About: Biochemical characterization of a recombinant SARS coronavirus nsp12 RNA-dependent RNA polymerase capable of copying viral RNA templates

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About: Biochemical characterization of a recombinant SARS coronavirus nsp12 RNA-dependent RNA polymerase capable of copying viral RNA templates Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Biochemical characterization of a recombinant SARS coronavirus nsp12 RNA-dependent RNA polymerase capable of copying viral RNA templates
Creator	Oh, Jong-Won Taylor, Deborah Ahn, Dae-Gyun Choi, Jin-Kyu
Source	Medline; PMC
abstract	The severe acute respiratory syndrome coronavirus (SARS-CoV) RNA genome is replicated by a virus-encoded RNA replicase, the key component of which is the nonstructural protein 12 (nsp12). In this report, we describe the biochemical properties of a full-length recombinant SARS-CoV nsp12 RNA-dependent RNA polymerase (RdRp) capable of copying viral RNA templates. The purified SARS-CoV nsp12 showed both primer-dependent and primer-independent RNA synthesis activities using homopolymeric RNA templates. The RdRp activity was strictly dependent on Mn(2+). The nsp12 preferentially copied homopolymeric pyrimidine RNA templates in the absence of an added oligonucleotide primer. It was also able to initiate de novo RNA synthesis from the 3’-ends of both the plus- and minus-strand genome of SARS-CoV, using the 3’-terminal 36- and 37-nt RNA, respectively. The in vitro RdRp assay system established with a full-length nsp12 will be useful for understanding the mechanisms of coronavirus replication and for the development of anti-SARS-CoV agents.
has issue date	2012-07-13(xsd:dateTime)
bibo:doi	10.1007/s00705-012-1404-x
bibo:pmid	22791111
has license	no-cc
sha1sum (hex)	876ec0cf1a1ee5f9dbb8fe1c0269a2e51c071e38
schema:url	https://doi.org/10.1007/s00705-012-1404-x
resource representing a document's title	Biochemical characterization of a recombinant SARS coronavirus nsp12 RNA-dependent RNA polymerase capable of copying viral RNA templates
has PubMed Central identifier	PMC7086750
has PubMed identifier	22791111
schema:publication	Arch Virol
resource representing a document's body	covid:876ec0cf1a1ee5f9dbb8fe1c0269a2e51c071e38#body_text
is schema:about of	named entity 'RdRp' named entity 'coronavirus' named entity 'RNA' covid:arg/876ec0cf1a1ee5f9dbb8fe1c0269a2e51c071e38 named entity 'genome' named entity 'RNA replicase' named entity 'de novo' named entity 'assay' named entity 'viral' named entity 'homopolymeric' named entity 'activity' named entity 'RdRp' named entity 'pyrimidine' named entity 'minus-strand' named entity 'RNA-dependent RNA polymerase' named entity 'primer' named entity 'SARS-CoV' named entity 'recombinant' named entity 'RNA-dependent RNA polymerase' named entity 'SARS coronavirus' named entity 'nsp4' named entity 'RT-PCR' named entity 'recombinant' named entity '3'-UTR' named entity 'Pfu polymerase' named entity 'poly(A) tail' named entity 'BamHI' named entity 'West Nile' named entity 'poly(A) tail' named entity 'histidine' named entity 'minus-strand' named entity 'genome' named entity 'RdRp' named entity 'ion' named entity 'RdRp' named entity 'RdRp' named entity 'tryptic' named entity 'recombinant' named entity 'subgenomic RNAs' named entity 'bacterial DNA' named entity 'complementary strand' named entity 'GST' named entity 'RNA synthesis' named entity 'SARS-CoV' named entity 'RNA' named entity 'viral genome' named entity 'SARS-CoV' named entity 'viral RNA' named entity '3'-UTR' named entity 'replicase' named entity 'RdRp' named entity 'wild-type' named entity 'N-terminal' named entity 'genome' named entity 'SARS-CoV' named entity 'RdRp' named entity '3'-UTR' named entity 'poly(A) tail' named entity 'poly(A) tail' named entity 'RNA synthesis' named entity 'exogenous' named entity 'homopolymeric' named entity 'RNA' named entity 'pseudoknot' named entity 'viral genome' named entity 'amino acid sequence' named entity 'kDa' named entity 'MS/MS' named entity 'ribonucleotides' named entity 'urea' named entity 'polymerase' named entity 'pH 8' named entity 'ribo' named entity 'RdRp' named entity 'SARS-CoV' named entity 'bona fide' named entity 'RNA' named entity '3' ends'

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