About: Construction of plasmids expressing Sars-CoV encoding proteins and their effects on transcription of hfgl2 prothrombinase

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About: Construction of plasmids expressing Sars-CoV encoding proteins and their effects on transcription of hfgl2 prothrombinase Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Construction of plasmids expressing Sars-CoV encoding proteins and their effects on transcription of hfgl2 prothrombinase
Creator	Luo, Xiaoping Ning, Qin Wang, Hongwu Xi, Dong Yan, Weiming Hou, Jinlin Wang, Zhanhui Yao, Huaning
Source	Medline; PMC
abstract	SARS coronavirus (SARS-CoV) is the etiologic agent of severe acute respiratory syndrome. The aim of this study was to construct Sars-CoV membrane (M), nucleocapsid (N) and spike 2 (S2) gene eukaryotic expression plasmids, and identify their expression in vitro. Gene fragments encoding N protein, M protein and S2 protein of SARS-CoV were amplified by PCR using cDNA obtained from lung samples of SARS patients as template, and subcloned into pcDNA3.1 vector to form eukaryotic expression plasmids. SARS-CoV protein eukaryotic expression plasmids were transfected respectively into CHO cells. Immunohistochemistry was employed to detect the expression of the structural proteins of SARS-CoV in transfected cells. SARS-CoV protein eukaryotic expression plasmids were successfully constructed by identification with digestion of restriction enzymes and sequencing. M, N and S2 proteins of SARS-CoV were detected in the cytoplasm of transfected CHO cells. It was concluded that these recombinant eukaryotic expression plasmids were constructed successfully, and SARS-CoV encoding proteins could activate transcription and expression of hfgl2 gene.
has issue date	2009-06-10(xsd:dateTime)
bibo:doi	10.1007/s11596-009-0311-1
bibo:pmid	19513614
has license	no-cc
sha1sum (hex)	8441e6eb8e1af2ae4726e7e786405a3bff5b4a8e
schema:url	https://doi.org/10.1007/s11596-009-0311-1
resource representing a document's title	Construction of plasmids expressing Sars-CoV encoding proteins and their effects on transcription of hfgl2 prothrombinase
has PubMed Central identifier	PMC7089052
has PubMed identifier	19513614
schema:publication	J Huazhong Univ Sci Technolog Med Sci
resource representing a document's body	covid:8441e6eb8e1af2ae4726e7e786405a3bff5b4a8e#body_text
is schema:about of	named entity 'nucleocapsid' named entity 'PCR' named entity 'membrane' named entity 'Sars-CoV' named entity 'protein' named entity 'eukaryotic' named entity 'spike' named entity 'cDNA' named entity 'Sars-CoV' named entity 'PROTEINS' named entity 'IDENTIFY' named entity 'TEMPLATE' named entity 'SUMMARY' named entity 'STRUCTURAL PROTEINS' named entity 'HFGL2' named entity 'ACTIVATE' named entity 'FRAGMENTS' named entity 'VECTOR' named entity 'LUNG' named entity 'IDENTIFICATION' named entity 'PCR' named entity 'ENCODING' named entity 'AMPLIFIED' named entity 'SEQUENCING' named entity 'CDNA' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'CYTOPLASM' named entity 'CHO CELLS' named entity 'ENZYMES' named entity 'NUCLEOCAPSID' named entity 'SPIKE' named entity 'SARS' covid:arg/8441e6eb8e1af2ae4726e7e786405a3bff5b4a8e named entity 'PROTHROMBINASE' named entity 'DETECTED' named entity 'OBTAINED' named entity 'RESTRICTION' named entity 'USING' named entity 'IMMUNOHISTOCHEMISTRY' named entity 'SARS-COV PROTEIN' named entity 'THESE' named entity 'CORONAVIRUS' named entity 'TO FORM' named entity 'EMPLOYED' named entity 'STUDY' named entity 'ENCODING' named entity 'THEIR' named entity 'TRANSCRIPTION' named entity 'CONSTRUCTION' named entity 'EFFECTS' named entity 'SARS-COV' named entity 'HFGL2' named entity 'SARS-COV' named entity 'GENE' named entity 'AIM' named entity 'IN VITRO' named entity 'THEIR' named entity 'ETIOLOGIC AGENT' named entity 'PROTEINS' named entity 'PATIENTS' named entity 'CELLS' named entity 'EUKARYOTIC' named entity 'CONSTRUCTED' named entity 'EXPRESSION' named entity 'CONSTRUCT' named entity 'M PROTEIN' named entity 'SAMPLES' named entity 'PLASMIDS' named entity 'TRANSCRIPTION' named entity 'PLASMIDS' named entity 'DIGESTION' named entity 'N PROTEIN' named entity 'MEMBRANE' named entity 'PROTEIN '

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