About: Determination of host proteins composing the microenvironment of coronavirus replicase complexes by proximity-labeling

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Determination of host proteins composing the microenvironment of coronavirus replicase complexes by proximity-labeling Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Determination of host proteins composing the microenvironment of coronavirus replicase complexes by proximity-labeling
Creator	Thiel, Volker Dijkman, Ronald Kirkegaard, Karla Stalder, Hanspeter Gerber, Markus Ebert, Nadine Heller, Manfred Kelly, Jenna Pfaender, Stephanie Portmann, Jasmine Simillion, Cedric Stoffel, Michael V'kovski, Philip Lagache, Sophie My Bruggmann, Ré Ronique Gaschen, Vé
Source	Medline; PMC
abstract	Positive-sense RNA viruses hijack intracellular membranes that provide niches for viral RNA synthesis and a platform for interactions with host proteins. However, little is known about host factors at the interface between replicase complexes and the host cytoplasm. We engineered a biotin ligase into a coronaviral replication/transcription complex (RTC) and identified >500 host proteins constituting the RTC microenvironment. siRNA-silencing of each RTC-proximal host factor demonstrated importance of vesicular trafficking pathways, ubiquitin-dependent and autophagy-related processes, and translation initiation factors. Notably, detection of translation initiation factors at the RTC was instrumental to visualize and demonstrate active translation proximal to replication complexes of several coronaviruses. Collectively, we establish a spatial link between viral RNA synthesis and diverse host factors of unprecedented breadth. Our data may serve as a paradigm for other positive-strand RNA viruses and provide a starting point for a comprehensive analysis of critical virus-host interactions that represent targets for therapeutic intervention.
has issue date	2019-01-11(xsd:dateTime)
bibo:doi	10.7554/elife.42037
bibo:pmid	30632963
has license	cc-by
sha1sum (hex)	7ec36d372303be7d61deb6a7ef724495a6d90614
schema:url	https://doi.org/10.7554/elife.42037
resource representing a document's title	Determination of host proteins composing the microenvironment of coronavirus replicase complexes by proximity-labeling
has PubMed Central identifier	PMC6372286
has PubMed identifier	30632963
schema:publication	eLife
resource representing a document's body	covid:7ec36d372303be7d61deb6a7ef724495a6d90614#body_text
is schema:about of	named entity 'LABELING' named entity 'active' named entity 'coronaviruses' named entity 'platform' named entity 'host' named entity 'replication' named entity 'replicase' named entity 'represent' named entity 'RNA' named entity 'interactions' named entity 'proteins' named entity 'CORONAVIRUS' named entity 'COMPLEXES' named entity 'DETERMINATION' named entity 'REPLICASE' named entity 'SILENCING' named entity 'PROCESSES' named entity 'LINK' named entity 'KNOWN' named entity 'POSITIVE-SENSE RNA VIRUSES' named entity 'CYTOPLASM' named entity 'VIRAL RNA' named entity 'ESTABLISH' named entity 'REPLICASE' named entity 'PARADIGM' named entity 'DETECTION' named entity 'TRANSLATION' named entity 'THERAPEUTIC INTERVENTION' named entity 'INTERFACE' named entity 'DIVERSE' named entity 'PROXIMAL' named entity 'TRANSLATION INITIATION' named entity 'ENGINEERED' named entity 'POSITIVE' named entity 'IDENTIFIED' named entity 'ABOUT' named entity 'SPATIAL' named entity 'REPRESENT' named entity 'CRITICAL' named entity 'COMPOSING' named entity 'PROTEINS' named entity 'PROXIMITY' named entity 'LITTLE' named entity 'REPLICATION' named entity 'UBIQUITIN' named entity 'INSTRUMENTAL' named entity 'DEPENDENT' named entity 'PATHWAYS' named entity 'INTRACELLULAR MEMBRANES' named entity 'MICROENVIRONMENT' named entity 'MICROENVIRONMENT' named entity 'OF EACH' named entity 'COMPREHENSIVE ANALYSIS' named entity 'COMPLEXES' named entity 'RNA SYNTHESIS' named entity 'OUR' named entity 'ACTIVE' named entity 'TARGETS' named entity 'VESICULAR' named entity 'PROTEINS' named entity 'PROVIDE' named entity 'INITIATION FACTORS' named entity 'SIRNA' named entity 'PLATFORM' named entity 'RNA VIRUSES' named entity 'SERVE'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software