About: Molecular characterization of a new PToV strain. Evolutionary implications

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Molecular characterization of a new PToV strain. Evolutionary implications
Creator	Lavazza, A Pignatelli, J Jimenez, M Luque, J Rejas, M Rodriguez, D
Source	Elsevier; Medline; PMC
abstract	Abstract Toroviruses are emergent viruses, belonging to the Nidovirales order, that remain mostly ignored, despite they are able to infect different species of domestic animals and humans, causing enteric diseases and diarrhea. Thus far, only five variants of porcine torovirus (PToV) have been identified. In this report we describe the identification and partial characterization of a new strain of porcine torovirus (PToV-BRES) that was detected by RT-PCR in a swine faecal specimen from a farm in Brescia (Italy). The complete genes coding for the nucleocapsid (N), hemagglutinin-esterase (HE) and membrane (M) proteins were amplified, and sequence analysis showed that PToV-BRES is a new PToV strain that, based on the HE gene sequence, is phylogenetically related to P4 strain, that was up to now the only member of a distinct PToV lineage. The nucleocapsid protein from PToV-BRES was expressed in insect cells as a his-tagged protein, purified by affinity chromatography and used to develop an ELISA method to detect antibodies against PToV. This assay was evaluated using a serum collection including 45 samples from three commercial farms from Spain. High antibody prevalence against PToV was observed in the three farms, both in adult animals and in piglets, which could suggest that PToV might be endemic in Spanish porcine population. The ELISA method developed in this work could be useful in future epidemiological surveys about toroviruses.
has issue date	2009-07-31(xsd:dateTime)
bibo:doi	10.1016/j.virusres.2009.02.019
bibo:pmid	19463719
has license	els-covid
sha1sum (hex)	86d626bc8e5985b1114bc4b6c21b4b8f1b45a64a
schema:url	https://doi.org/10.1016/j.virusres.2009.02.019
resource representing a document's title	Molecular characterization of a new PToV strain. Evolutionary implications
has PubMed Central identifier	PMC7114482
has PubMed identifier	19463719
schema:publication	Virus Research
resource representing a document's body	covid:86d626bc8e5985b1114bc4b6c21b4b8f1b45a64a#body_text
is schema:about of	named entity 'CHARACTERIZATION' named entity 'torovirus' named entity 'genes' named entity 'domestic animals' named entity 'belonging' named entity 'enteric' named entity 'strain' named entity 'implications' named entity 'USEFUL' named entity 'PURIFIED' named entity 'FAECAL' named entity 'SWINE' named entity 'AFFINITY CHROMATOGRAPHY' named entity 'PROTEIN ' named entity 'ANTIBODY' named entity 'OBSERVED' named entity 'GENES' named entity 'HAVE' named entity 'HIS-' named entity 'ELISA' named entity 'SPANISH' named entity 'RT-PCR' named entity 'PARTIAL' named entity 'ORDER' named entity 'SEQUENCE ANALYSIS' named entity 'RELATED' named entity 'USED' named entity 'SURVEYS' named entity 'REPORT' named entity 'ESTERASE' named entity 'BELONGING' named entity 'MIGHT BE' named entity 'INCLUDING' named entity 'DETECTED' named entity 'CHARACTERIZATION' named entity 'DIARRHEA' named entity 'ASSAY' named entity 'CELLS' named entity 'SAMPLES' named entity 'DISEASES' named entity 'ENDEMIC' named entity 'SEQUENCE' named entity 'PORCINE' named entity 'ANIMALS' named entity 'UP TO' named entity 'LINEAGE' named entity 'SUGGEST' named entity 'ADULT' named entity 'IDENTIFICATION' named entity 'TOROVIRUSES' named entity 'BASED' named entity 'CAUSING' named entity 'METHOD' named entity 'ABOUT' named entity 'EPIDEMIOLOGICAL' named entity 'HIGH' named entity 'NIDOVIRALES' named entity 'VIRUSES' named entity 'HUMANS' named entity 'DOMESTIC ANIMALS' named entity 'EMERGENT' named entity 'STRAIN' named entity 'GENE' named entity 'DIFFERENT' named entity 'SERUM' named entity 'PORCINE TOROVIRUS' named entity 'COMMERCIAL' named entity 'FARMS' named entity 'FAR' named entity 'CODING' named entity 'ENTERIC' named entity 'SPECIMEN' named entity 'WORK' named entity 'IS A' named entity 'EVALUATED' named entity 'MOLECULAR'

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