About: Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors

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About: Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors
Creator	Cerovska, Noemi Hitzeroth, Inga Moravec, Tomas Plchova, Helena Poborilova, Zuzana »more»
source	PMC
abstract	KEY MESSAGE: This is the first evidence that replicating vectors can be successfully used for transient protein expression in BY-2 plant cell packs. ABSTRACT: Transient recombinant protein expression in plants and recently also plant cell cultures are of increasing interest due to the speed, safety and scalability of the process. Currently, studies are focussing on the design of plant virus-derived vectors to achieve higher amounts of transiently expressed proteins in these systems. Here we designed and tested replicating single and multi-cassette vectors that combine elements for enhanced replication and hypertranslation, and assessed their ability to express and particularly co-express proteins by Agrobacterium-mediated transient expression in tobacco BY-2 plant cell packs. Substantial yields of green and red fluorescent proteins of up to ~ 700 ng/g fresh mass were detected in the plant cells along with position-dependent expression. This is the first evidence of the ability of replicating vectors to transiently express proteins in BY-2 plant cell packs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00299-020-02544-w) contains supplementary material, which is available to authorized users.
has issue date	2020-04-24(xsd:dateTime)
bibo:doi	10.1007/s00299-020-02544-w
bibo:pmid	32333151
has license	cc-by
sha1sum (hex)	7ccb598e9de4535d5f8eb906b898db4d73edb921
schema:url	https://doi.org/10.1007/s00299-020-02544-w
resource representing a document's title	Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors
has PubMed Central identifier	PMC7223956
has PubMed identifier	32333151
schema:publication	Plant Cell Rep
resource representing a document's body	covid:7ccb598e9de4535d5f8eb906b898db4d73edb921#body_text
is schema:about of	named entity 'replicating' named entity 'Key' named entity 'speed' named entity 'replicating' named entity 'EXPRESS' »more»

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