About: Enumeration of isotype-specific antibody-secreting cells derived from gnotobiotic piglets inoculated with porcine rotaviruses

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About: Enumeration of isotype-specific antibody-secreting cells derived from gnotobiotic piglets inoculated with porcine rotaviruses Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Enumeration of isotype-specific antibody-secreting cells derived from gnotobiotic piglets inoculated with porcine rotaviruses
Creator	Campbel12, Thomas Chen', Wei-Kang Saip, Linda Vancott3, John
Source	Elsevier; Medline; PMC
abstract	Abstract In order to evaluate mucosal antibody responses to rotavirus, an enzyme-linked immunospot (ELISPOT) assay was adapted to enumerate antibody-secreting cells (ASC) in the mesenteric lymph nodes (MLN), lamina propria (LP) of the small intestine and spleens of gnotobiotic pigs orally inoculated with porcine rotaviruses (SB1A and Gottfried). Rotavirus-specific IgM ASC occurred by post-inoculation day (PID) 3, and numbers peaked in spleen and MLN tissues by PID 7 and in intestinal LP by PID 7–14. Numbers of rotavirus specific IgA and IgG ASC in these tissues peaked at PID 14–21. Rotavirus specific IgA ASC were predominant in the gut and IgA to IgG rotavirus specific ASC ratios were highest for all rotavirus antigen coatings in the gut LP. However, the relative ratios of specific IgA to IgG ASC were lower (ratios of 5 to 7) against combined structural and nonstructural viral antigens (rotavirus-infected fixed cell ELISPOT plates) than ratios (13 to 46) against only viral structural antigens (rotavirus-coated ELISPOT plates), indicating that there were proportionately more specific IgG ASC to the nonstructural viral antigens in the LP, the tissue adjacent to the site of rotavirus replication in the intestine. In the node cells (spleen and MLN) rotavirus-specific IgA to IgG ASC ratios were lowest and against the various ELISPOT rotavirus coatings ranged from 0.7 to 4. Gnotobiotic piglets inoculated at different ages with porcine rotaviruses generally showed similar specific immunoglobulin (Ig) ASC responses to rotavirus infection, along with similar diarrhea and virus shedding patterns in the different age groups. However, the numbers of specific IgA ASC in the MLN of 3–4 week old pigs were higher than those of 3–5 day old pigs. Although challenge of SB1A or Gottfried rotavirus-inoculated pigs with SB1A (G4P7) or Gottfried (G4P6) rotavirus revealed a high degree of protection from diarrhea and virus shedding, greater numbers of specific IgM ASC were observed in spleen after challenge of SB1A-inoculated pigs with Gottfried rotavirus (same G type, distinct P type). Thus, by using the ELISPOT technique, we successfully measured intestinal mucosal antibody-related responses to rotavirus in gnotobiotic pigs. Moreover, our results support the use of gnotobiotic piglets as an animal model to evaluate active antibody responses and protection against rotavirus infection and disease.
has issue date	1995-04-30(xsd:dateTime)
bibo:doi	10.1016/0165-2427(94)05343-q
bibo:pmid	7676610
has license	els-covid
sha1sum (hex)	7bbe4bc49ad4817bf80b5bde3884fc1e1c9bc548
schema:url	https://doi.org/10.1016/0165-2427%2894%2905343-q
resource representing a document's title	Enumeration of isotype-specific antibody-secreting cells derived from gnotobiotic piglets inoculated with porcine rotaviruses
has PubMed Central identifier	PMC7119826
has PubMed identifier	7676610
schema:publication	Veterinary Immunology and Immunopathology
resource representing a document's body	covid:7bbe4bc49ad4817bf80b5bde3884fc1e1c9bc548#body_text
is schema:about of	named entity 'Immunology' named entity 'FREE' named entity 'FUNDED' named entity 'MEANS' named entity 'REPOSITORIES' named entity 'DATABASE' covid:arg/7bbe4bc49ad4817bf80b5bde3884fc1e1c9bc548 named entity 'SECRETING' named entity 'LONG' named entity 'USE' named entity 'PERMISSIONS' named entity 'PORCINE' named entity 'SPECIFIC ANTIBODY' named entity 'DERIVED' named entity 'RESOURCE' named entity 'VETERINARY IMMUNOLOGY' named entity 'ORIGINAL SOURCE' named entity 'COVID-19' named entity 'THESE' named entity 'ACTIVE' named entity 'UNRESTRICTED' named entity 'ROTAVIRUSES' named entity 'FORM' named entity '29265' named entity 'IMMUNOPATHOLOGY' named entity 'RESEARCH' named entity 'CENTRE' named entity 'RIGHTS' named entity 'ENUMERATION' named entity 'ISOTYPE' named entity 'CELLS' named entity 'COVID' named entity 're-use' named entity 'derived' named entity 'ELSEVIER' named entity 'immunopathology' named entity 'Immunopathology' named entity 'porcine' named entity 'antibody' named entity 'immunology' named entity 'original source' named entity 'isotype' named entity 'resource centre' named entity 'COVID-19' named entity 'repositories' named entity 'database' named entity 'gnotobiotic' named entity 'Immunology' named entity 'inoculated' named entity 'Elsevier' named entity 'permissions' named entity 'Veterinary' named entity 'Elsevier' named entity 'ELSEVIER' named entity 'granted' named entity 'piglets' named entity 'cells' named entity 'long' named entity 'database' named entity 'publicly funded' named entity 'Immunopathology' named entity 'immunology' named entity 'original' named entity 'active' named entity 'immunopathology' named entity 'Veterinary' named entity 'porcine' named entity 'inoculated' named entity 'Enumeration' named entity 'REMAINS' nodeID://b56406194 nodeID://b56414026 nodeID://b56456714 nodeID://b56460074 nodeID://b56464606 nodeID://b56471306 nodeID://b56479782 nodeID://b56480850

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