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About:
Comparative analysis of primer-probe sets for the laboratory confirmation of SARS-CoV-2
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schema:ScholarlyArticle
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wasabi.inria.fr
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Type:
Academic Article
research paper
schema:ScholarlyArticle
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type
Academic Article
research paper
schema:ScholarlyArticle
isDefinedBy
Covid-on-the-Web dataset
has title
Comparative analysis of primer-probe sets for the laboratory confirmation of SARS-CoV-2
Creator
Lee, Jong-Hwan
Jung, Yu
Kim, Hong
Kim, Seung
Lee, Joong
Lee, Min
Kim, Seong
Beak, Seung-Hwa
Kim, Bum-Tae
Kim, Seil
Ku, Keunbon
Maeng, Jin-Soo
Moon, Jun
Park, Daeui
Park, Edmond
Park, Gun-Soo
Woo Byeon, Cheol
Source
BioRxiv; MedRxiv
abstract
Coronavirus disease 2019 (COVID-19) is newly emerging human infectious diseases, which is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2, also previously known as 2019-nCoV). Within two months of the outbreak, more than 80,000 cases of COVID-19 have been confirmed worldwide. Since the human to human transmission occurred easily and the human infection is rapidly increasing, the sensitive and early diagnosis is essential to prevent the global outbreak. Recently, World Health Organization (WHO) announced various primer and probe sets for SARS-CoV-2 previously developed in China, Germany, Hong Kong, Japan, Thailand, and USA. In this study, we compared the ability to detect SARS-CoV-2 RNA among the seven primer-probe sets for N gene and the three primer-probe sets for Orf1 gene. The result of the comparative analysis represented that the ‘2019-nCoV_N2, N3’ of USA and the ‘ORF1ab’ of China are the most sensitive primer-probe sets for N and Orf1 genes, respectively. Therefore, the appropriate combination from ORF1ab (China), 2019-nCoV_N2, N3 (USA), and NIID_2019-nCOV_N (Japan) sets should be selected for the sensitive and reliable laboratory confirmation of SARS-CoV-2.
has issue date
2020-02-27
(
xsd:dateTime
)
bibo:doi
10.1101/2020.02.25.964775
has license
biorxiv
sha1sum (hex)
86b6b0c1b2777541feb83116bcb7a5cb12a52310
schema:url
https://doi.org/10.1101/2020.02.25.964775
resource representing a document's title
Comparative analysis of primer-probe sets for the laboratory confirmation of SARS-CoV-2
schema:publication
bioRxiv
resource representing a document's body
covid:86b6b0c1b2777541feb83116bcb7a5cb12a52310#body_text
is
schema:about
of
named entity 'PRIMER'
named entity 'CONFIRMATION OF'
named entity 'PROBE'
named entity 'SARS-COV-2'
named entity 'COMPARATIVE ANALYSIS'
named entity 'LABORATORY'
named entity 'SARS-CoV-2'
named entity 'peer-reviewed'
named entity 'Japan'
named entity 'severe acute respiratory syndrome'
named entity 'SARS-CoV-2'
named entity 'culture medium'
named entity 'SARS-CoV-2'
named entity 'RdRp'
named entity 'Japan'
named entity 'gene'
named entity 'incubation'
named entity 'RNA'
named entity 'Hilden'
named entity 'Pathogen'
named entity 'pneumonia'
named entity 'Coronavirus'
named entity 'COVID'
named entity 'SARS-CoV-2'
named entity 'preprint'
named entity 'standard curve'
named entity 'SARS-CoV-2'
named entity 'enzyme'
named entity 'Thailand'
named entity 'qRT-PCR'
named entity 'African green monkey'
named entity 'COVID'
named entity 'bioRxiv'
named entity 'RdRp'
named entity 'RdRp'
named entity 'coronavirus'
named entity 'Korea'
named entity 'cross-react'
named entity 'preprint'
named entity 'Coronavirus Disease'
named entity 'World Health Organization'
named entity 'Invitrogen'
named entity 'infection'
named entity 'gene'
named entity 'ACE2'
named entity 'RNA'
named entity 'ORF 1'
named entity 'molecular diagnosis'
named entity 'qRT-PCR'
named entity 'SARS-CoV-2'
named entity 'qRT-PCR'
named entity 'virions'
named entity 'virus'
named entity 'peer-reviewed'
named entity 'Orf1'
named entity '23 February 2020'
named entity 'CoV'
named entity 'Influenza'
named entity 'MERS-CoV'
named entity 'qRT-PCR'
named entity 'Korea'
named entity 'protein'
named entity 'qRT-PCR'
named entity 'bioRxiv'
named entity 'viral RNA'
named entity 'cough'
named entity 'peer-reviewed'
named entity 'preprint'
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