About: A multiplex TaqMan real-time PCR for detection and differentiation of four antigenic types of canine parvovirus in China

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About: A multiplex TaqMan real-time PCR for detection and differentiation of four antigenic types of canine parvovirus in China Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A multiplex TaqMan real-time PCR for detection and differentiation of four antigenic types of canine parvovirus in China
Creator	Cao, Zhigang Cheng, Shipeng Cheng, Yuening Li, Shuang Lin, Peng Sun, Yaru Tong, Mingwei Wang, Gaili Wang, Jianke Yi, Li Yuan, Wanzhe
Source	Elsevier; Medline; PMC
abstract	Abstract Canine parvovirus (CPV) is an important pathogen in domestic dogs, and the original antigenic types CPV-2 and its variants, CPV-2a, 2b and 2c, are prevalent worldwide. A multiplex TaqMan real-time PCR method was developed for the detection and differentiation of four antigenic types of CPV. A set of primers and probes, CPV-305F/CPV-305R and CPV-2-305P (for CPV-2)/CPV-2a-305P (for CPV-2a, 2b and 2c), was able to differentiate CPV-2 and its variants (CPV-2a, 2b and 2c). Another set of primers and probes, CPV-426F/CPV-426R and CPV-2-426P (for CPV-2 and 2a)/CPV-2b-426P (for CPV-2b)/CPV-2c-426P (for CPV-2c), was able to differentiate CPV-2a (2), CPV-2b, and CPV-2c. With these primers and probes, the multiplex TaqMan real-time PCR assay detected effectively and differentiated CPV-2, 2a, 2b and 2c by two separate real-time PCRs. No cross reactivity was observed with canine distemper virus, canine adenovirus, and canine coronavirus. The detection limit of the assay is 101 genome copies/μL for CPV-2, CPV-2a, CPV-2b, and 102 copies/μL for CPV-2c. The multiplex real-time PCR has 100% agreement with DNA sequencing. We provide a sensitive assay that simultaneously detects and differentiate four antigenic types of CPV and the method was also used for quantification of CPVs viral genome.
has issue date	2018-04-30(xsd:dateTime)
bibo:doi	10.1016/j.mcp.2018.02.004
bibo:pmid	29499233
has license	els-covid
sha1sum (hex)	83cb4212f61aafd0484e0f0140a343a29e24eca2
schema:url	https://doi.org/10.1016/j.mcp.2018.02.004
resource representing a document's title	A multiplex TaqMan real-time PCR for detection and differentiation of four antigenic types of canine parvovirus in China
has PubMed Central identifier	PMC7126752
has PubMed identifier	29499233
schema:publication	Molecular and Cellular Probes
resource representing a document's body	covid:83cb4212f61aafd0484e0f0140a343a29e24eca2#body_text
is schema:about of	named entity 'CANINE PARVOVIRUS' named entity 'PROVIDE' named entity 'VARIANTS' named entity 'DETECTION LIMIT' named entity 'SET' named entity 'ORIGINAL' named entity 'SEPARATE' named entity 'BCL' named entity 'REALTIME' named entity 'PCR ASSAY' named entity 'DIFFERENTIATED' named entity 'ASSAY' named entity 'PRIMERS' named entity 'CANINE CORONAVIRUS' named entity 'CPV' named entity 'CPV' named entity 'probes' named entity 'antigenic' named entity 'MULTIPLEX' named entity 'CPV' named entity 'THESE' named entity '100%' named entity 'AGREEMENT' named entity 'DETECTED' named entity 'PATHOGEN' covid:arg/83cb4212f61aafd0484e0f0140a343a29e24eca2 named entity 'TAQMAN' named entity 'PCR' named entity 'REAL-TIME PCR ' named entity 'DETECTION' named entity 'GENOME' named entity 'REAL-TIME PCRS' named entity 'COPIES' named entity 'VIRAL GENOME' named entity 'CANINE DISTEMPER VIRUS' named entity 'IMPORTANT' named entity '282' named entity 'CHINA' named entity 'DIFFERENTIATION' named entity 'TYPES' named entity 'QUANTIFICATION' named entity 'DOMESTIC' named entity 'DIFFERENTIATE' named entity 'DNA SEQUENCING' named entity 'SENSITIVE' named entity 'ITS' named entity 'DOGS' named entity 'OBSERVED' named entity 'PROBES' named entity 'REAL-TIME PCR ' named entity 'DETECTION' named entity 'CANINE PARVOVIRUS' named entity 'CROSS REACTIVITY' named entity 'CANINE ADENOVIRUS' named entity 'SIMULTANEOUSLY' named entity 'TYPES' named entity 'MULTIPLEX' named entity 'USED' named entity 'WORLDWIDE' named entity 'PREVALENT' named entity 'DIFFERENTIATION' named entity 'TAQMAN' named entity 'PCR' named entity 'CPV' named entity 'real-time PCR' named entity 'probes' named entity 'adenovirus' named entity 'assay' named entity 'differentiated' named entity 'CPV' named entity 'separate'

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