About: Comparative analysis of primer-probe sets for the laboratory confirmation of SARS-CoV-2

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About: Comparative analysis of primer-probe sets for the laboratory confirmation of SARS-CoV-2 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Comparative analysis of primer-probe sets for the laboratory confirmation of SARS-CoV-2
Creator	Lee, Jong-Hwan Jung, Yu Kim, Hong Kim, Seung Lee, Joong Lee, Min Kim, Seong Beak, Seung-Hwa Kim, Bum-Tae Kim, Seil Ku, Keunbon Maeng, Jin-Soo Moon, Jun Park, Daeui Park, Edmond Park, Gun-Soo Woo Byeon, Cheol
Source	BioRxiv; MedRxiv
abstract	Coronavirus disease 2019 (COVID-19) is newly emerging human infectious diseases, which is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2, also previously known as 2019-nCoV). Within two months of the outbreak, more than 80,000 cases of COVID-19 have been confirmed worldwide. Since the human to human transmission occurred easily and the human infection is rapidly increasing, the sensitive and early diagnosis is essential to prevent the global outbreak. Recently, World Health Organization (WHO) announced various primer and probe sets for SARS-CoV-2 previously developed in China, Germany, Hong Kong, Japan, Thailand, and USA. In this study, we compared the ability to detect SARS-CoV-2 RNA among the seven primer-probe sets for N gene and the three primer-probe sets for Orf1 gene. The result of the comparative analysis represented that the ‘2019-nCoV_N2, N3’ of USA and the ‘ORF1ab’ of China are the most sensitive primer-probe sets for N and Orf1 genes, respectively. Therefore, the appropriate combination from ORF1ab (China), 2019-nCoV_N2, N3 (USA), and NIID_2019-nCOV_N (Japan) sets should be selected for the sensitive and reliable laboratory confirmation of SARS-CoV-2.
has issue date	2020-02-27(xsd:dateTime)
bibo:doi	10.1101/2020.02.25.964775
has license	biorxiv
sha1sum (hex)	86b6b0c1b2777541feb83116bcb7a5cb12a52310
schema:url	https://doi.org/10.1101/2020.02.25.964775
resource representing a document's title	Comparative analysis of primer-probe sets for the laboratory confirmation of SARS-CoV-2
schema:publication	bioRxiv
resource representing a document's body	covid:86b6b0c1b2777541feb83116bcb7a5cb12a52310#body_text
is schema:about of	named entity 'PRIMER' named entity 'CONFIRMATION OF' named entity 'PROBE' named entity 'SARS-COV-2' named entity 'COMPARATIVE ANALYSIS' named entity 'LABORATORY' named entity 'SARS-CoV-2' named entity 'peer-reviewed' named entity 'Japan' named entity 'severe acute respiratory syndrome' named entity 'SARS-CoV-2' named entity 'culture medium' named entity 'SARS-CoV-2' named entity 'RdRp' named entity 'Japan' named entity 'gene' named entity 'incubation' named entity 'RNA' named entity 'Hilden' named entity 'Pathogen' named entity 'pneumonia' named entity 'Coronavirus' named entity 'COVID' named entity 'SARS-CoV-2' named entity 'preprint' named entity 'standard curve' named entity 'SARS-CoV-2' named entity 'enzyme' named entity 'Thailand' named entity 'qRT-PCR' named entity 'African green monkey' named entity 'COVID' named entity 'bioRxiv' named entity 'RdRp' named entity 'RdRp' named entity 'coronavirus' named entity 'Korea' named entity 'cross-react' named entity 'preprint' named entity 'Coronavirus Disease' named entity 'World Health Organization' named entity 'Invitrogen' named entity 'infection' named entity 'gene' named entity 'ACE2' named entity 'RNA' named entity 'ORF 1' named entity 'molecular diagnosis' named entity 'qRT-PCR' named entity 'SARS-CoV-2' named entity 'qRT-PCR' named entity 'virions' named entity 'virus' named entity 'peer-reviewed' named entity 'Orf1' named entity '23 February 2020' named entity 'CoV' named entity 'Influenza' named entity 'MERS-CoV' named entity 'qRT-PCR' named entity 'Korea' named entity 'protein' named entity 'qRT-PCR' named entity 'bioRxiv' named entity 'viral RNA' named entity 'cough' named entity 'peer-reviewed' named entity 'preprint'

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