About: The immunity induced by recombinant spike proteins of SARS coronavirus in Balb/c mice

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About: The immunity induced by recombinant spike proteins of SARS coronavirus in Balb/c mice Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The immunity induced by recombinant spike proteins of SARS coronavirus in Balb/c mice
Creator	Li, Qin Huang, Dana Lei, Mingjun Qiping, Lin Shishan, Yuan Wang, Ximing Wu, Shaoting
Source	Medline; PMC
abstract	The immune effect of two recombinant protein fragments of spike protein in severe acute respiratory syndrome coronavirus (SARS CoV) was investigated in Balb/c mice. Two partial spike gene fragments S1 (322 1464 bp) and S2 (2170 2814 bp) of SARS coronavirus were amplified by RT-PCR, and cloned into pET-23a prokaryotic expression vector, then transformed into competent Escherichia E. coli BL21 (DE3)(pLysS) respectively. Recombinant proteins were expressed and purified by Ni(2+) immobilized metal ion affinity chromatography. The purified proteins mixed with complete Freund adjuvant were injected into Balb/c mice three times at a two-week interval. High titer antibody was detected in the serum of immunized Balb/c mice, and mice immunized with S1 protein produced high titer IgG1, IgG2a, IgG2b and IgG3, while those immunized with S2 protein produced high titer IgG1, IgG2a, but lower titer IgG2b and IgG3. Serum IFN-concentration was increased significantly but the concentrations of Il-2, IL-4 and IL-10 had no significant change. And a marked increase was observed in the number of spleen CD8+ T cells. The results showed that recombinant proteins of SARS coronavirus spike protein induced hormonal and cellular immune response in Balb/c mice.
has issue date	2007-01-01(xsd:dateTime)
bibo:doi	10.1007/s11596-007-0301-0
bibo:pmid	17641827
has license	no-cc
sha1sum (hex)	7914e27a08f6ae8eb1b96279af0db6da1da8e517
schema:url	https://doi.org/10.1007/s11596-007-0301-0
resource representing a document's title	The immunity induced by recombinant spike proteins of SARS coronavirus in Balb/c mice
has PubMed Central identifier	PMC7088728
has PubMed identifier	17641827
schema:publication	J Huazhong Univ Sci Technolog Med Sci
resource representing a document's body	covid:7914e27a08f6ae8eb1b96279af0db6da1da8e517#body_text
is schema:about of	named entity '2814' named entity 'mice' named entity 'IgG2b' named entity 'immunized' named entity 'interval' named entity 'IgG1' named entity 'fragments' covid:arg/7914e27a08f6ae8eb1b96279af0db6da1da8e517 named entity 'SARS coronavirus' named entity 'Summary' named entity 'high' named entity 'And' named entity 'change' named entity 'IFN-γ' named entity 'protein' named entity 'Serum' named entity 'severe acute respiratory syndrome coronavirus' named entity 'IgG2a' named entity 'spike protein' named entity 'titer' named entity 'protein' named entity 'IL-2' named entity 'immune effect' named entity 'spike protein' named entity 'expression vector' named entity 'Recombinant proteins' named entity 'cloned' named entity 'SARS coronavirus' named entity 'metal ion' named entity 'spleen' named entity 'Balb/c' named entity 'E.coli' named entity 'titer' named entity 'IL-4' named entity 'IgG3' named entity 'IgG1' named entity 'serum' named entity 'recombinant protein' named entity 'Balb/c' named entity 'Recombinant' named entity 'SARS' named entity 'purified' named entity 'spike' named entity 'CD8' named entity 'lymphocyte' named entity 'absorbance' named entity 'imidazole' named entity 'mice' named entity 'virus' named entity 'cytokines' named entity 'Protein' named entity 'Germany' named entity 'culture medium' named entity 'recombinant' named entity 'mice' named entity 'HRP' named entity 'Biotechnology' named entity 'recombinant proteins' named entity 'epitope' named entity 'virus particle' named entity 'flow cytometer' named entity 'epitopes' named entity 'IL-2' named entity 'protein' named entity 'standard laboratory conditions' named entity 'immune effect' named entity 'immunization' named entity 'SARS-CoV' named entity 'IgG2b' named entity 'tail vein' named entity 'mice' named entity 'IgG' named entity 'IPTG' named entity '1:50' named entity 'cellular membrane'

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