About: Human antibody reaction against recombinant salivary proteins of Phlebotomus orientalis in Eastern Africa

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About: Human antibody reaction against recombinant salivary proteins of Phlebotomus orientalis in Eastern Africa Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Human antibody reaction against recombinant salivary proteins of Phlebotomus orientalis in Eastern Africa
Creator	Hailu, Asrat Oliveira, Fabiano Spitzova, Tatiana Volf, Petr Warburg, Alon Elnaiem, Dia-Eldin Guimaraes-Costa, Anderson Osman, Maha Valenzuela, Jesus Id, Michal Sumova Id, Petra
source	PMC
abstract	BACKGROUND: Phlebotomus orientalis is a vector of Leishmania donovani, the causative agent of life threatening visceral leishmaniasis spread in Eastern Africa. During blood-feeding, sand fly females salivate into the skin of the host. Sand fly saliva contains a large variety of proteins, some of which elicit specific antibody responses in the bitten hosts. To evaluate the exposure to sand fly bites in human populations from disease endemic areas, we tested the antibody reactions of volunteers' sera against recombinant P. orientalis salivary antigens. METHODOLOGY/PRINCIPAL FINDINGS: Recombinant proteins derived from sequence data on P. orientalis secreted salivary proteins, were produced using either bacterial (five proteins) or mammalian (four proteins) expression systems and tested as antigens applicable for detection of anti-P. orientalis IgG in human sera. Using these recombinant proteins, human sera from Sudan and Ethiopia, countries endemic for visceral leishmaniasis, were screened by ELISA and immunoblotting to identify the potential markers of exposure to P. orientalis bites. Two recombinant proteins; mAG5 and mYEL1, were identified as the most promising antigens showing high correlation coefficients as well as good specificity in comparison to the whole sand fly salivary gland homogenate. Combination of both proteins led to a further increase of correlation coefficients as well as both positive and negative predictive values of P. orientalis exposure. CONCLUSIONS/SIGNIFICANCE: This is the first report of screening human sera for anti-P. orientalis antibodies using recombinant salivary proteins. The recombinant salivary proteins mYEL1 and mAG5 proved to be valid antigens for screening human sera from both Sudan and Ethiopia for exposure to P. orientalis bites. The utilization of equal amounts of these two proteins significantly increased the capability to detect anti-P. orientalis antibody responses.
has issue date	2018-12-04(xsd:dateTime)
bibo:doi	10.1371/journal.pntd.0006981
bibo:pmid	30513081
has license	cc0
sha1sum (hex)	87cce3d733ad6f5f0d283d58b8a2b1a0dc47f99e
schema:url	https://doi.org/10.1371/journal.pntd.0006981
resource representing a document's title	Human antibody reaction against recombinant salivary proteins of Phlebotomus orientalis in Eastern Africa
has PubMed Central identifier	PMC6279015
has PubMed identifier	30513081
schema:publication	PLoS Negl Trop Dis
resource representing a document's body	covid:87cce3d733ad6f5f0d283d58b8a2b1a0dc47f99e#body_text
is schema:about of	named entity 'HUMAN' named entity 'AREAS' named entity 'SALIVARY' named entity 'VECTOR' named entity 'DISEASE' named entity 'VARIETY' named entity 'LIFE THREATENING' named entity 'life' named entity 'reaction' named entity 'ANTIBODY' named entity 'LEISHMANIA DONOVANI' named entity 'SERA' named entity 'RECOMBINANT' named entity 'FLY BITES' named entity 'FEEDING' named entity 'REACTIONS' named entity 'SPREAD' named entity 'BITTEN' named entity 'SALIVATE' named entity 'PROTEINS' named entity 'VISCERAL LEISHMANIASIS' named entity 'SALIVA' named entity 'EASTERN AFRICA' named entity 'HUMAN' named entity 'RECOMBINANT' named entity 'SALIVARY PROTEINS' named entity 'EASTERN AFRICA' named entity 'REACTION' named entity 'LARGE' named entity 'FEMALES' named entity 'ENDEMIC' named entity 'BLOOD' named entity 'IS A' named entity 'TESTED' named entity 'ANTIBODY' named entity 'SKIN' named entity 'ELICIT' named entity 'SPECIFIC ANTIBODY' named entity 'VOLUNTEERS' named entity 'RESPONSES' named entity 'CAUSATIVE AGENT' named entity 'PHLEBOTOMUS ORIENTALIS' named entity 'PHLEBOTOMUS ORIENTALIS' named entity 'HOST' named entity 'EVALUATE' named entity 'SAND FLY' named entity 'ANTIGENS' named entity 'POPULATIONS' named entity 'CONTAINS' covid:arg/87cce3d733ad6f5f0d283d58b8a2b1a0dc47f99e named entity 'antibody' named entity 'human populations' named entity 'host' named entity 'Eastern Africa' named entity 'endemic' named entity 'recombinant' named entity 'saliva' named entity 'antibody' named entity 'antibody' named entity 'endemic areas' named entity 'antigens' named entity 'visceral leishmaniasis' named entity 'Leishmania donovani' named entity 'antibody reaction' named entity 'specific' named entity 'sand fly' named entity 'Eastern Africa' named entity 'Phlebotomus' named entity 'sand fly' named entity 'recombinant' named entity 'causative agent'

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