About: Presence of endogenous viral elements negatively correlates with FeLV susceptibility in puma and domestic cat cells

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About: Presence of endogenous viral elements negatively correlates with FeLV susceptibility in puma and domestic cat cells Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Presence of endogenous viral elements negatively correlates with FeLV susceptibility in puma and domestic cat cells
Creator	Chiu, Elliott
Source	BioRxiv
abstract	While feline leukemia virus (FeLV) has been shown to infect felid species other than the endemic domestic cat host, differences in FeLV susceptibility among species has not been evaluated. Previous reports have noted a negative correlation between enFeLV copy number and exogenous FeLV infection outcomes in domestic cats. Since felids outside the genus Felis do not harbor enFeLV genomes, we hypothesized absence of enFeLV results in more severe disease consequences in felid species lacking these genomic elements. We infected primary fibroblasts isolated from domestic cats (Felis catus) and pumas (Puma concolor) with FeLV and quantitated proviral and viral antigen loads. Domestic cat enFeLV env and LTR copy numbers were determined for each individual and compared to FeLV viral outcomes. FeLV proviral and antigen levels were also measured in 6 naturally infected domestic cats and 11 naturally infected Florida panthers (P. concolor coryi). We demonstrated that puma fibroblasts are more permissive to FeLV than domestic cat cells, and domestic cat FeLV restriction was highly related to enFeLV LTR copy number. Terminal tissues from FeLV-infected Florida panthers and domestic cats had similar exFeLV proviral copy numbers, but Florida panther tissues have higher FeLV antigen loads. Our work indicates enFeLV LTR elements negatively regulate exogenous FeLV replication. Further, Puma concolor lacking enFeLV are more permissive to FeLV infection than domestic cats, suggesting endogenization can play a beneficial role in mitigating exogenous retroviral infections. Conversely, presence of endogenous retroelements may relate to new host susceptibility during viral spillover events. Importance Feline leukemia virus (FeLV) can infect a variety of felid species. Only the primary domestic cat host and related small cat species harbor a related endogenous virus in their genomes. Previous studies noted a negative association between the endogenous virus copy number and exogenous virus infection in domestic cats. This report shows that puma cells, which lack endogenous FeLV, produce more virus more rapidly than domestic cat fibroblasts following cell culture challenge. We document a strong association between domestic cat cell susceptibility and FeLV long terminal repeat (LTR) copy number, similar to observations in natural FeLV infections. Viral replication does not, however, correlate with FeLV env copy number, suggesting this effect is specific to FeLV LTR elements. This discovery indicates a protective capacity of the endogenous virus against the exogenous form, either via direct interference or indirectly via gene regulation, and may suggest evolutionary outcomes of retroviral endogenization.
has issue date	2020-06-24(xsd:dateTime)
bibo:doi	10.1101/2020.06.23.168351
has license	biorxiv
sha1sum (hex)	880252a95c062be7df3b64e08fb79d20084a2eee
schema:url	https://doi.org/10.1101/2020.06.23.168351
resource representing a document's title	Presence of endogenous viral elements negatively correlates with FeLV susceptibility in puma and domestic cat cells
schema:publication	bioRxiv
resource representing a document's body	covid:880252a95c062be7df3b64e08fb79d20084a2eee#body_text
is schema:about of	named entity 'cells' named entity 'Title' named entity 'antigen' named entity 'FeLV' named entity 'antigen' named entity 'fibroblasts' named entity 'word count' named entity 'FeLV' named entity 'supernatant' named entity 'puma' named entity 'DMEM' named entity 'fibroblast' named entity 'proviral' named entity 'RNAs' named entity 'endogenous virus' named entity 'LTRs' named entity 'Endogenous' named entity 'FeLV' named entity 'virus strain' named entity 'genus' named entity 'regression analysis' named entity 'retroviral' named entity 'copy numbers' named entity 'fibroblasts' named entity 'tissue cultures' named entity 'antigen' named entity 'puma' named entity 'retroelements' named entity 'genetic elements' named entity 'Florida' named entity 'puma' named entity 'FeLV' named entity 'reservoir host' named entity 'host cell' named entity 'domestic cat' named entity 'epizootics' named entity 'biological processes' named entity 'felid' named entity 'endogenous virus' named entity 'FeLV' named entity 'copy number' named entity 'domestic cat' named entity 'endogenous' named entity 'epidemic' named entity 'cell culture' named entity 'CCR5' named entity 'retroviral' named entity 'ERV' named entity 'puma' named entity 'immunodeficiency' named entity 'NaCl' named entity '2.0' named entity 'multiplicity of infection' named entity 'lymphoid tissues' named entity 'diluent' named entity 'proviral' named entity 'domestic cats' named entity 'FeLV' named entity 'capsid' named entity 'puma' named entity 'exogenous' named entity 'serum' named entity 'virus' named entity 'transcription' named entity 'infection' named entity 'infection status' named entity 'antigen' named entity 'contact inhibition' named entity 'small interfering RNAs' named entity 'domestic cat' named entity 'negatively correlated' named entity 'penicillin' named entity 'endogenous FeLV' named entity 'EDTA' named entity 'infectivity' named entity 'domestic cat' named entity 'wild felids' named entity 'Nalgene' named entity 'virions' named entity 'env' named entity 'copy number variation' named entity 'domestic cats' named entity 'antigen' named entity 'domestic cat'

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