About: Novel Microneutralization Assay for HCMV Using Automated Data Collection and Analysis

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Novel Microneutralization Assay for HCMV Using Automated Data Collection and Analysis
Creator	Smith, Larry Wloch, Mary Abai, Anna
topic	covid:7fa1b6e588709fb66cef61b3636e5e93db07f9b0#this
Source	Elsevier; Medline; PMC
abstract	In addition to being sensitive and specific, an assay for the assessment of neutralizing antibody activity from clinical trial samples must be amenable to automation for use in high-volume screening. To that effect, we developed a 96-well microplate assay for the measurement of HCMV-neutralizing activity in human sera using the HCMV-permissive human cell line HEL-299 and the laboratory strain of HCMV AD169. The degree to which neutralizing antibodies diminish HCMV infection of cells in the assay is determined by quantifying the nuclei of infected cells based on expression of the 72 kDa IE1 viral protein. Nuclear IE1 is visualized using a highly sensitive immunoperoxidase staining and the stained nuclei are counted using an automated ELISPOT analyzer. The use of Half Area 96-well microplates, with wells in which the surface area of the well bottom is half the area of a standard 96-well microplate plate, improves signal detection compared with standard microplates and economizes on the usage of indicator cells, virus, and reagents. The staining process was also streamlined by using a microplate washer and data analysis was simplified and accelerated by employing a software program that automatically plots neutralization curves and determines NT(50) values using 4-PL curve fitting. The optimized assay is not only fast and convenient, but also specific, sensitive, precise and reproducible and thus has the characteristics necessary for use in measuring HCMV neutralizing activity in the sera of vaccine trial subjects such as the recipients of Vical’s HCMV pDNA vaccine candidates.
has issue date	2007-04-01(xsd:dateTime)
bibo:doi	10.1016/j.jim.2007.02.001
bibo:pmid	17343873
has license	green-oa
sha1sum (hex)	7fa1b6e588709fb66cef61b3636e5e93db07f9b0
schema:url	https://doi.org/10.1016/j.jim.2007.02.001
resource representing a document's title	Novel Microneutralization Assay for HCMV Using Automated Data Collection and Analysis
has PubMed Central identifier	PMC1933494
has PubMed identifier	17343873
schema:publication	Journal of Immunological Methods
resource representing a document's body	covid:7fa1b6e588709fb66cef61b3636e5e93db07f9b0#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/7fa1b6e588709fb66cef61b3636e5e93db07f9b0
is schema:about of	named entity 'MICRONEUTRALIZATION ASSAY' named entity 'values' named entity 'counted' named entity 'microplates' named entity 'Novel' named entity 'data collection' named entity 'ANALYSIS' named entity 'DATA ANALYSIS' named entity 'SURFACE AREA' named entity 'INDICATOR' named entity 'USE OF' named entity 'HIGHLY SENSITIVE' named entity 'ACTIVITY' named entity 'STRAIN' named entity 'AUTOMATED' named entity 'AUTOMATION' named entity 'PROCESS' named entity 'VIRAL PROTEIN' named entity 'STAINED' named entity 'MICROPLATE WASHER' named entity 'HCMV' named entity 'NUCLEAR' named entity 'BEING' named entity 'ELISPOT' named entity 'EMPLOYING' named entity 'HCMV' covid:arg/7fa1b6e588709fb66cef61b3636e5e93db07f9b0 named entity 'AUTOMATED DATA COLLECTION' named entity 'BASED' named entity 'HCMV INFECTION' named entity 'ACCELERATED' named entity 'USING' named entity 'HEL' named entity 'REAGENTS' named entity 'MEASUREMENT' named entity 'STAINING' named entity 'HIGH' named entity 'STANDARD' named entity 'PLATE' named entity 'ASSAY' named entity 'KDA' named entity 'AUTOMATICALLY' named entity 'DIMINISH' named entity 'DETERMINED BY' named entity 'IMPROVES' named entity 'EFFECT' named entity 'SERA' named entity 'DETECTION' named entity 'DEGREE' named entity 'VIRUS' named entity 'SPECIFIC' named entity 'SIGNAL' named entity 'EXPRESSION' named entity 'NOVEL' named entity 'IMMUNOPEROXIDASE STAINING' named entity 'IE1' named entity 'SOFTWARE PROGRAM' named entity 'MICROPLATE' named entity 'ADDITION' named entity 'HUMAN' named entity 'VALUES' named entity 'HUMAN CELL LINE' named entity 'LABORATORY' named entity 'NEUTRALIZING ANTIBODIES' named entity 'CELLS' named entity 'NEUTRALIZING ANTIBODY' named entity 'USING' named entity 'SAMPLES' named entity 'NUCLEI' named entity 'WELLS' named entity 'AMENABLE' named entity 'ANALYZER' named entity 'VISUALIZED' named entity 'SENSITIVE' named entity 'ASSESSMENT' named entity 'CURVE FITTING' named entity 'CLINICAL TRIAL'

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