About: Antibody germline characterization of cross-neutralizing human IgGs against 4 serotypes of dengue virus

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About: Antibody germline characterization of cross-neutralizing human IgGs against 4 serotypes of dengue virus Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Antibody germline characterization of cross-neutralizing human IgGs against 4 serotypes of dengue virus
Creator	Okabayashi, Tamaki Kuhara, Motoki Benjathummarak, Surachet Fujiyama, Kazuhito Misaki, Ryo Pipattanaboon, Chonlatip Pitaksajjakul, Pannamthip Ramasoota, Pongrama Wongwit, Waranya
Source	Elsevier; Medline; PMC
abstract	Abstract Dengue virus (DENV), a re-emerging virus, constitutes the largest vector-borne disease virus, with 50–100 million cases reported every year. Although DENV infection induces lifelong immunity against viruses of the same serotypes, the subsequent infection with the heterologous serotypes can cause more severe form of the disease, such as Dengue Haemorrhagic Fever (DHF) or Dengue Shock Syndrome (DSS). However, there is neither approved vaccine nor specific drugs available to treat this disease. In this study, previously developed 19 human monoclonal antibodies (HuMAbs) showing strong to moderate cross neutralizing activity were selected. Most of them (13/19) were targeted to domain II of envelop glycoprotein. To understand and clarify the recognition properties, the maturation mechanisms comprising Variable/Diversity/Joining (VDJ) recombination, Variable Heavy (VH)/Variable Light (VL) chain pairing, variability at junctional site, and somatic hypermutation (SHM) of those antibodies were studied and compared with their predecessor germline sequences. IMGT/V-QUEST database was applied to analyze the isolated VH and VL sequences. To confirm the correction of isolated VH/VL, 3 HuMAbs (1A10H7, 1B3B9, 1G7C2) was transiently expressed in HEK293T cell. All three clones of the expressed recombinant IgG (rIgG) showed the same binding and neutralizing activity as same as those from hybridomas. The data obtained in this study will elucidate the properties of those HuMAbs for further genetic modification, and its binding epitopes.
has issue date	2014-04-04(xsd:dateTime)
bibo:doi	10.1016/j.bbrc.2014.02.131
bibo:pmid	24637211
has license	els-covid
sha1sum (hex)	79fc574b3e87b137a0ba5a55852796e257609742
schema:url	https://doi.org/10.1016/j.bbrc.2014.02.131
resource representing a document's title	Antibody germline characterization of cross-neutralizing human IgGs against 4 serotypes of dengue virus
has PubMed Central identifier	PMC7092929
has PubMed identifier	24637211
schema:publication	Biochemical and Biophysical Research Communications
resource representing a document's body	covid:79fc574b3e87b137a0ba5a55852796e257609742#body_text
is schema:about of	named entity 'infection' named entity 'SHM' named entity 'mechanisms' named entity 'recombinant' named entity 'immunity' named entity 'specific' named entity 'sequences' named entity 'dengue virus' named entity 'human' named entity 'Antibody' covid:arg/79fc574b3e87b137a0ba5a55852796e257609742 named entity 'clones' named entity 'drugs' named entity 'viruses' named entity 'genetic modification' named entity 'neutralizing' named entity 'confirm' named entity 'infection' named entity 'Dengue virus' named entity 'isolated' named entity 'Syndrome' named entity 'serotypes' named entity 'human monoclonal antibodies' named entity 'somatic hypermutation' named entity 'recombinant' named entity '100 million' named entity 'glycoprotein' named entity 'infection' named entity 'VDJ' named entity 'germline' named entity 'serotypes' named entity 'genetic modification' named entity 'dengue virus' named entity 'Antibody' named entity 'recombinant DNA' named entity 'DENV' named entity 'flu-like illness' named entity 'secondary infection' named entity '96 well plate' named entity 'SARS CoV' named entity 'anti-viral drug' named entity 'IgG' named entity 'germline' named entity 'sodium pyruvate' named entity 'serotypes' named entity 'antibodies' named entity 'heavy chain' named entity 'Promega' named entity 'IgM' named entity 'cytomegalovirus' named entity 'culture medium' named entity 'dengue' named entity 'subcloning' named entity 'Influenza virus' named entity 'germline' named entity 'constant region' named entity 'germline' named entity 'HIV' named entity 'antibody' named entity 'WNV' named entity 'MAbs' named entity '293T' named entity 'DNA Data Bank of Japan' named entity 'viral load' named entity 'germline' named entity 'non-structural proteins' named entity 'Primary infection' named entity 'DENV' named entity 'cDNA' named entity 'antibody constant region' named entity 'RSV' named entity 'virus' named entity 'virus'

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