About: Detection of 12 respiratory viruses by duplex real time PCR assays in respiratory samples

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Detection of 12 respiratory viruses by duplex real time PCR assays in respiratory samples
Creator	Arvia, Rosaria Azzi, Alberta Ciccone, Nunziata Corcioli, Fabiana Della Malva, Nunzia
source	Elsevier; Medline; PMC
abstract	Abstract Different viruses can be responsible for similar clinical manifestations of respiratory infections. Thus, the etiological diagnosis of respiratory viral diseases requires the detection of a large number of viruses. In this study, 6 duplex real-time PCR assays, using EvaGreen intercalating dye, were developed to detect 12 major viruses responsible for respiratory diseases: influenza A and B viruses, enteroviruses (including enterovirus spp, and rhinovirus spp), respiratory syncytial virus, human metapneumovirus, coronaviruses group I (of which CoV 229E and CoV NL63 are part) and II (including CoV OC43 and CoV HKU1), parainfluenza viruses type 1, 2, 3 and 4, human adenoviruses and human bocaviruses. The 2 target viruses of each duplex reaction were distinguishable by the melting temperatures of their amplicons. The 6 duplex real time PCR assays were applied for diagnostic purpose on 202 respiratory samples from 157 patients. One hundred fifty-seven samples were throat swabs and 45 were bronchoalveolar lavages. The results of the duplex PCR assays were confirmed by comparison with a commercial, validated, assay; in addition, the positive results were confirmed by sequencing. The analytical sensitivity of the duplex PCR assays varied from 103 copies/ml to 104 copies/ml. For parainfluenza virus 2 only it was 105 copies/ml. Seventy clinical samples (35%) from 55 patients (30 children and 25 adults) were positive for 1 or more viruses. In adult patients, influenza A virus was the most frequently detected respiratory virus followed by rhinoviruses. In contrast, respiratory syncytial virus was the most common virus in children, followed by enteroviruses, influenza A virus and coronavirus NL63. The small number of samples/patients does not allow us to draw any epidemiological conclusion. Altogether, the results of this study indicate that the 6 duplex PCR assays described in this study are sensitive, specific and cost-effective. Thus, this assay could be particularly useful to identify the main respiratory viruses directly from clinical samples, after nucleic acid extraction, and, also, to screen a large number of patients for epidemiological studies.
has issue date	2015-12-31(xsd:dateTime)
bibo:doi	10.1016/j.mcp.2015.08.006
bibo:pmid	26334289
has license	els-covid
sha1sum (hex)	7a487e5cda0d3abdadee013502604c795cb0f736
schema:url	https://doi.org/10.1016/j.mcp.2015.08.006
resource representing a document's title	Detection of 12 respiratory viruses by duplex real time PCR assays in respiratory samples
has PubMed Central identifier	PMC7127684
has PubMed identifier	26334289
schema:publication	Molecular and Cellular Probes
resource representing a document's body	covid:7a487e5cda0d3abdadee013502604c795cb0f736#body_text
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