About: The use of hepatitis C virus NS3/4A and secreted alkaline phosphatase to quantitate cell–cell membrane fusion mediated by severe acute respiratory syndrome coronavirus S protein and the receptor angiotensin-converting enzyme 2

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About: The use of hepatitis C virus NS3/4A and secreted alkaline phosphatase to quantitate cell–cell membrane fusion mediated by severe acute respiratory syndrome coronavirus S protein and the receptor angiotensin-converting enzyme 2 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The use of hepatitis C virus NS3/4A and secreted alkaline phosphatase to quantitate cell–cell membrane fusion mediated by severe acute respiratory syndrome coronavirus S protein and the receptor angiotensin-converting enzyme 2
Creator	Tan, Yee-Joo Chou, Chih-Fong Hong, Wanjin Shen, Shuo Mahadevappa, Geetha Lim, Gee
Source	Elsevier; Medline; PMC
abstract	Abstract The membrane fusion process mediated by severe acute respiratory syndrome coronavirus (SARS–CoV) S protein and its cellular receptor angiotensin-converting enzyme 2 (ACE2) had been reconstituted using two Chinese hamster ovary (CHO) cell lines that constitutively express these recombinant proteins separately. This system was applied to develop a quantitative measurement of cell–cell fusion using hepatitis C virus (HCV) NS3/4A protease and a secretion-blocked EGFP-4A/4B-SEAP (EGFP: enhanced green fluorescent protein; 4A/4B: a decapeptide substrate of NS3/4A protease; SEAP: secreted alkaline phosphatase) fusion gene. Both genes were transiently expressed in either of the CHO cell lines, followed by fusion treatment. Significant SEAP activity could be detected in the culture medium only after cell–cell fusion occurred. Cell–cell fusion provides an environment in which the protease encounters its substrate 4A/4B, thereby releasing SEAP from the fusion protein. In this study, we developed a simple, sensitive, and quantitative assay to study the membrane fusion process by measuring the extracellular activity of SEAP.
has issue date	2007-07-15(xsd:dateTime)
bibo:doi	10.1016/j.ab.2007.04.031
bibo:pmid	17553448
has license	els-covid
sha1sum (hex)	8421dfdeccb793a0f326dede0a11e437450b6b59
schema:url	https://doi.org/10.1016/j.ab.2007.04.031
resource representing a document's title	The use of hepatitis C virus NS3/4A and secreted alkaline phosphatase to quantitate cell–cell membrane fusion mediated by severe acute respiratory syndrome coronavirus S protein and the receptor angiotensin-converting enzyme 2
has PubMed Central identifier	PMC7094373
has PubMed identifier	17553448
schema:publication	Analytical Biochemistry
resource representing a document's body	covid:8421dfdeccb793a0f326dede0a11e437450b6b59#body_text
is schema:about of	named entity 'CHO cell lines' named entity 'RECONSTITUTED' named entity 'QUANTITATIVE' named entity 'ENHANCED GREEN FLUORESCENT PROTEIN' named entity 'EXPRESSED' named entity 'BLOCKED' named entity 'TREATMENT' named entity 'FOLLOWED BY' named entity 'CHO CELL' named entity 'OVARY' named entity 'CELLULAR' named entity 'FUSION PROTEIN' named entity 'SENSITIVE' named entity 'THESE' named entity 'RECEPTOR' named entity 'USING' named entity 'MEDIATED' named entity 'GENES' named entity 'ACTIVITY' named entity 'COULD BE' named entity 'OCCURRED' named entity 'SUBSTRATE' named entity 'ENVIRONMENT' named entity 'MEMBRANE FUSION' named entity 'PROTEIN ' named entity 'CELL-CELL FUSION' named entity 'S PROTEIN' named entity 'PROTEASE' named entity 'ASSAY' named entity 'MEASURING' named entity 'ALKALINE PHOSPHATASE' named entity 'MEASUREMENT' named entity 'SYSTEM' named entity 'ANALYTICAL BIOCHEMISTRY' named entity 'CHINESE HAMSTER' named entity 'SIGNIFICANT' named entity 'STUDY' named entity 'PROCESS' named entity 'FUSION GENE' named entity 'DETECTED' named entity 'EITHER' named entity 'PROVIDES' named entity 'HEPATITIS C VIRUS' named entity 'EGFP' named entity 'SECRETED' named entity 'ITS' named entity 'RECOMBINANT PROTEINS' named entity 'EXPRESS' named entity 'ANGIOTENSIN-CONVERTING ENZYME 2' named entity 'RELEASING' named entity 'CULTURE MEDIUM' named entity 'APPLIED' named entity 'CELL LINES' named entity 'EXTRACELLULAR' named entity 'NS3' named entity 'FUSION' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS' named entity 'SECRETION' named entity 'SIMPLE' covid:arg/8421dfdeccb793a0f326dede0a11e437450b6b59 named entity 'study' named entity 'NS3' named entity 'activity' named entity 'developed' named entity 'recombinant proteins' named entity 'assay' named entity 'fluorescent protein' named entity 'hepatitis C virus' named entity 'cell lines' named entity 'SARS-CoV' named entity 'recombinant proteins' named entity 'assay' named entity 'cell-cell' named entity 'hepatitis C virus' named entity 'secretion' named entity 'fusion gene'

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