About: Molecular Diagnosis of Pneumonia Using Multiplex Real-Time PCR Assay RespiFinder® SMART 22 FAST in a Group of Moroccan Infants

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About: Molecular Diagnosis of Pneumonia Using Multiplex Real-Time PCR Assay RespiFinder® SMART 22 FAST in a Group of Moroccan Infants Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Molecular Diagnosis of Pneumonia Using Multiplex Real-Time PCR Assay RespiFinder® SMART 22 FAST in a Group of Moroccan Infants
Creator	Barkat, Amina Hattoufi, Kenza Kharbach, Aicha Obtel, Majdouline Tligui, Houssain Ftouh, Sobha
Source	Medline; PMC
abstract	BACKGROUND: In Morocco, pediatric pneumonia remains a serious public health problem, as it constitutes the first cause of mortality due to infectious diseases. The etiological diagnosis of acute respiratory tract infections is difficult. Therefore, it is necessary to use Multiplex real-time polymerase chain reaction assay tests in a routine setting for exact and fast identification. OBJECTIVES: In this paper, we present the clinical results of pediatric pneumonia and describe their etiology by using molecular diagnosis. Study design: Tracheal secretion was collected from infants presenting respiratory distress isolated or associated with systemic signs, attending the unit of Neonatology between December 1, 2016, and Mai 31, 2018. Samples were tested with the multiplex RespiFinder® SMART 22 FAST which potentially detects 18 viruses and 4 bacteria. RESULTS: Of the 86 infants considered in this study (mean age 31 ± 19 days) suspected of acute respiratory tract infections, 71 (83%) were positive for one or multiple viruses or/and bacteria. The majority of acute respiratory tract infections had a viral origin (95%): respiratory syncytial viruses (A and B) (49%), rhinovirus (21%), coronaviruses 229E (11%), humain metapneumovirus (5%), influenza A (3%), influenza H1N1 (1%), adenovirus (2%), and parainfluenza virus type 4 (2%). Among our patients, 6% had Mycoplasma pneumoniae. Coinfections were not associated with severe respiratory symptoms. CONCLUSION: The clinical spectrum of respiratory infections is complex and often nonspecific. Thus, the early and fast detection of related causative agents is crucial. The use of multiplex real time polymerase chain reaction may help choose an accurate treatment, reduce the overall use of unnecessary antibiotics, preserve intestinal flora, and decrease nosocomial infection by reducing the length of hospitalization.
has issue date	2020-02-18(xsd:dateTime)
bibo:doi	10.1155/2020/6212643
bibo:pmid	32148499
has license	cc-by
sha1sum (hex)	7e0925a800d4d09380ac44584b5be257d83531d3
schema:url	https://doi.org/10.1155/2020/6212643
resource representing a document's title	Molecular Diagnosis of Pneumonia Using Multiplex Real-Time PCR Assay RespiFinder® SMART 22 FAST in a Group of Moroccan Infants
has PubMed Central identifier	PMC7049438
has PubMed identifier	32148499
schema:publication	Adv Virol
resource representing a document's body	covid:7e0925a800d4d09380ac44584b5be257d83531d3#body_text
is schema:about of	named entity 'ADENOVIRUS' named entity 'COMPLEX' named entity 'ORIGIN' named entity 'RHINOVIRUS' named entity 'MULTIPLEX REAL-TIME POLYMERASE CHAIN REACTION ASSAY' named entity 'REAL TIME POLYMERASE CHAIN REACTION' named entity 'DUE TO' named entity '283' named entity 'MORTALITY' named entity 'CONSIDERED' named entity 'MULTIPLE' named entity 'SYSTEMIC' named entity 'TRACHEAL' named entity '282' named entity 'MULTIPLEX' named entity 'RESPIRATORY INFECTIONS' named entity 'SEVERE' named entity 'MAJORITY' named entity 'BACTERIA.' named entity 'PRESENT' named entity 'AGE' named entity 'NOSOCOMIAL INFECTION' named entity 'MULTIPLEX REAL-TIME PCR' named entity 'Mycoplasma pneumoniae' named entity 'polymerase' named entity 'multiplex' named entity 'describe' named entity 'H1N1' named entity 'chain reaction' named entity 'secretion' named entity 'detects' named entity '2018' named entity 'FAST' named entity 'HELP' named entity 'PEDIATRIC' named entity 'DIAGNOSIS' named entity 'SPECTRUM' named entity 'POSITIVE FOR' named entity 'USING' named entity 'SIGNS' named entity 'NEONATOLOGY' named entity 'RESPIRATORY TRACT INFECTIONS' named entity 'MYCOPLASMA PNEUMONIAE' named entity 'RESPIRATORY SYMPTOMS' named entity 'INTESTINAL FLORA' named entity 'REDUCING' named entity 'PUBLIC HEALTH' named entity 'REDUCE' named entity '229E' named entity 'CLINICAL' named entity 'MOLECULAR DIAGNOSIS' named entity 'MOROCCO' named entity 'DECREASE' named entity 'RESPIRATORY DISTRESS' named entity 'SMART' named entity 'RELATED' named entity 'OUR' named entity 'OVERALL' named entity 'RESPIRATORY SYNCYTIAL VIRUSES' named entity 'CONCLUSION' named entity 'OBJECTIVES' named entity 'DIFFICULT' named entity 'SAMPLES' named entity 'VIRUSES' named entity 'MAI' named entity 'USE OF' named entity 'INFLUENZA A' named entity 'SMART' named entity 'A GROUP' named entity 'MOROCCAN' named entity 'INFANTS' named entity 'REAL-TIME PCR ' named entity 'PNEUMONIA' named entity 'PCR ASSAY' named entity '2018' named entity 'INFANTS'

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