About: Chicken single-chain variable fragments against the SARS-CoV spike protein

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About: Chicken single-chain variable fragments against the SARS-CoV spike protein Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Chicken single-chain variable fragments against the SARS-CoV spike protein
Creator	Lee, Yu-Ching Leu, Sy-Jye Yang, Yi-Yuan Chiu, Wen-Ta Chiang, Bor-Luen Hsieh, Wen-Shyang Wu, Hsueh-Hsia Huang, I-Jen Shih, Neng-Yao Hu, Chaur-Jong
source	Elsevier; Medline; PMC
abstract	The major concern for severe acute respiratory syndrome (SARS), caused by the SARS-associated coronavirus (SARS-CoV), is the lack of diagnostic and therapeutic agents. Using a phage display technology in a chicken system, high-affinity monoclonal antibody fragments against the SARS-CoV spike protein were characterized. Ten truncated spike protein gene fragments were expressed in Escherichia coli cells. Following the immunization of chickens with these recombinant spike proteins, two single-chain variable fragment (scFv) antibody libraries were established with short or long linkers to contain 5 × 10(7) and 9 × 10(6) transformants, respectively. After four rounds of panning selection, the scFv antibodies of randomly chosen clones were demonstrated by Coomassie blue staining, and verified by western blot analysis. In a comparison of nucleotide sequences with the chicken germline gene, we found that all clones varied in the complementarity-determining regions, that two scFv antibodies reacted significantly with SARS-CoV-infected Vero cells, and that those two specific scFv antibodies recognized the same region of the spike protein spanning amino acid residues 750–1000. In conclusion, the results suggest that the chicken scFv phage display system can be a potential model for mass production of high-affinity antibodies against the SARS-CoV spike protein.
has issue date	2007-07-23(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2007.06.010
bibo:pmid	17643500
has license	no-cc
sha1sum (hex)	8371c4ae4f9cbef91435306cd370871ed0b462e4
schema:url	https://doi.org/10.1016/j.jviromet.2007.06.010
resource representing a document's title	Chicken single-chain variable fragments against the SARS-CoV spike protein
has PubMed Central identifier	PMC7112778
has PubMed identifier	17643500
schema:publication	J Virol Methods
resource representing a document's body	covid:8371c4ae4f9cbef91435306cd370871ed0b462e4#body_text
is schema:about of	named entity 'CHICKEN' named entity 'acid' named entity 'chicken' named entity 'varied' named entity 'protein' named entity 'phage display' named entity 'verified' named entity 'spanning' named entity 'clones' named entity 'Chicken' named entity 'SARS-CoV' named entity 'SPIKE PROTEIN' named entity 'FRAGMENTS' named entity 'VARIABLE' named entity 'SINGLE' named entity 'POTENTIAL' named entity 'CONCERN' named entity 'CLONES' named entity 'DEMONSTRATED' named entity 'CHARACTERIZED' named entity 'HIGH' named entity 'PROTEIN ' named entity 'SPIKE PROTEIN' named entity 'PHAGE DISPLAY' named entity 'LIBRARIES' named entity 'REGIONS' named entity '1000' named entity 'PANNING' named entity 'INFECTED' named entity 'CAUSED BY' covid:arg/8371c4ae4f9cbef91435306cd370871ed0b462e4 named entity 'THERAPEUTIC AGENTS' named entity 'ESTABLISHED' named entity 'COMPARISON' named entity 'MASS PRODUCTION' named entity 'SARS-COV' named entity 'SYSTEM' named entity 'REGION' named entity 'CELLS' named entity 'FOUND' named entity 'SELECTION' named entity 'GERMLINE' named entity 'SCFV' named entity 'CONCLUSION' named entity 'IMMUNIZATION' named entity 'ESCHERICHIA COLI' named entity 'SARS-COV' named entity 'CHAIN' named entity 'SHORT' named entity 'LONG' named entity 'DIAGNOSTIC' named entity 'TO CONTAIN' named entity 'RECOMBINANT' named entity 'CHICKENS' named entity 'COOMASSIE BLUE STAINING' named entity 'NUCLEOTIDE SEQUENCES' named entity 'AMINO ACID' named entity 'FRAGMENTS' named entity 'ANTIBODIES' named entity 'MODEL' named entity 'SINGLE-CHAIN VARIABLE FRAGMENT' named entity 'DISPLAY TECHNOLOGY' named entity 'TRUNCATED' named entity 'CHICKEN' named entity 'PROTEIN GENE' named entity 'SUGGEST' named entity 'MONOCLONAL ANTIBODY' named entity 'EXPRESSED' named entity 'USING' named entity 'PROTEINS' named entity 'ANTIBODY' named entity 'SPIKE'

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