About: Linear B-cell epitopes in the spike and nucleocapsid proteins as markers of SARS-CoV-2 exposure and disease severity

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About: Linear B-cell epitopes in the spike and nucleocapsid proteins as markers of SARS-CoV-2 exposure and disease severity Goto Sponge NotDistinct Permalink

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Linear B-cell epitopes in the spike and nucleocapsid proteins as markers of SARS-CoV-2 exposure and disease severity
Creator	Kalimuddin, Shirin Leo, Yee-Sin Chen, Mark I-Cheng Lee, Bernett Pada, Surinder Carissimo, Guillaume Chan, Yi-Hao Tambyah, Paul Lye, David Ng, Lisa Tay, Matthew Young, Barnaby Guek, Jenny Lee, Yi-Pin Low, Hong Naqiah Amrun, Siti Poh, Chek Renia, Laurent Tan, Seow-Yen Chang, Wei Chee, Ling Fong, Siew-Wai Sin, Rhonda Sun, Louisa Torres-Ruesta, Anthony Yeo, Kim-Wah
Source	Elsevier; Medline; PMC
abstract	BACKGROUND: Given the unceasing worldwide surge in COVID-19 cases, there is an imperative need to develop highly specific and sensitive serology assays to define exposure to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). METHODS: Pooled plasma samples from PCR positive COVID-19 patients were used to identify linear B-cell epitopes from a SARS-CoV-2 peptide library of spike (S), envelope (E), membrane (M), and nucleocapsid (N) structural proteins by peptide-based ELISA. Hit epitopes were further validated with 79 COVID-19 patients with different disease severity status, 13 seasonal human CoV, 20 recovered SARS patients and 22 healthy donors. FINDINGS: Four immunodominant epitopes, S14P5, S20P2, S21P2 and N4P5, were identified on the S and N viral proteins. IgG responses to all identified epitopes displayed a strong detection profile, with N4P5 achieving the highest level of specificity (100%) and sensitivity (>96%) against SARS-CoV-2. Furthermore, the magnitude of IgG responses to S14P5, S21P2 and N4P5 were strongly associated with disease severity. INTERPRETATION: IgG responses to the peptide epitopes can serve as useful indicators for the degree of immunopathology in COVID-19 patients, and function as higly specific and sensitive sero-immunosurveillance tools for recent or past SARS-CoV-2 infections. The flexibility of these epitopes to be used alone or in combination will allow for the development of improved point-of-care-tests (POCTs). FUNDING: Biomedical Research Council (BMRC), the Accelerate GAP-funded project (ACCL/19-GAP064-R20H-H) from Agency of Science, Technology and Research (ASTAR), and National Medical Research Council (NMRC) COVID-19 Research fund (COVID19RF-001) and CCGSFPOR20002. ATR is supported by the Singapore International Graduate Award (SINGA), A*STAR.
has issue date	2020-07-22(xsd:dateTime)
bibo:doi	10.1016/j.ebiom.2020.102911
bibo:pmid	32711254
has license	no-cc
sha1sum (hex)	806750509f3de3786cbd3d991acdfcebe34e6571
schema:url	https://doi.org/10.1016/j.ebiom.2020.102911
resource representing a document's title	Linear B-cell epitopes in the spike and nucleocapsid proteins as markers of SARS-CoV-2 exposure and disease severity
has PubMed Central identifier	PMC7375792
has PubMed identifier	32711254
schema:publication	EBioMedicine
resource representing a document's body	covid:806750509f3de3786cbd3d991acdfcebe34e6571#body_text
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