About: Evaluation of Seegene Allplex Respiratory Panel 1 kit for the detection of influenza virus and human respiratory syncytial virus

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About: Evaluation of Seegene Allplex Respiratory Panel 1 kit for the detection of influenza virus and human respiratory syncytial virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Evaluation of Seegene Allplex Respiratory Panel 1 kit for the detection of influenza virus and human respiratory syncytial virus
Creator	Alcubilla, Pilar Andrés, Cristina Antón, Andrés Fuentes, Francisco Gimferrer, Laura Piñana, Maria Pumarola, Tomàs Rando, Ariadna Rubio, Susana Del, Maria Codina, Maria Martin, Carmen
source	Elsevier; Medline; PMC
abstract	BACKGROUND: Influenza (FLUV) and human respiratory syncytial (HRSV) viruses are etiological agents of respiratory infections that cause a significant morbidity and mortality worldwide. A rapid and accurate diagnosis of these respiratory viruses is essential for an appropriate patient management. Molecular tests are the best detection option due to their high sensitivity and specificity. Seegene’s Allplex™ Respiratory Panel 1 (Allplex RP1) is a real-time one-step RT-PCR assay for the simultaneous detection of FLUAV, FLUBV, HRSV-A and HRSV-B. In addition, it allows the determination of FLUAV subtype (H1, H3 and H1pdm09). OBJECTIVES: This study aims to evaluate Allplex RP1 as a rapid molecular test for the detection of FLUAV, FLUBV, HRSV-A and HRSV-B viruses. STUDY DESIGN: The Allplex RP1 assay will be compared with other two commercial molecular assays, Prodesse ProFlu+ and ProFAST+ (Hologic, Madison, WI, USA), and GeneXpert Flu/RSV XC (Cepheid, USA). RESULTS: Allplex RP1, ProFlu+ and GeneXpert tests showed 95%, 91% and 96% of accuracy; and 94%, 88% and 95% of sensitivity, respectively. Moreover, Allplex RP1 showed a FLUAV subtype sensitivity of 91% and 88% for FLUAV-H1pdm09 and FLUAV-H3 respectively, and ProFAST+ assay showed sensitivities of 100% for both targets. The three assays showed a 100% of specificity and PPV, while the NPV were 84%, 73% and 86% for Allplex RP1, Prodesse and GeneXpert, respectively. CONCLUSIONS: In this study, Seegene’s Allplex RP1 assay showed to be highly sensitive, specific, and suitable for detection of FLUV and HRSV, including FLUAV subtyping. In addition, it is also a hands-on-time saving assay due to the automated nucleic acid extraction and PCR setup.
has issue date	2018-05-25(xsd:dateTime)
bibo:doi	10.1016/j.jcv.2018.05.006
bibo:pmid	29883908
has license	no-cc
sha1sum (hex)	84564b1c4576582020f42d649003950aaf9f55ca
schema:url	https://doi.org/10.1016/j.jcv.2018.05.006
resource representing a document's title	Evaluation of Seegene Allplex Respiratory Panel 1 kit for the detection of influenza virus and human respiratory syncytial virus
has PubMed Central identifier	PMC7106510
has PubMed identifier	29883908
schema:publication	J Clin Virol
resource representing a document's body	covid:84564b1c4576582020f42d649003950aaf9f55ca#body_text
is schema:about of	named entity 'RP1' named entity 'patient' named entity 'RT-PCR' named entity 'human respiratory syncytial virus' named entity 'RESPIRATORY' named entity 'DETECTION' named entity 'DETECTION' named entity 'FLUBV' named entity '27S' named entity 'WORLDWIDE' named entity 'MOLECULAR' named entity 'HUMAN' named entity 'RP1' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'OPTION' named entity 'SIMULTANEOUS' named entity 'HRSV-A' named entity 'RESPIRATORY' named entity 'HUMAN RESPIRATORY SYNCYTIAL VIRUS' covid:arg/84564b1c4576582020f42d649003950aaf9f55ca named entity 'PANEL' named entity 'SUBTYPE' named entity 'SIGNIFICANT' named entity 'RESPIRATORY INFECTIONS' named entity 'INFLUENZA' named entity 'PANEL' named entity 'KIT' named entity 'EVALUATION' named entity 'DUE TO' named entity 'APPROPRIATE' named entity 'CAUSE' named entity 'RT-PCR ASSAY' named entity 'PATIENT MANAGEMENT' named entity 'VIRUSES' named entity 'MORBIDITY AND MORTALITY' named entity 'THEIR' named entity 'FLUAV' named entity 'DIAGNOSIS' named entity 'ALLOWS' named entity 'DETERMINATION' named entity 'SYNCYTIAL' named entity 'STEP' named entity 'IS A' named entity 'TESTS' named entity 'BACKGROUND' named entity 'BEST' named entity 'RAPID' named entity 'ESSENTIAL' named entity 'THESE' named entity 'RESPIRATORY VIRUSES' named entity 'HRSV-B' named entity 'INFLUENZA VIRUS' named entity 'ACCURATE' named entity 'HIGH SENSITIVITY' named entity 'REAL-TIME' named entity 'ADDITION' named entity 'respiratory' named entity 'Molecular' named entity 'Influenza' named entity 'agents' named entity 'sensitivity' named entity 'real-time' named entity 'Evaluation' named entity 'Influenza' named entity 'HRSV' named entity 'viruses' named entity 'influenza virus' named entity 'PCR-based methods' named entity 'HRSV' named entity 'viruses'

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