About: Development and evaluation of a competitive enzyme-linked immunosorbent assay using a monoclonal antibody for diagnosis of severe fever with thrombocytopenia syndrome virus in bovine sera

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About: Development and evaluation of a competitive enzyme-linked immunosorbent assay using a monoclonal antibody for diagnosis of severe fever with thrombocytopenia syndrome virus in bovine sera Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development and evaluation of a competitive enzyme-linked immunosorbent assay using a monoclonal antibody for diagnosis of severe fever with thrombocytopenia syndrome virus in bovine sera
Creator	Song, Jae-Young Cho, In-Soo Kim, Eun-Ju Lee, Hyojin Shin, Yeun-Kyung Choi, Jeong Lee, Ji
Source	Medline; PMC
abstract	Severe fever with thrombocytopenia syndrome (SFTS) caused by the SFTS virus (SFTSV), a phlebovirus in the family Bunyaviridae, is an emerging tick-borne infectious disease that impacts humans. This disease manifests as a decreased blood cell count and multi-organ failure, with a case-fatality rate of more than 12% in China. Because vaccines or antiviral drugs for the treatment of this disease are not available, monitoring the SFTS circulation in animals and controlling the tick-mammal cycle are important for preventing SFTS. Monoclonal antibodies against the recombinant nucleoprotein of SFTSV were generated to develop a competitive enzyme-linked immunosorbent assay (cELISA) for the detection of antibodies against SFTSV infection in cattle. The specificity and sensitivity of cELISA was assessed by comparing the results of this assay to those of an immunofluorescence assay (IFA). The results of the cELISA using 416 field bovine serum samples and laboratory-immunized positive sera showed 98.1% consistency with those of the IFA. The cELISA used in this study did not show cross-reactivity with antisera against other viral cattle diseases. The cELISA presented in this study can be applied to detect antibodies against SFTSV in cattle.
has issue date	2016-09-20(xsd:dateTime)
bibo:doi	10.4142/jvs.2016.17.3.307
bibo:pmid	26435543
has license	cc-by-nc
sha1sum (hex)	80f0bc76680d66fa78c6665807bedcf74b190751
schema:url	https://doi.org/10.4142/jvs.2016.17.3.307
resource representing a document's title	Development and evaluation of a competitive enzyme-linked immunosorbent assay using a monoclonal antibody for diagnosis of severe fever with thrombocytopenia syndrome virus in bovine sera
has PubMed Central identifier	PMC5037297
has PubMed identifier	26435543
schema:publication	J Vet Sci
resource representing a document's body	covid:80f0bc76680d66fa78c6665807bedcf74b190751#body_text
is schema:about of	named entity 'USING' named entity 'RATE' named entity 'TO DETECT' named entity 'PHLEBOVIRUS' named entity 'PREVENTING' named entity 'MAMMAL' named entity 'IMPACTS' named entity 'CHINA' named entity 'MANIFESTS' named entity 'BLOOD CELL COUNT' named entity 'LABORATORY' named entity 'STUDY' named entity 'INFECTION' named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAY' named entity 'SENSITIVITY' named entity 'IMPORTANT' named entity 'MONITORING' named entity 'SEVERE FEVER WITH THROMBOCYTOPENIA SYNDROME' named entity 'DISEASE' named entity 'blood cell count' named entity 'specificity' named entity 'disease' named entity 'consistency' named entity 'monoclonal antibody' named entity 'BOVINE' covid:arg/80f0bc76680d66fa78c6665807bedcf74b190751 named entity 'ENZYME-LINKED IMMUNOSORBENT ASSAY' named entity 'USED' named entity 'APPLIED' named entity 'CELISA' named entity 'RECOMBINANT' named entity 'HUMANS' named entity 'DID' named entity 'SEVERE FEVER WITH THROMBOCYTOPENIA SYNDROME VIRUS' named entity 'FOR DIAGNOSIS' named entity 'SERA' named entity 'COMPETITIVE' named entity 'VACCINES' named entity 'GENERATED' named entity 'FIELD' named entity 'DETECTION' named entity 'CROSS' named entity 'ASSESSED' named entity 'MORE THAN 12' named entity 'MULTI-ORGAN FAILURE' named entity 'CONSISTENCY' named entity 'TICK-BORNE INFECTIOUS DISEASE' named entity 'COMPARING' named entity 'CYCLE' named entity 'CATTLE DISEASES' named entity 'SPECIFICITY' named entity 'SFTS VIRUS' named entity 'VIRAL' named entity 'REACTIVITY' named entity 'SERA' named entity 'DEVELOPMENT' named entity 'CASE' named entity 'IMMUNOFLUORESCENCE ASSAY' named entity 'COMPETITIVE' named entity 'RESULTS' named entity 'CIRCULATION' named entity 'IFA' named entity 'USING' named entity 'ANTIVIRAL DRUGS' named entity 'MONOCLONAL ANTIBODY' named entity 'EVALUATION' named entity 'ASSAY' named entity 'FAMILY BUNYAVIRIDAE' named entity 'ANTIBODIES' named entity 'CATTLE' named entity 'CAUSED BY' named entity 'TREATMENT' named entity 'CONTROLLING' named entity 'IMMUNIZED' named entity 'ANIMALS'

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