About: Camelid VHHs Fused to Human Fc Fragments Provide Long Term Protection Against Botulinum Neurotoxin A in Mice

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About: Camelid VHHs Fused to Human Fc Fragments Provide Long Term Protection Against Botulinum Neurotoxin A in Mice Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Camelid VHHs Fused to Human Fc Fragments Provide Long Term Protection Against Botulinum Neurotoxin A in Mice
Creator	Logunov, Denis Naroditsky, Boris Shcheblyakov, Dmitry Solovyev, Andrey Tukhvatulin, Amir Esmagambetov, Ilias Gintsburg, Aleksandr Godakova, Svetlana Noskov, Anatoly Ugriumova, Galina Vinogradova, Irina
source	Medline; PMC
abstract	The bacterium Clostridium botulinum is the causative agent of botulism—a severe intoxication caused by botulinum neurotoxin (BoNT) and characterized by damage to the nervous system. In an effort to develop novel C. botulinum immunotherapeutics, camelid single-domain antibodies (sdAbs, VHHs, or nanobodies) could be used due to their unique structure and characteristics. In this study, VHHs were produced using phage display technology. A total of 15 different monoclonal VHHs were selected based on their comlementarity-determining region 3 (CDR3) sequences. Different toxin lethal dose (LD(50)) challenges with each selected phage clone were conducted in vivo to check their neutralizing potency. We demonstrated that modification of neutralizing VHHs with a human immunoglobulin G (IgG)1 Fc (fragment crystallizable) fragment (fusionbody, VHH-Fc) significantly increased the circulation time in the blood (up to 14 days). At the same time, VHH-Fc showed the protective activity 1000 times higher than monomeric form when challenged with 5 LD(50). Moreover, VHH-Fcs remained protective even 14 days after antibody administration. These results indicate that this VHH-Fc could be used as an effective long term antitoxin protection against botulinum type A.
has issue date	2019-08-07(xsd:dateTime)
bibo:doi	10.3390/toxins11080464
bibo:pmid	31394847
has license	cc-by
sha1sum (hex)	7f3637433b7c8dff7fc35f0b5e67794877bce0c1
schema:url	https://doi.org/10.3390/toxins11080464
resource representing a document's title	Camelid VHHs Fused to Human Fc Fragments Provide Long Term Protection Against Botulinum Neurotoxin A in Mice
has PubMed Central identifier	PMC6723419
has PubMed identifier	31394847
schema:publication	Toxins (Basel)
resource representing a document's body	covid:7f3637433b7c8dff7fc35f0b5e67794877bce0c1#body_text
is schema:about of	named entity 'times' named entity 'sequences' named entity 'produced' named entity 'selected' covid:arg/7f3637433b7c8dff7fc35f0b5e67794877bce0c1 named entity 'BOTULINUM NEUROTOXIN' named entity 'DEMONSTRATED' named entity 'CHALLENGES' named entity 'TYPE A' named entity 'MODIFICATION' named entity 'CLONE' named entity 'CLOSTRIDIUM BOTULINUM' named entity 'NOVEL' named entity 'INTOXICATION' named entity 'TIME' named entity 'FCS' named entity 'SEVERE' named entity 'HIGHER' named entity 'LONG TERM' named entity 'CAUSATIVE AGENT' named entity 'RESULTS' named entity 'PHAGE DISPLAY' named entity 'DIFFERENT' named entity 'SINGLE-DOMAIN ANTIBODIES' named entity 'NERVOUS SYSTEM' named entity 'TIMES' named entity 'BASED' named entity 'BLOOD' named entity 'DISPLAY TECHNOLOGY' named entity 'USED' named entity 'SEQUENCES' named entity 'HUMAN IMMUNOGLOBULIN G' named entity 'ACTIVITY' named entity 'DAMAGE' named entity '1000' named entity 'POTENCY' named entity 'THEIR' named entity 'CIRCULATION' named entity 'IN VIVO' named entity 'CAUSED BY' named entity 'HUMAN' named entity 'FUSED TO' named entity 'PROTECTION' named entity 'CHARACTERISTICS' named entity 'STUDY' named entity 'IMMUNOTHERAPEUTICS' named entity 'BACTERIUM' named entity 'FRAGMENTS' named entity 'REGION 3' named entity 'LONG TERM' named entity 'CAMELID' named entity 'UNIQUE' named entity 'CAMELID' named entity 'EFFORT' named entity 'CHECK' named entity 'CHARACTERIZED' named entity 'DAYS' named entity 'ANTITOXIN' named entity 'ADMINISTRATION' named entity 'BOTULISM' named entity 'THESE' named entity 'COULD BE' named entity 'PHAGE' named entity 'FRAGMENT' named entity 'BOTULINUM NEUROTOXIN' named entity 'SELECTED' named entity 'ANTIBODY' named entity 'PROTECTION' named entity 'MONOCLONAL' named entity 'EFFECTIVE' named entity 'LETHAL DOSE'

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