About: Comparison of expression vectors in Lactobacillus reuteri strains

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Comparison of expression vectors in Lactobacillus reuteri strains
Creator	Cauda, Roberto Lizier, Michela Lucchini, Franco Sarra, Pier
Source	PMC
abstract	The synthesis of heterologous proteins in lactobacilli is strongly influenced by the promoter selected for the expression. In addition, the activity of the promoters themselves may vary among different bacterial hosts. Three different promoters were investigated for their capability to drive enhanced green fluorescent protein (EGFP) expression in Lactococcus lactis spp. cremoris MG1363, in Lactobacillus reuteri DSM 20016(T) and in five L. reuteri strains isolated from chicken crops. The promoters of the Lactobacillus acidophilus surface layer protein gene (slp), L. acidophilus lactate dehydrogenase gene (ldhL) and enterococcal rRNA adenine N-6-methyltransferase gene (ermB) were fused to the coding sequence of EGFP and inserted into the backbone of the pTRKH3 shuttle vector (pTRKH3-slpGFP, pTRKH3-ldhGFP, pTRKH3-ermGFP). Besides conventional analytical methods, a new quick fluorimetric approach was set up to quantify the EGFP fluorescence in transformed clones using the Qubit(™) fluorometer. ermB proved to be the most effective promoter in L. reuteri isolates, producing 3.90 × 10(−7) g of fluorescent EGFP (mL OD(stationary culture))(−1). Under the same conditions, the ldhL promoter produced 2.66 × 10(−7) g of fluorescent EGFP (mL OD(stationary culture))(−1). Even though the slp promoter was efficient in L. lactis spp. cremoris MG1363, it was nearly inactive both in L. reuteri DSM 20016(T) and in L. reuteri isolates.
has issue date	2010-07-01(xsd:dateTime)
bibo:doi	10.1111/j.1574-6968.2010.01978.x
bibo:pmid	20455948
has license	no-cc
sha1sum (hex)	82c3762d19d58438691d589c619b8316a2a07084
schema:url	https://doi.org/10.1111/j.1574-6968.2010.01978.x
resource representing a document's title	Comparison of expression vectors in Lactobacillus reuteri strains
has PubMed Central identifier	PMC7110086
has PubMed identifier	20455948
schema:publication	FEMS Microbiol Lett
resource representing a document's body	covid:82c3762d19d58438691d589c619b8316a2a07084#body_text
is schema:about of	named entity 'isolates' named entity 'addition' named entity 'adenine' named entity 'fluorescent protein' named entity 'heterologous' named entity 'chicken' named entity 'expression' named entity 'erythromycin' named entity 'expression vectors' named entity 'TRANSFORMED' named entity 'ERMB' named entity 'RESISTANT' named entity 'ACTIVITY' named entity 'NEW' named entity 'METHYLTRANSFERASE GENE' named entity 'INACTIVE' named entity 'ISOLATES' named entity 'STRAINS' named entity 'HOSTS' named entity 'INTRODUCED' named entity 'SURFACE LAYER' named entity 'SHUTTLE VECTOR' named entity 'PROTEIN ' named entity 'ISOLATED' named entity 'CULTURE' named entity 'GENE' named entity 'RBS' named entity 'PROMOTER' named entity 'CAPABILITY' named entity 'HETEROLOGOUS' named entity 'ENTEROCOCCAL' named entity 'BACKBONE' named entity 'THEIR' named entity 'LACTOBACILLUS REUTERI' named entity 'PRIMERS' named entity 'EXPRESSION' named entity 'RRNA ADENINE N-6-METHYLTRANSFERASE' named entity 'STATIONARY' named entity 'UP TO' named entity 'NEARLY' named entity 'FUSED TO' named entity 'LACTOBACILLUS ACIDOPHILUS' named entity 'QUICK' named entity 'CLONES' named entity 'CONVENTIONAL' named entity 'CONDITIONS' named entity '20016' named entity 'CHICKEN' named entity 'USING' named entity 'ACIDOPHILUS' named entity 'SYNTHESIS' named entity 'ANALYTICAL METHODS' named entity 'TET' named entity 'TETRACYCLINE' named entity 'DIFFERENT' named entity 'ADDITION' named entity '308' named entity 'FLUORESCENT' named entity 'EXPRESSION VECTORS' named entity 'AMPICILLIN' named entity 'LACTOCOCCUS LACTIS' named entity 'APPROACH' named entity 'EFFECTIVE' named entity 'EGFP' named entity 'COMPARISON' named entity 'STRAINS' named entity 'INVESTIGATED' named entity 'LACTOBACILLUS REUTERI DSM 20016' named entity 'QUANTIFY' named entity 'PRODUCING' named entity 'CROPS' named entity 'FLUORESCENCE' named entity 'LACTATE DEHYDROGENASE' named entity 'DRIVE' named entity 'ERYTHROMYCIN' named entity 'BOLD' named entity 'DSM' named entity 'PROMOTERS'

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