About: Attenuation and characterization of porcine enteric alphacoronavirus strain GDS04 via serial cell passage

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About: Attenuation and characterization of porcine enteric alphacoronavirus strain GDS04 via serial cell passage Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Attenuation and characterization of porcine enteric alphacoronavirus strain GDS04 via serial cell passage
Creator	Cao, Yongchang Xue, Chunyi Liu, Yuan Peng, Peng Xu, Zhichao Zou, Chuangchao Wei, Ying Gong, Lang Lin, Ying Wu, Yanan
Source	Elsevier; Medline; PMC
abstract	Porcine enteric alphacoronavirus (PEAV) is a newly identified swine enteropathogenic coronavirus that causes watery diarrhea in newborn piglets. In this study, an original, highly virulent PEAV strain GDS04 was serially passaged in Vero cells. The virus titers and sizes of syncytia increased gradually with the cell passages. Newborn piglets were orally inoculated with PEAV P15, P67 and P100. Compared with P15 and P67, P100 resulted in only mild clinical signs and intestinal lesions in piglets. The virus shedding in feces and viral antigens in intestinal tract were markedly reduced in P100-inoculated piglets. Importantly, all P100-inoculated newborn piglets survived, indicating that P100 was an attenuated variant. Sequence analysis revealed that the virulent strain GDS04 had four, one, six and eleven amino acid differences in membrane, nucleocapsid, spike and ORF1ab proteins, respectively, from P100. Furthermore, more differences in the predicted three-dimensional structure of S protein between GDS04 and P100 were observed, indicating that these differences might be associated with the pathogenicity of PEAV. Collectively, our research successfully prepared a PEAV attenuated variant which might serve as a live attenuated vaccine candidate against PEAV infection.
has issue date	2019-11-04(xsd:dateTime)
bibo:doi	10.1016/j.vetmic.2019.108489
bibo:pmid	31767069
has license	no-cc
sha1sum (hex)	829d1f27ec77edc8dfd2ec11c800e0621e9864a0
schema:url	https://doi.org/10.1016/j.vetmic.2019.108489
resource representing a document's title	Attenuation and characterization of porcine enteric alphacoronavirus strain GDS04 via serial cell passage
has PubMed Central identifier	PMC7117411
has PubMed identifier	31767069
schema:publication	Vet Microbiol
resource representing a document's body	covid:829d1f27ec77edc8dfd2ec11c800e0621e9864a0#body_text
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