About: Heat Shock Protein Member 8 Is an Attachment Factor for Infectious Bronchitis Virus

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About: Heat Shock Protein Member 8 Is an Attachment Factor for Infectious Bronchitis Virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Heat Shock Protein Member 8 Is an Attachment Factor for Infectious Bronchitis Virus
Creator	Takano, Tomomi Zhou, Jiyong Yan, Yan Liao, Min Adachi, Akio Gallardo, Rodrigo Xiao, Peng Fang, Chengxiu Lv, Chenfei Peng, Xing Sheng, Hao Zhu, Pengpeng Lin, Vicky Ojha, Kumar Santen, Van
Source	Medline; PMC
abstract	Although infectious bronchitis virus (IBV) is the first coronavirus identified, little is known about which membrane protein of host cells could interact with IBV spike protein and facilitate the infection by the virus. In this study, by using a monoclonal antibody to the S1 protein of IBV M41 strain, we found that heat shock protein member 8 (HSPA8) could interact with spike protein of IBV. HSPA8 was found to be present on the cell membrane and chicken tissues, with highest expression level in the kidney. Results of co-IP and GST-pull-down assays indicated that the receptor binding domain (RBD) of IBV M41 could interact with HSPA8. The results of binding blocking assay and infection inhibition assay showed that recombinant protein HSPA8 and antibody to HSPA8 could inhibit IBV M41 infection of chicken embryonic kidney (CEK) cells. Further, we found that HSPA8 interacted with the N-terminal 19–272 amino acids of S1 of IBV Beaudette, H120 and QX strains and HSPA8 from human and pig also interacted with IBV M41-RBD. Finally the results of binding blocking assay and infection inhibition assay showed that recombinant HSPA8 protein and antibody to HSPA8 could inhibit IBV Beaudette strain infection of Vero cells that were treated with heparanase to remove heparan sulfate from the cell surface. Taken together, our results indicate that HSPA8 is a novel host factor involved in IBV infection.
has issue date	2020-07-10(xsd:dateTime)
bibo:doi	10.3389/fmicb.2020.01630
bibo:pmid	32765462
has license	cc-by
sha1sum (hex)	821e4e6b2e2925f1c774f0fbf32beddd70509689
schema:url	https://doi.org/10.3389/fmicb.2020.01630
resource representing a document's title	Heat Shock Protein Member 8 Is an Attachment Factor for Infectious Bronchitis Virus
has PubMed Central identifier	PMC7381282
has PubMed identifier	32765462
schema:publication	Front Microbiol
resource representing a document's body	covid:821e4e6b2e2925f1c774f0fbf32beddd70509689#body_text
is schema:about of	named entity 'HSPA8' named entity 'HSPA8' named entity 'inhibition' named entity 'virus' named entity 'embryonic' named entity 'Protein' named entity 'level' named entity 'IBV' named entity 'HSPA8' named entity 'M41' named entity 'Results' named entity 'IBV' named entity 'infection' named entity 'protein' named entity 'strain' named entity 'recombinant' named entity 'protein' named entity 'blocking' named entity 'interact' named entity 'protein' named entity 'Vero' named entity 'HSPA8' named entity 'interact' named entity 'RBD' named entity 'IBV' named entity 'interact' named entity 'HSPA8' named entity 'HSPA8' named entity 'IBV' named entity 'host factor' named entity 'IBV' named entity 'antibody' named entity 'IBV' named entity '0.01' named entity 'South China Agricultural University' named entity 'HSPA8' named entity 'host cells' named entity 'GE Healthcare' named entity 'tracheal' named entity 'glycosylation' named entity 'Vero' named entity 'HSPA8' named entity 'mAb' named entity 'host cells' named entity 'HSPA8' named entity 'HSPA8' named entity 'serotypes' named entity 'chicken' named entity 'heat shock protein' named entity 'membrane proteins' named entity 'immunoprecipitated' named entity 'HSPA8' named entity 'Guerrero' named entity 'IBV' named entity 'GAPDH' named entity 'pig' named entity 'pull-down assay' named entity 'cellular receptor' named entity 'protein' named entity 'titer' named entity 'HSPA8' named entity 'anti-flag' named entity 'Spike protein' named entity 'mAb' named entity 'permeabilization' named entity 'spike protein' named entity 'Vero'

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