About: Construction of enhanced transcriptional activators for improving cellulase production in Trichoderma reesei RUT C30

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About: Construction of enhanced transcriptional activators for improving cellulase production in Trichoderma reesei RUT C30 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Construction of enhanced transcriptional activators for improving cellulase production in Trichoderma reesei RUT C30
Creator	Wang, Wei Wei, Dongzhi Wu, Chuan Zhang, Jiajia
Source	PMC
abstract	Enhancing cellulase production in Trichoderma reesei is of great interest for an economical biorefinery. Artificial transcription factors are a potentially powerful molecular strategy for improving cellulase production in T. reesei. In this study, enhanced transcriptional activators XYR1VP, ACE2VP, and ACE1VP were constructed by linking the C terminus of XYR1, ACE2, or ACE1 with an activation domain of herpes simplex virus protein VP16. T. reesei transformants T(XYR1VP), T(ACE2VP), and T(ACE1VP) showed improved cellulase and/or xylanase production. T(XYR1VP) has a cellulase-free phenotype but with significantly elevated xylanase production. Xylanase I and xylanase II activities [U/(mg biomass)] increased by 51% and 80%, respectively, in T(XYR1VP) in comparison with parental strain RUT C30. The filter paper activity of T(ACE2VP) in the Avicel-based medium increased by 52% compared to that of RUT C30. In the Avicel-based medium, T(ACE1VP) manifested an 80% increase in FPase activity and a 50% increase in xylanase activity as compared to those of RUT C30. Additionally, when pretreated corn stover was hydrolyzed, crude enzymes produced from T(ACE1VP) yielded a greater glucose release than did the enzymes produced by parental strain RUT C30. [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s40643-018-0226-4) contains supplementary material, which is available to authorized users.
has issue date	2018-08-18(xsd:dateTime)
bibo:doi	10.1186/s40643-018-0226-4
bibo:pmid	32288986
has license	cc-by
sha1sum (hex)	7a4e535f5a06c7f61a0d01cec2ea3d3d5b853888
schema:url	https://doi.org/10.1186/s40643-018-0226-4
resource representing a document's title	Construction of enhanced transcriptional activators for improving cellulase production in Trichoderma reesei RUT C30
has PubMed Central identifier	PMC7101855
has PubMed identifier	32288986
schema:publication	Bioresour Bioprocess
resource representing a document's body	covid:7a4e535f5a06c7f61a0d01cec2ea3d3d5b853888#body_text
is schema:about of	named entity 'phenotype' named entity 'ACE2' named entity 'medium' named entity 'cellulase' named entity 'Trichoderma reesei' named entity 'ORIGINAL' named entity 'BASED' named entity 'INCREASE' named entity 'CELLULASE' named entity 'RELEASE' named entity 'GREAT' named entity 'ACTIVATION DOMAIN' named entity 'CREDIT' named entity 'REPRODUCTION' named entity 'VIRUS PROTEIN' named entity 'PRODUCTION' named entity 'Xylanase' named entity 'economical' named entity 'provide' named entity 'elevated' named entity 'medium' named entity 'C terminus' named entity 'C30' named entity 'Artificial' named entity 'C30' named entity 'improved' named entity 'RUT' named entity 'production' named entity 'improving' named entity 'Artificial transcription factors' named entity 'xylanase' named entity 'ACE2' named entity 'cellulase' named entity 'transcriptional activators' named entity 'T. reesei' named entity 'biomass' named entity 'cellulase' named entity 'Xylanase' named entity 'protein' named entity 'cellulase' named entity 'glucose' named entity 'VP16' named entity 'cellulase' named entity 'cellulase' named entity 'Cre recombinase' named entity 'promoter regions' named entity 'mycelia' named entity 'cellulase' named entity 'xylanase' named entity 'cellulase' named entity 'ace2' named entity 'β-glucosidase' named entity 'cellulase' named entity 'cellulase' named entity 'homologous' named entity 'phenotype' named entity 'subculturing' named entity 'carboxymethyl cellulose' named entity 'T. reesei' named entity 'cellulase' named entity 'hydrolase' named entity 'centrifugation' named entity 'SuperMix' named entity 'TFs' named entity 'Beijing' named entity 'cellulase' named entity 'gene expression' named entity 'TFs' named entity 'ACE2' named entity 'gene expression' named entity 'binding domain' named entity 'lactose' named entity 'cellulase' named entity 'gene' named entity 'enzyme' named entity 'hydrolase' named entity 'Novozymes' named entity 'technical replicates'

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