About: TRPM8 Channel Activation Reduces the Spontaneous Contractions in Human Distal Colon

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About: TRPM8 Channel Activation Reduces the Spontaneous Contractions in Human Distal Colon Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	TRPM8 Channel Activation Reduces the Spontaneous Contractions in Human Distal Colon
Creator	Amato, Antonella Lentini, Laura Marchesa, Pierenrico Mulè, Flavia Terzo, Simona Benfratelli, Cristina Di, Civico Oncology Hospital, U Ospedali, A Palermo,
Source	PMC
abstract	The transient receptor potential-melastatin 8 (TRPM8) is a non-selective Ca(2+)-permeable channel, activated by cold, membrane depolarization, and different cooling compounds. TRPM8 expression has been found in gut mucosal, submucosal, and muscular nerve endings. Although TRPM8 plays a role in pathological conditions, being involved in visceral pain and inflammation, the physiological functions in the digestive system remain unclear as yet. The aims of the present study were: (i) to verify the TRPM8 expression in human distal colon; (ii) to examine the effects of TRPM8 activation on colonic contractility; (iii) to characterize the mechanism of action. Reverse transcriptase-polymerase chain reaction (RT-PCR) and western blotting were used to analyze TRPM8 expression. The responses of human colon circular strips to different TRPM8 agonists [1-[Dialkyl-phosphinoyl]-alkane (DAPA) 2–5, 1-[Diisopropyl-phosphinoyl]-alkane (DIPA) 1–7, DIPA 1–8, DIPA 1–9, DIPA 1–10, and DIPA 1–12) were recorded using a vertical organ bath. The biomolecular analysis revealed gene and protein expression of TRPM8 in both mucosal and smooth muscle layers. All the agonists tested, except-DIPA 1–12, produced a concentration-dependent decrease in spontaneous contraction amplitude. The effect was significantly antagonized by 5-benzyloxytryptamine, a TRPM8 antagonist. The DIPA 1–8 agonist resulted in the most efficacious and potent activation among the tested molecules. The DIPA 1–8 effects were not affected by tetrodotoxin, a neural blocker, but they were significantly reduced by tetraethylammonium chloride, a non-selective blocker of K(+) channels. Moreover, iberiotoxin, a blocker of the large-conductance Ca(2+)-dependent K(+)-channels, but not apamin, a blocker of small-conductance Ca(2+)-dependent K(+) channels, significantly reduced the inhibitory DIPA 1–8 actions. The results of the present study demonstrated that TRPM8 receptors are also expressed in human distal colon in healthy conditions and that ligand-dependent TRPM8 activation is able to reduce the colonic spontaneous motility, probably by the opening of the large-conductance Ca(2+)-dependent K(+)-channels.
has issue date	2020-07-29(xsd:dateTime)
bibo:doi	10.3390/ijms21155403
bibo:pmid	32751347
has license	cc-by
sha1sum (hex)	7bffbbdb46519bdc831956f7a5b224b0e638d893
schema:url	https://doi.org/10.3390/ijms21155403
resource representing a document's title	TRPM8 Channel Activation Reduces the Spontaneous Contractions in Human Distal Colon
has PubMed Central identifier	PMC7432081
has PubMed identifier	32751347
schema:publication	Int J Mol Sci
resource representing a document's body	covid:7bffbbdb46519bdc831956f7a5b224b0e638d893#body_text
is schema:about of	named entity 'gene' named entity 'colon' named entity 'demonstrated' named entity 'colonic' named entity 'reduced' named entity 'spontaneous' named entity 'tested' named entity 'TRPM8' named entity 'Distal' named entity 'SMALL' named entity 'COOLING' named entity '1-10' named entity 'CONCENTRATION' named entity 'RECEPTOR' named entity 'SUBMUCOSAL' named entity 'TRANSIENT' named entity 'PCR' named entity 'BEING' named entity 'nerve endings' named entity 'permeable' named entity 'channels' named entity 'efficacious' named entity 'receptor' named entity 'plays' named entity 'functions' named entity 'TRPM8' named entity 'expression' named entity 'opening' named entity 'TRPM8' named entity 'study' named entity 'compounds' named entity 'channels' named entity 'RT-PCR' named entity 'effects' named entity 'dependent' named entity 'mucosal' named entity 'TRPM8' named entity 'tetraethylammonium chloride' named entity 'TRPM8' named entity 'TRPM8' named entity 'distal colon' named entity 'mechanism of action' named entity 'agonists' named entity 'animal models' named entity 'DED' named entity 'TRPM8' named entity 'alkane' named entity 'depolarization' named entity 'extracellular' named entity 'KCl' named entity 'academic research' named entity 'RNA' named entity '5-benzyloxytryptamine' named entity 'smooth muscle' named entity 'smooth muscle' named entity 'colitis' named entity 'Student's t-test' named entity 'permeation' named entity 'motility' named entity 'steric hindrance' named entity 'TRPM8' named entity 'IBD' named entity 'muscle' named entity 'heptyl' named entity 'TRPM8' named entity 'TRPM8' named entity 'TRPM8' named entity 'pre-stained' named entity 'TRPM8' named entity 'Bio-Rad' named entity 'mucosa' named entity 'TRPM8'

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