About: Differentiated human airway organoids to assess infectivity of emerging influenza virus

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Differentiated human airway organoids to assess infectivity of emerging influenza virus
Creator	Chan, Jasper Fuk-Woo Yuen, Kwok-Yung Wang, Dong Zhou, Jie Chu, Hin Zhao, Xiaoyu Clevers, Hans Li, Cun Wen, Lei Yuan, Shuofeng Chen, Honglin Sachs, Norman Song, Wenjun Bosco, Ho-Yin Chiu, Man To, Kai-Wang Wong, Kak, Kenneth Kwok, Vincent Poon, Man Wong, Yuen
Source	Medline; PMC
abstract	Novel reassortant avian influenza H7N9 virus and pandemic 2009 H1N1 (H1N1pdm) virus cause human infections, while avian H7N2 and swine H1N1 virus mainly infect birds and pigs, respectively. There is no robust in vitro model for assessing the infectivity of emerging viruses in humans. Based on a recently established method, we generated long-term expanding 3D human airway organoids which accommodate four types of airway epithelial cells: ciliated, goblet, club, and basal cells. We report differentiation conditions which increase ciliated cell numbers to a nearly physiological level with synchronously beating cilia readily discernible in every organoid. In addition, the differentiation conditions induce elevated levels of serine proteases, which are essential for productive infection of human influenza viruses and low-pathogenic avian influenza viruses. We also established improved 2D monolayer culture conditions for the differentiated airway organoids. To demonstrate the ability of differentiated airway organoids to identify human-infective virus, 3D and 2D differentiated airway organoids are applied to evaluate two pairs of viruses with known distinct infectivity in humans, H7N9/Ah versus H7N2 and H1N1pdm versus an H1N1 strain isolated from swine (H1N1sw). The human-infective H7N9/Ah virus replicated more robustly than the poorly human-infective H7N2 virus; the highly human-infective H1N1pdm virus replicated to a higher titer than the counterpart H1N1sw. Collectively, we developed differentiated human airway organoids which can morphologically and functionally simulate human airway epithelium. These differentiated airway organoids can be applied for rapid assessment of the infectivity of emerging respiratory viruses to human.
has issue date	2018-06-26(xsd:dateTime)
bibo:doi	10.1073/pnas.1806308115
bibo:pmid	29891677
has license	cc-by-nc-nd
sha1sum (hex)	793bb8ec176e4a5df8323e0bd2276e134b958b5f
schema:url	https://doi.org/10.1073/pnas.1806308115
resource representing a document's title	Differentiated human airway organoids to assess infectivity of emerging influenza virus
has PubMed Central identifier	PMC6042130
has PubMed identifier	29891677
schema:publication	Proc Natl Acad Sci U S A
resource representing a document's body	covid:793bb8ec176e4a5df8323e0bd2276e134b958b5f#body_text
is schema:about of	named entity 'BASED' named entity 'TO IDENTIFY' named entity 'TYPES' named entity 'SWINE' named entity 'INFLUENZA VIRUS' named entity 'REPORT' named entity 'AIRWAY EPITHELIUM' named entity 'ORGANOIDS' named entity 'RESPIRATORY VIRUSES' named entity 'PROXIMAL' named entity 'INFECTIONS' named entity 'INCREASE' named entity 'MONOLAYER CULTURE' named entity 'VIRUS' named entity 'METHOD' named entity 'CILIATED' named entity 'LONG-TERM' named entity 'THESE' named entity 'birds' named entity 'infectivity' named entity 'club' named entity 'differentiated' named entity 'human' named entity 'organoid' named entity 'H7N9' named entity 'infectivity' named entity 'proteases' named entity 'Novel' named entity 'HIGHER' named entity 'INFECTION' named entity 'NUMBERS' named entity 'HUMANS' named entity 'INFECTIVITY' named entity 'ASSESSING' named entity 'ASSESS' named entity 'SIMULATE' named entity 'CAUSE' named entity 'REPLICATED' named entity 'TITER' named entity 'CONDITIONS' named entity 'IMPROVED' named entity 'PATHOGENIC' named entity 'ORGANOIDS' named entity 'INFECTIVITY' named entity 'INFLUENZA VIRUS' named entity 'AIRWAY' named entity 'HUMAN' named entity 'EVALUATE' named entity 'EPITHELIAL CELLS' named entity 'CILIA' named entity 'ABILITY' named entity 'APPLIED' named entity 'PIGS' named entity 'SERINE PROTEASES' named entity 'AVIAN INFLUENZA' named entity 'INFECT' named entity 'VIRUSES' named entity 'DIFFERENTIATED' named entity 'ORGANOID' named entity 'H7N9' named entity 'INFLUENZA VIRUSES'

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