About: Evaluation of a rapid molecular algorithm for detection of pandemic influenza A (H1N1) 2009 virus and screening for a key oseltamivir resistance (H275Y) substitution in neuraminidase

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About: Evaluation of a rapid molecular algorithm for detection of pandemic influenza A (H1N1) 2009 virus and screening for a key oseltamivir resistance (H275Y) substitution in neuraminidase Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Evaluation of a rapid molecular algorithm for detection of pandemic influenza A (H1N1) 2009 virus and screening for a key oseltamivir resistance (H275Y) substitution in neuraminidase
Creator	Meijer, A Aron, G Fouchier, R Jonges, M Koopmans, M Osterhaus, A Schutten, M Bestebroer, T Boucher, C Guldemeester, J Herfst, S Maaskant, J Van Der Linden, A Van Der Vries, E
source	Elsevier; Medline; PMC
abstract	BACKGROUND: Rapid and specific molecular tests for identification of the recently identified pandemic influenza A/H1N1 2009 virus as well as rapid molecular tests to identify antiviral resistant strains are urgently needed. OBJECTIVES: We have evaluated the performance of two novel reverse transcriptase polymerase chain reactions (RT-PCRs) targeting specifically hemagglutinin and neuraminidase of pandemic influenza A/H1N1 virus in combination with a conserved matrix PCR. In addition, we investigated the performance of a novel discrimination RT-PCR for detection of the H275Y resistance mutation in the neuraminidase gene. STUDY DESIGN: Clinical performance of both subtype specific RT-PCR assays was evaluated through analysis of 684 throat swaps collected from individuals meeting the WHO case definition for the novel pandemic influenza virus. Analytical performance was analyzed through testing of 10-fold serial dilutions of RNA derived from the first Dutch sequenced and cultured confirmed case of novel pandemic influenza infection. Specificity and discriminative capacities of the H275Y discrimination assay were performed by testing wild type and recombinant H275Y pandemic influenza. RESULTS: 121 throat swaps collected from April 2009 to July 2009 were positive by at least two out of three RT-PCRs, and negative for the seasonal H3/H1 subtype specific RT-PCR assays. 117 of these were tested positive for all three (Ct-values from 15.1 to 36.8). No oseltamivir resistance was detected. CONCLUSIONS: We present a sensitive and specific approach for detection of pandemic influenza A/H1N1 2009 and a rapid RT-PCR assay detecting a primary oseltamivir resistance mutation which can be incorporated easily into clinical virology algorithms.
has issue date	2009-10-25(xsd:dateTime)
bibo:doi	10.1016/j.jcv.2009.09.030
bibo:pmid	19857993
has license	no-cc
sha1sum (hex)	7e626687c45a46198ba79971704c9a4604c824e4
schema:url	https://doi.org/10.1016/j.jcv.2009.09.030
resource representing a document's title	Evaluation of a rapid molecular algorithm for detection of pandemic influenza A (H1N1) 2009 virus and screening for a key oseltamivir resistance (H275Y) substitution in neuraminidase
has PubMed Central identifier	PMC7185517
has PubMed identifier	19857993
schema:publication	J Clin Virol
resource representing a document's body	covid:7e626687c45a46198ba79971704c9a4604c824e4#body_text
is schema:about of	named entity 'ALGORITHM' named entity 'PANDEMIC' named entity 'confirmed' named entity 'assay' named entity 'tests' named entity 'pandemic' named entity 'virus' named entity 'wild type' named entity 'resistance' named entity 'fold' named entity 'virus' named entity 'influenza A/H1N1' named entity 'algorithm' named entity 'RESISTANCE' named entity 'BACKGROUND' named entity 'MATRIX' named entity 'INFLUENZA VIRUS' named entity 'EVALUATED' named entity 'OBJECTIVES' named entity 'IDENTIFICATION' named entity 'ANALYSIS' named entity 'KEY' named entity 'RAPID' named entity 'SPECIFICITY' named entity 'RESISTANT' named entity 'CULTURED' named entity 'RT-PCR ASSAY' named entity 'RECENTLY' named entity 'EASILY' named entity 'DUTCH' named entity 'CONFIRMED CASE' named entity 'RNA' named entity 'ASSAY' named entity 'DISCRIMINATION' named entity 'INDIVIDUALS' named entity 'SEASONAL' named entity 'VALUES' named entity 'SPECIFIC' named entity 'SCREENING' named entity 'MOLECULAR' named entity 'INFLUENZA A' named entity 'ANALYTICAL' named entity 'RESULTS' named entity 'INVESTIGATED' named entity 'MUTATION' named entity 'TESTS' named entity 'NEGATIVE' named entity 'INFLUENZA INFECTION' named entity 'DETECTION' named entity 'STRAINS' named entity 'POSITIVE' named entity 'PERFORMANCE' named entity 'NEURAMINIDASE' named entity 'RAPID' named entity 'VIRUS' named entity 'EVALUATION' named entity 'SUBSTITUTION' named entity 'THROAT' named entity 'NOVEL' named entity 'RT-PCR' named entity 'FOLD' named entity 'CASE DEFINITION' named entity 'PRESENT' named entity 'ALGORITHMS' named entity 'PRIMARY' named entity 'SERIAL' named entity 'TARGETING' named entity 'APRIL'

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