About: Development of an SPE–HPLC–MS method for simultaneous determination and pharmacokinetic study of bioactive constituents of Yu Ping Feng San in rat plasma after oral administration

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About: Development of an SPE–HPLC–MS method for simultaneous determination and pharmacokinetic study of bioactive constituents of Yu Ping Feng San in rat plasma after oral administration Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Development of an SPE–HPLC–MS method for simultaneous determination and pharmacokinetic study of bioactive constituents of Yu Ping Feng San in rat plasma after oral administration
Creator	Chen, Li Li, Tao Liang, Rixin Wang, Yanli Wang, Yiwei Yang, Weipeng Zhang, Dong Zhang, Huihui
source	Elsevier; Medline; PMC
abstract	Abstract Ethnopharmacological relevance Yu Ping Feng San (YPFS, in Chinese: Jade Windscreen Powder), a well-known traditional Chinese medicine, is commonly used to cure the diseases of respiratory systems and immune systems. Aim of the study A selective and sensitive high-performance liquid chromatography coupled with mass spectrometry method (HPLC–MS) was developed and validated for simultaneous quantification of cycloastragenol, formononetin, calycosin, 4′-O-β-glucopyranosyl-5-O-methylvisamminol (GMV) and cimifugin in rat plasma after oral administration of Yu Ping Feng San decoction. Materials and methods Plasma samples were extracted via solid-phase extraction (SPE), separated on a Zorbax SB-C18 column, detected by single quadruple mass spectrometry with an electrospray ionization interface, and quantified using selected ion monitoring mode. The current SPE–HPLC–MS assay was validated for linearity, intra-day and inter-day precisions, accuracy, extraction recovery and stability. The method was applied to a comparative pharmacokinetic study after administration of Yu Ping Feng San to rats at different doses (10, 20 and 40g/kg). Results The calibration curves were linear over the ranges 0.50–50ng/mL and 17.36–1736ng/mL. Intra- and inter-day precisions (relative standard deviations) were from 0.45% to 10.95%, and accuracy (relative recovery) from 95% to 115%. The extraction recoveries were greater than 88.42% for all analytes. Dose-dependence was shown for some constituents in the drug concentration–time profiles. Among all the active ingredients detected, cimifugin had the highest blood concentration (881–1510ng/mL), and cycloastragenol had the longest retention time in the rat body (15.06–20.44h). Conclusion This analytical method is a selective, sensitive, precise, accurate, and reliable assay for simultaneous determination of cycloastragenol, calycosin, formononetin, GMV, and cimifugin in rat plasma.
has issue date	2013-02-13(xsd:dateTime)
bibo:doi	10.1016/j.jep.2012.12.010
bibo:pmid	23261488
has license	els-covid
sha1sum (hex)	80d48ddb6b4516ab797e00caac070c1d3704343e
schema:url	https://doi.org/10.1016/j.jep.2012.12.010
resource representing a document's title	Development of an SPE–HPLC–MS method for simultaneous determination and pharmacokinetic study of bioactive constituents of Yu Ping Feng San in rat plasma after oral administration
has PubMed Central identifier	PMC7126458
has PubMed identifier	23261488
schema:publication	Journal of Ethnopharmacology
resource representing a document's body	covid:80d48ddb6b4516ab797e00caac070c1d3704343e#body_text
is schema:about of	named entity 'RAT' named entity 'COMPARATIVE' named entity 'DEVELOPMENT' named entity '5-O-METHYLVISAMMINOL' named entity 'RESULTS' named entity 'SINGLE' named entity 'ORAL ADMINISTRATION' named entity 'DEVIATIONS' named entity 'BLOOD CONCENTRATION' named entity 'USED' named entity 'AIM' named entity 'SOLID-PHASE EXTRACTION' named entity 'GREATER THAN' named entity '28881' named entity 'C18 COLUMN' named entity 'ANALYTICAL METHOD' named entity 'ASSAY' named entity 'CURRENT' named entity 'RESPIRATORY SYSTEMS' named entity 'HIGHEST' named entity 'SIMULTANEOUS' named entity 'RELIABLE' named entity 'SENSITIVE' named entity 'DOSE' covid:arg/80d48ddb6b4516ab797e00caac070c1d3704343e named entity 'HPLC-MS' named entity 'PRECISE' named entity 'CIMIFUGIN' named entity 'DIFFERENT' named entity 'DAY' named entity 'STUDY' named entity 'USING' named entity 'CALYCOSIN' named entity 'ACCURACY' named entity 'RETENTION TIME' named entity 'YU PING FENG SAN' named entity 'RECOVERY' named entity 'DISEASES' named entity 'SEPARATED' named entity 'INTERFACE' named entity 'CHINESE' named entity 'HPLC-MS' named entity 'SIMULTANEOUS' named entity 'PHARMACOKINETIC STUDY' named entity 'PLASMA' named entity 'EXTRACTION' named entity 'ACTIVE INGREDIENTS' named entity 'SPE' named entity 'METHOD' named entity 'DETERMINATION' named entity 'CONSTITUENTS' named entity 'YU PING FENG SAN' named entity 'PHARMACOKINETIC STUDY' named entity 'TIME' named entity 'ANALYTES' named entity 'ORAL ADMINISTRATION' named entity 'MODE' named entity 'IMMUNE SYSTEMS' named entity 'LINEAR' named entity 'INTER-' named entity 'PLASMA' named entity 'ACCURATE' named entity 'DECOCTION' named entity 'MASS SPECTROMETRY' named entity '1510' named entity 'TO 115' named entity 'CONCLUSION' named entity 'COUPLED' named entity 'SELECTIVE' named entity 'LONGEST' named entity 'LIQUID CHROMATOGRAPHY ' named entity 'DRUG CONCENTRATION' named entity '0.45' named entity 'METHOD'

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