About: A sensitive and affordable multiplex RT-qPCR assay for SARS-CoV-2 detection

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About: A sensitive and affordable multiplex RT-qPCR assay for SARS-CoV-2 detection Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A sensitive and affordable multiplex RT-qPCR assay for SARS-CoV-2 detection
Creator	Haas, Jürgen Tait-Burkard, Christine Moore, David Templeton, Kate Jackson, Andrew Sanchez, Maria Tomlinson, Ian Johannessen, Ingolfur Acosta, Juan Adamowicz, Martyna Adams, Ian Ansari, Morad Black, Holly Chee, Mow Daniels, Alison Diamond, Austin Drake, Camilla Friman, Elias Gilbert, Nick Hurd, Toby Jarman, Edward Li, Frederic Longman, Dasa Mckay, Stewart Mcnab, Michelle Mordstein, Christine O'shea, Marie Rainger, Jacqueline Reijns, Martin Sanchez-Luque, Francisco Shepherd, Jill Slater, Louise Thompson, Louise Uggenti, Carolina Walker, Marion Warlow, Sophie Wilkinson, Nadine
source	MedRxiv
abstract	With the ongoing COVID-19 pandemic, caused by the novel coronavirus SARS-CoV-2, there is need for sensitive, specific and affordable diagnostic tests to identify infected individuals, not all of whom are symptomatic. The most sensitive test involves the detection of viral RNA using RT-qPCR, with many commercial kits now available for this purpose. However, these are expensive and supply of such kits in sufficient numbers cannot always be guaranteed. We therefore developed a multiplex assay using well-established SARS-CoV-2 targets alongside internal controls that monitor sample quality and nucleic acid extraction efficiency. Here, we establish that this test performs as well as widely used commercial assays, but at substantially reduced cost. Furthermore, we demonstrate >1,000-fold variability in material routinely collected by nose-and-throat swabbing. The inclusion of a human control probe in our assay provides additional information that could help reduce false negative rates.
has issue date	2020-07-16(xsd:dateTime)
bibo:doi	10.1101/2020.07.14.20154005
has license	medrxiv
sha1sum (hex)	7b20ab95e844130487bf1d2bff207ccac7ad81b2
schema:url	https://doi.org/10.1101/2020.07.14.20154005
resource representing a document's title	A sensitive and affordable multiplex RT-qPCR assay for SARS-CoV-2 detection
resource representing a document's body	covid:7b20ab95e844130487bf1d2bff207ccac7ad81b2#body_text
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