About: Development of a real-time loop-mediated isothermal amplification assay for detection of porcine circovirus 3

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About: Development of a real-time loop-mediated isothermal amplification assay for detection of porcine circovirus 3 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of a real-time loop-mediated isothermal amplification assay for detection of porcine circovirus 3
Creator	Cong, Feng Huang, Ren Zhang, Yu Chen, Meili Guo, Pengju Lian, Yuexiao Liu, Xiangnan Luo, Manlin Wang, Huanan Wu, Miaoli Xiao, Li Zeng, Fanwen Zhang, Tongyuan Zhu, Yujun
Source	PMC
abstract	BACKGROUND: Porcine circovirus type 3 (PCV3) is an emerging circovirus species, that has been reported in major pig-raising countries including the United States, China, South Korea, Brazil, Spain, and Poland. RESULTS: A real-time loop-mediated isothermal amplification (LAMP) assay was developed for rapid detection of porcine circovirus 3 (PCV3). The method had a detection limit of 1 × 10(1) copies/μL with no cross-reactions with classical swine fever virus (CSFV) C strain, foot-and-mouth disease virus (FMDV), porcine circovirus 2 (PCV2) LG vaccine strain, porcine epidemic diarrhoea virus (PEDV), porcine respiratory and reproductive syndrome virus (PRRSV), or pseudorabies virus (PRV). The PCV3 positive detection rate of 203 clinical samples for the real-time LAMP assay was 89.66% (182/203). CONCLUSIONS: The real-time LAMP assay is highly sensitive, and specific for use in epidemiological investigations of PCV3.
has issue date	2019-08-23(xsd:dateTime)
bibo:doi	10.1186/s12917-019-2037-z
bibo:pmid	31443656
has license	cc-by
sha1sum (hex)	850c8a23db306b9410adc341809d40d8b46635aa
schema:url	https://doi.org/10.1186/s12917-019-2037-z
resource representing a document's title	Development of a real-time loop-mediated isothermal amplification assay for detection of porcine circovirus 3
has PubMed Central identifier	PMC6706899
has PubMed identifier	31443656
schema:publication	BMC Vet Res
resource representing a document's body	covid:850c8a23db306b9410adc341809d40d8b46635aa#body_text
is schema:about of	named entity 'DEVELOPMENT' named entity 'type 3' named entity 'Porcine circovirus' named entity 'real-time' named entity 'COUNTRIES' named entity 'PIG-' named entity 'PORCINE CIRCOVIRUS TYPE 3' named entity 'SPECIES' named entity 'BACKGROUND' named entity 'ISOTHERMAL AMPLIFICATION ASSAY' named entity 'REPORTED' named entity 'PORCINE CIRCOVIRUS 3' named entity 'REAL-TIME' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'INCLUDING' named entity 'CIRCOVIRUS' named entity 'DETECTION' named entity 'MAJOR' named entity 'loop-mediated isothermal amplification' named entity 'Beijing' named entity 'Italy' named entity 'real-time quantitative PCR' named entity 'DH5α' named entity 'diagnostic assays' named entity 'PCR' named entity 'porcine epidemic diarrhoea' named entity 'United Kingdom' named entity 'plasmid DNA' named entity 'vector' named entity 'swine' named entity 'United States' named entity 'China' named entity 'virus' named entity 'pathogenesis' named entity 'detection limit' named entity 'porcine circovirus' named entity 'classical swine fever virus' named entity 'papules' named entity 'PCV2' named entity 'plasmid' named entity 'PCR' named entity 'virus' named entity 'PCV2' named entity 'porcine' named entity 'detection limit' named entity 'lungs' named entity 'pig' named entity 'nucleic acids' named entity 'Takara' named entity 'historical data' named entity 'pathogens' named entity 'Virus' named entity 'June 2015' named entity 'Beijing' named entity 'China' named entity 'phosphate-buffered saline' named entity 'Spain' named entity 'absorbance' named entity 'Shanghai' named entity 'pseudorabies virus' named entity 'cloned' named entity 'reverse transcription' named entity 'anorexic' named entity 'real-time PCR' named entity 'homogenized' named entity 'DNA and RNA' named entity 'RNA viruses' named entity 'China'

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