About: Microarray analysis reveals the inhibition of intestinal expression of nutrient transporters in piglets infected with porcine epidemic diarrhea virus

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About: Microarray analysis reveals the inhibition of intestinal expression of nutrient transporters in piglets infected with porcine epidemic diarrhea virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Microarray analysis reveals the inhibition of intestinal expression of nutrient transporters in piglets infected with porcine epidemic diarrhea virus
Creator	Li, Peng Zhou, Jia Zhang, Junmei Chen, Hongbo Wu, Tao Zhao, Di Wu, Guoyao Hou, Yongqing Wu, Mengjun Yi, Dan
Source	PMC
abstract	Porcine epidemic diarrhea virus (PEDV) infection can induce intestinal dysfunction, resulting in severe diarrhea and even death, but the mode of action underlying these viral effects remains unclear. This study determined the effects of PEDV infection on intestinal absorption and the expression of genes for nutrient transporters via biochemical tests and microarray analysis. Sixteen 7-day-old healthy piglets fed a milk replacer were randomly allocated to one of two groups. After 5-day adaption, piglets (n = 8/group) were orally administrated with either sterile saline or PEDV (the strain from Yunnan province) at 10(4.5) TCID(50) (50% tissue culture infectious dose) per pig. All pigs were orally infused D-xylose (0.1 g/kg BW) on day 5 post PEDV or saline administration. One hour later, jugular vein blood samples as well as intestinal samples were collected for further analysis. In comparison with the control group, PEDV infection increased diarrhea incidence, blood diamine oxidase activity, and iFABP level, while reducing growth and plasma D-xylose concentration in piglets. Moreover, PEDV infection altered plasma and jejunal amino acid profiles, and decreased the expression of aquaporins and amino acid transporters (L-type amino acid transporter 1, sodium-independent amino acid transporter, B(°(,+))-type amino acid transport protein, sodium-dependent neutral amino acid transporter 1, sodium-dependent glutamate/aspartate transporter 3, and peptide transporter (1), lipid transport and metabolism-related genes (lipoprotein lipase, apolipoprotein A1, apolipoprotein A4, apolipoprotein C2, solute carrier family 27 member 2, solute carrier family 27 member 4, fatty acid synthase, and long-chain acyl-CoA synthetase (3), and glucose transport genes (glucose transporter-2 and insulin receptor) in the jejunum. However, PEDV administration increased mRNA levels for phosphoenolpyruvate carboxykinase 1, argininosuccinate synthase 1, sodium/glucose co-transporter-1, and cystic fibrosis transmembrane conductance regulator in the jejunum. Collectively, these comprehensive results indicate that PEDV infection induces intestinal injury and inhibits the expression of genes encoding for nutrient transporters.
has issue date	2019-12-24(xsd:dateTime)
bibo:doi	10.1038/s41598-019-56391-1
bibo:pmid	31875021
has license	cc-by
sha1sum (hex)	8195b090a2e8333fd41e2d71743d4c316bf69d23
schema:url	https://doi.org/10.1038/s41598-019-56391-1
resource representing a document's title	Microarray analysis reveals the inhibition of intestinal expression of nutrient transporters in piglets infected with porcine epidemic diarrhea virus
has PubMed Central identifier	PMC6930262
has PubMed identifier	31875021
schema:publication	Sci Rep
resource representing a document's body	covid:8195b090a2e8333fd41e2d71743d4c316bf69d23#body_text
is schema:about of	named entity 'apolipoprotein' named entity 'infection' named entity 'strain' named entity 'All' named entity 'genes' named entity 'INSULIN RECEPTOR' named entity 'ENCODING' named entity 'ALTERED' named entity 'FATTY ACID SYNTHASE' named entity 'JEJUNUM' named entity 'MRNA' named entity 'AT 10' named entity 'DECREASED' named entity 'HEALTHY' named entity 'JUGULAR VEIN' named entity 'DIAMINE OXIDASE ACTIVITY' named entity 'INTESTINAL INJURY' named entity 'STERILE' named entity 'TRANSPORTER' named entity 'BLOOD SAMPLES' named entity 'AMINO ACID' named entity 'LONG-CHAIN ACYL-COA SYNTHETASE' named entity 'PHOSPHOENOLPYRUVATE CARBOXYKINASE 1' named entity 'CONCENTRATION' named entity 'ADMINISTRATION' named entity 'TYPE' named entity 'acyl-CoA' named entity 'solute carrier family' named entity 'genes' named entity 'infection' named entity 'Pig' named entity 'small intestine' named entity 'gene expression' named entity 'liquid nitrogen' named entity 'chloride' named entity 'calcium channel' named entity 'cholera toxin' named entity 'mRNA' named entity 'glucose transporters' named entity 'gastrointestinal disease' named entity 'Ingenuity Systems' named entity 'infection' named entity 'plasma' named entity 'citrulline' named entity 'PepT1' named entity 'intron' named entity 'milk replacer' named entity 'lipid' named entity 'nitric oxide' named entity 'GAPDH' named entity 'metabolism of fatty acids' named entity 'proteomic analysis' named entity 'ion' named entity 'metabolized' named entity 'proline' named entity 'hepatitis C virus' named entity 'intracellular' named entity 'Animal Welfare' named entity 'methionine' named entity 'cystine' named entity 'Affymetrix' named entity 'jejunum' named entity 'amino acid transporter' named entity 'Wayne, PA' named entity 'FABP2' named entity 'LDL' named entity 'HDL' named entity 'enzyme' named entity 'mucosal' named entity 'ileal' named entity 'amino acid metabolism' named entity 'ARG1'

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