About: On-chip analysis of respiratory viruses from nasopharyngeal samples

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	On-chip analysis of respiratory viruses from nasopharyngeal samples
Creator	Attig, Hans Claußen, Jan Dahlke, Rainer Großhauser, Gerd Himmelreich, Ralf Holzer, Eva Jeziorski, Markus Ritzi-Lehnert, Marion Schaeffer, Eva Wende, Andy Werner, Sabine Wiborg, Jens Wick, Isabell Drese, Thomas Klaus, Stefan Rothmann,
Source	Medline; PMC
abstract	Point-of-care (PoC) testing followed by personalized efficient therapy of infectious diseases may result in a considerable reduction of associated health care costs. Lab-on-a-chip (LoC) systems represent a potentially high efficient class of PoC tools. Here, we present a LoC system for automated pathogen analysis of respiratory viruses from nasopharyngeal specimens. The device prepares total nucleic acids from extracted swab samples using magnetic silica beads. After reverse transcription the co-purified viral RNA is amplified in accordance with the QIAplex multiplex PCR technology. Hybridized to corresponding QIAGEN LiquiChip beads and labelled with streptavidin R-phycoerythrin, the amplified target sequences are finally detected using a QIAGEN LiquiChip200 workstation. All chemicals needed are either stored freeze-dried on the disposable chip or are provided in liquid form in a reagent cartridge for up to 24 runs. Magnetic stir bars for mixing as well as turning valves with metering structures are integrated into the injection-moulded disposable chip. The core of the controlling instrument is a rotating heating bar construction providing fixed temperatures for fast cycling. PCR times of about half an hour (for 30 cycles) could be achieved for 120 μl reactions, making this system the fastest currently available high-volume PCR chip. The functionality of the system was shown by comparing automatically processed nasopharyngeal samples to ones processed manually according to the QIAGEN “ResPlex™ II Panel v2.0” respiratory virus detection kit. A prototype of the present instrument revealed slightly weaker signal intensities with a similar sensitivity in comparison to the commercially available kit and automated nucleic acid preparation devices, even without protocol optimization.
has issue date	2011-05-21(xsd:dateTime)
bibo:doi	10.1007/s10544-011-9552-4
bibo:pmid	21603962
has license	no-cc
sha1sum (hex)	86cfa075ea4be30b5650b86fa7e15f540fd30be7
schema:url	https://doi.org/10.1007/s10544-011-9552-4
resource representing a document's title	On-chip analysis of respiratory viruses from nasopharyngeal samples
has PubMed Central identifier	PMC7087868
has PubMed identifier	21603962
schema:publication	Biomed Microdevices
resource representing a document's body	covid:86cfa075ea4be30b5650b86fa7e15f540fd30be7#body_text
is schema:about of	named entity 'personalized' covid:arg/86cfa075ea4be30b5650b86fa7e15f540fd30be7 named entity 'infectious diseases' named entity 'Point-of-care' named entity 'PoC' named entity 'PCR' named entity 'Reverse transcription' named entity 'PCR' named entity 'stepper motor' named entity 'nucleic acid' named entity 'on-chip' named entity 'heat transfer' named entity 'lysis' named entity 'on-chip' named entity 'solid phase extraction' named entity 'lab-on-a-chip' named entity 'cross-contamination' named entity 'nucleic acids' named entity 'PCR' named entity 'lysis' named entity 'Fumu' named entity 'Virus' named entity 'biotin' named entity 'R-phycoerythrin' named entity 'sensitivities and specificities' named entity 'microfluidic' named entity 'phycoerythrin' named entity 'syringe' named entity 'in-vitro' named entity 'BioRobot' named entity 'lysis' named entity 'forced convection' named entity 'INFA' named entity 'heating rate' named entity 'viruses' named entity 'PCR' named entity 'PCR' named entity 'RT-PCR' named entity 'commercial end' named entity 'lysis' named entity 'lysis' named entity 'reverse transcription' named entity 'SPE' named entity 'reagent' named entity 'SBS' named entity 'PCR' named entity 'permanent magnet' named entity 'homogenization' named entity 'RT-PCR' named entity 'PCR' named entity 'pathogens' named entity 'DNA sequences' named entity 'lysis' named entity 'freeze-dried' named entity 'reverse transcription PCR' named entity 'bacteriophage' named entity 'fluorescence' named entity 'reagent' named entity 'PCR' named entity 'gDNA' named entity 'polypropylene' named entity 'chromosomal' named entity 'streptavidin' named entity 'PCR' named entity 'PCR' named entity 'PCR'

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