Elsevier; Medline; PMC
abstract	Porcine parvovirus (PPV) is one of the major causes of reproductive pig disease. Due to its serious nature, wide spread and consequent great damage to the swine industry, an effective, rapid and convenient method for its detection is needed. A loop-mediated isothermal amplification (LAMP) assay was established to detect PPV infection. Two pairs of primers were specifically designed to recognize the six different sequences of open reading frame1 (ORF1) gene. The optimized LAMP program was as follows: 50 min at 59 °C followed by 3 min at 80 °C.The amplified products were analyzed both by visual inspection after staining with SYBR Green I dye and by conventional agarose gel electrophoresis. Both methods showed the same sensitivity. The limit of detection (LOD) for PPV by LAMP was 10 copies, which is 100-fold lower than conventional PCR. Our LAMP assay did not cross-react with other viruses. We used the established LAMP system to test 1100 field samples and detected 660 positives. The LAMP detection method for PPV represents a visual, sensitive and rapid assay which can detect the virus in the field, offering an attractive alternative for the PPV detection methods currently in use.
has issue date	2020-07-01(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2020.113924
bibo:pmid	32621958
has license	no-cc
sha1sum (hex)	789a0e42916a7a3def358f13234df2c6ebeb8c0e
schema:url	https://doi.org/10.1016/j.jviromet.2020.113924
resource representing a document's title	Establishment of a Porcine Parvovirus (PPV) LAMP Visual Rapid Detection Method
has PubMed Central identifier	PMC7328634
has PubMed identifier	32621958
schema:publication	J Virol Methods
resource representing a document's body	covid:789a0e42916a7a3def358f13234df2c6ebeb8c0e#body_text
is schema:about of	named entity 'wide' named entity 'PPV' named entity 'damage' named entity 'rapid' named entity 'PPV' named entity 'test' named entity 'major' named entity 'Parvovirus' named entity 'Establishment' named entity 'DUE TO' named entity 'EFFECTIVE' named entity 'REPRODUCTIVE' named entity 'ATTRACTIVE' named entity 'INDUSTRY' named entity 'LOWER' named entity 'FOLLOWS' named entity 'PPV' named entity 'CONVENTIONAL' named entity 'USE' named entity 'CAUSES' named entity 'LAMP' named entity 'DETECT' named entity 'NEEDED' named entity 'pairs' named entity 'sensitive' named entity 'swine' named entity 'min' named entity 'SYBR Green I' named entity 'PPV' named entity 'optimized' named entity 'conventional' named entity 'LAMP' named entity 'conventional' named entity 'loop-mediated isothermal amplification' named entity 'alternative' named entity 'Porcine parvovirus' named entity 'cross-react' named entity 'primers' named entity 'PCR' named entity 'limit of detection' named entity 'ORF1' named entity 'assay' named entity 'Porcine Parvovirus' named entity 'sequenced' named entity 'pig' named entity 'serum samples' named entity 'serum' named entity 'Enzyme linked immunosorbent assay' named entity 'pig' named entity 'gene' named entity 'PCR' named entity 'PCR' named entity 'PCR' named entity 'primers' named entity 'infection' named entity 'agarose gel electrophoresis' named entity 'pipette' named entity 'genomic DNA' named entity 'SYBR Green' named entity 'PCR' named entity 'infection' named entity 'RNA' named entity 'nucleic acids' named entity 'PCR' named entity 'detection limit' named entity 'genome' named entity 'blood samples' named entity 'clinical symptoms' named entity 'porcine parvovirus'

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