About: High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

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About: High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
Creator	Ortenberg, Elen Slater, Marcia Dubovi, Edward Dubovi, J Goodman, Laura Anderson, L Anderson, Renee Beeby, John Chilson, Brittany Chilson, R Glaser, Amy Glaser, E Goodman, Citation High, A Laverack, Melissa Renshaw, E Renshaw, Randall Slater, R
topic	covid:845be0716daab542bf27a9d735e03f86a8f3ea85#this
Source	Medline; PMC
abstract	Nanoliter scale real-time PCR uses spatial multiplexing to allow multiple assays to be run in parallel on a single plate without the typical drawbacks of combining reactions together. We designed and evaluated a panel based on this principle to rapidly identify the presence of common disease agents in dogs and horses with acute respiratory illness. This manuscript describes a nanoscale diagnostic PCR workflow for sample preparation, amplification, and analysis of target pathogen sequences, focusing on procedures that are different from microliter scale reactions. In the respiratory panel presented, 18 assays were each set up in triplicate, accommodating up to 48 samples per plate. A universal extraction and pre-amplification workflow was optimized for high-throughput sample preparation to accommodate multiple matrices and DNA and RNA based pathogens. Representative data are presented for one RNA target (influenza A matrix) and one DNA target (equine herpesvirus 1). The ability to quickly and accurately test for a comprehensive, syndrome-based group of pathogens is a valuable tool for improving efficiency and ergonomics of diagnostic testing and for acute respiratory disease diagnosis and management.
has issue date	2016-11-28(xsd:dateTime)
bibo:doi	10.3791/54781
bibo:pmid	27929456
has license	cc-by-nc-nd
sha1sum (hex)	845be0716daab542bf27a9d735e03f86a8f3ea85
schema:url	https://doi.org/10.3791/54781
resource representing a document's title	High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
has PubMed Central identifier	PMC5226323
has PubMed identifier	27929456
schema:publication	J Vis Exp
resource representing a document's body	covid:845be0716daab542bf27a9d735e03f86a8f3ea85#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/845be0716daab542bf27a9d735e03f86a8f3ea85
is schema:about of	named entity 'MATRIX' named entity 'ALLOW' named entity 'SAMPLE PREPARATION' named entity 'SEQUENCES' named entity 'PLATE' named entity 'RAPIDLY' named entity 'PARALLEL' named entity 'WORKFLOW' named entity 'BASED' named entity 'ACUTE RESPIRATORY DISEASE' named entity 'COMBINING' named entity 'ERGONOMICS' named entity 'MICROLITER' named entity 'PROCEDURES' named entity 'PATHOGENS' named entity 'PRINCIPLE' named entity 'PRESENTED' named entity 'MATRICES' named entity 'HIGH' named entity 'DOGS' named entity 'PATHOGEN' named entity 'DISEASE DIAGNOSIS' named entity 'TEST' named entity 'REPRESENTATIVE' named entity 'SAMPLES' named entity 'THROUGHPUT' named entity 'IMPROVING' named entity 'ANALYSIS' named entity 'FOCUSING' named entity 'sample preparation' named entity 'principle' named entity 'disease' named entity 'optimized' named entity 'triplicate' named entity 'workflow' named entity 'typical' named entity 'ABILITY TO' named entity 'NANOSCALE' named entity 'PCR' named entity 'IDENTIFY' named entity 'SYNDROME' covid:arg/845be0716daab542bf27a9d735e03f86a8f3ea85 named entity 'DETECTION' named entity 'HERPESVIRUS' named entity 'DNA' named entity 'DIAGNOSTIC PCR' named entity 'GROUP' named entity 'PANEL' named entity 'SPATIAL' named entity 'USES' named entity 'COMMON DISEASE' named entity 'EVALUATED' named entity 'TOOL' named entity 'SINGLE' named entity 'SPECIMENS' named entity 'RNA' named entity 'MANUSCRIPT' named entity 'AMPLIFICATION' named entity 'REACTIONS' named entity 'IS A' named entity 'NANOSCALE' named entity 'NANOLITER'

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