About: Production of a monoclonal antibody against SARS-CoV spike protein with single intrasplenic immunization of plasmid DNA

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About: Production of a monoclonal antibody against SARS-CoV spike protein with single intrasplenic immunization of plasmid DNA Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Production of a monoclonal antibody against SARS-CoV spike protein with single intrasplenic immunization of plasmid DNA
Creator	Li, Yan Liang, Lin Chao, Zhong Fei, Xiao Gu, Jie Liao, Xiao She, Ming
source	Elsevier; Medline; PMC
abstract	Severe acute respiratory syndrome (SARS) is a highly infectious disease caused by a novel coronavirus (SARS-CoV). Specific monoclonal antibodies (mAbs) against the SARS-CoV are vital for early diagnosis and pathological studies of SARS. Direct intrasplenic inoculation of plasmid DNA encoding antigen is an effective and fast approach to generate specific mAb when the protein antigen is difficult to prepare or dangerous in use. In this study, we selected one fragment of SARS-CoV spike protein (S1-(3)) as antigenic determinant by immunoinformatics. Single intrasplenic immunization of plasmid DNA encoding S1-(3) induced anti-spike protein antibodies. We established one hybridoma cell line secreting specific mAb and evaluated this mAb with murine leukemia virus pseudotyped with SARS-CoV spike protein (MLV/SARS-CoV). The mAb could recognize the spike protein on the MLV/SARS-CoV-infected Vero E6 cells albeit with no neutralizing effect on the infectivity of the pseudotype virus. Our results show that a single-shot intrasplenic DNA immunization is efficient for the production of specific mAb against SARS spike protein, and a linear epitope of the spike protein is recognized in this study.
has issue date	2005-09-15(xsd:dateTime)
bibo:doi	10.1016/j.imlet.2005.03.015
bibo:pmid	15893826
has license	no-cc
sha1sum (hex)	7e0e34b3d96e75b4680ab2a463e925f44c7d015b
schema:url	https://doi.org/10.1016/j.imlet.2005.03.015
resource representing a document's title	Production of a monoclonal antibody against SARS-CoV spike protein with single intrasplenic immunization of plasmid DNA
has PubMed Central identifier	PMC7112869
has PubMed identifier	15893826
schema:publication	Immunol Lett
resource representing a document's body	covid:7e0e34b3d96e75b4680ab2a463e925f44c7d015b#body_text
is schema:about of	named entity 'spike' named entity 'mAb' named entity 'virus' named entity 'secreting' named entity 'SPIKE PROTEIN' named entity 'HYBRIDOMA CELL LINE' named entity 'PATHOLOGICAL' named entity 'SPECIFIC' named entity 'PROTEIN ' named entity 'SARS' named entity 'CAUSED BY' named entity 'GENERATE' named entity 'RESULTS' named entity 'SHOT' named entity 'EFFECTIVE' named entity 'ENCODING' named entity 'EFFICIENT' named entity 'EVALUATED' named entity 'ANTIGEN' named entity 'PLASMID DNA' named entity 'STUDY' named entity 'SELECTED' named entity 'IMMUNIZATION' named entity 'MONOCLONAL ANTIBODY' named entity 'SINGLE' named entity 'SARS-COV' named entity 'PRODUCTION' named entity 'PROTEIN ' named entity 'TO PREPARE' named entity 'LINEAR' named entity 'APPROACH' named entity 'OUR' named entity 'INOCULATION' named entity 'MURINE LEUKEMIA VIRUS' named entity 'EFFECT' named entity 'INFECTED' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'USE' named entity 'MAB' named entity 'INDUCED' named entity 'CELLS' named entity 'PSEUDOTYPE VIRUS' named entity 'SECRETING' named entity 'IMMUNIZATION' named entity 'ESTABLISHED' named entity 'SPIKE PROTEIN' named entity 'PROTEIN ANTIGEN' named entity 'SINGLE' named entity 'VERO' named entity 'EPITOPE' named entity 'FRAGMENT OF' named entity 'MONOCLONAL ANTIBODIES' named entity 'IMMUNOINFORMATICS' named entity 'PLASMID DNA' named entity 'MLV' named entity 'INFECTIVITY' named entity 'EARLY DIAGNOSIS' named entity 'DNA IMMUNIZATION' named entity 'PRODUCTION' named entity 'FAST' named entity 'ANTIGENIC DETERMINANT' named entity 'STUDIES' named entity 'VITAL' named entity 'IS A' named entity 'DIRECT' named entity 'DIFFICULT' named entity 'ANTIBODIES' named entity 'INFECTIOUS DISEASE' named entity 'NOVEL CORONAVIRUS' named entity 'HIGHLY' named entity 'SARS-COV' named entity 'RECOGNIZE' covid:arg/7e0e34b3d96e75b4680ab2a463e925f44c7d015b named entity 'studies' named entity 'selected'

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