About: Identification of MicroRNA-Like RNAs in Mycelial and Yeast Phases of the Thermal Dimorphic Fungus Penicillium marneffei

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About: Identification of MicroRNA-Like RNAs in Mycelial and Yeast Phases of the Thermal Dimorphic Fungus Penicillium marneffei Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Identification of MicroRNA-Like RNAs in Mycelial and Yeast Phases of the Thermal Dimorphic Fungus Penicillium marneffei
Creator	Yuen, Kwok-Yung Zhang, Na Chow, Wang-Ngai Bao, Jessie Lok, Si Lau, Susanna Wong, Annette Woo, Patrick Yeung, Julian
topic	covid:881abbe78ba9d6d7a690c15464342d1730f327cd#this
Source	PMC
abstract	BACKGROUND: Penicillium marneffei is the most important thermal dimorphic fungus causing systemic mycosis in China and Southeast Asia. While miRNAs are increasingly recognized for their roles in post-transcriptional regulation of gene expression in animals and plants, miRNAs in fungi were less well studied and their potential roles in fungal dimorphism were largely unknown. Based on P. marneffei genome sequence, we hypothesize that miRNA-like RNAs (milRNAs) may be expressed in the dimorphic fungus. METHODOLOGY/PRINCIPAL FINDINGS: We attempted to identify milRNAs in P. marneffei in both mycelial and yeast phase using high-throughput sequencing technology. Small RNAs were more abundantly expressed in mycelial than yeast phase. Sequence analysis revealed 24 potential milRNA candidates, including 17 candidates in mycelial and seven in yeast phase. Two genes, dcl-1 and dcl-2, encoding putative Dicer-like proteins and the gene, qde-2, encoding Argonaute-like protein, were identified in P. marneffei. Phylogenetic analysis showed that dcl-2 of P. marneffei was more closely related to the homologues in other thermal dimorphic pathogenic fungi than to Penicillium chrysogenum and Aspergillus spp., suggesting the co-evolution of dcl-2 among the thermal dimorphic fungi. Moreover, dcl-2 demonstrated higher mRNA expression levels in mycelial than yeast phase by 7 folds (P<0.001). Northern blot analysis confirmed the expression of two milRNAs, PM-milR-M1 and PM-milR-M2, only in mycelial phase. Using dcl-1(KO), dcl-2(KO), dcl(DKO) and qde-2(KO) deletion mutants, we showed that the biogenesis of both milRNAs were dependent on dcl-2 but not dcl-1 or qde-2. The mRNA expression levels of three predicted targets of PM-milR-M1 were upregulated in knockdown strain PM-milR-M1 (KD), supporting regulatory function of milRNAs. CONCLUSIONS/SIGNIFICANCE: Our findings provided the first evidence for differential expression of milRNAs in different growth phases of thermal dimorphic fungi and shed light on the evolution of fungal proteins involved in milRNA biogenesis and possible role of post-transcriptional control in governing thermal dimorphism.
has issue date	2013-08-22(xsd:dateTime)
bibo:doi	10.1371/journal.pntd.0002398
bibo:pmid	23991243
has license	cc-by
sha1sum (hex)	881abbe78ba9d6d7a690c15464342d1730f327cd
schema:url	https://doi.org/10.1371/journal.pntd.0002398
resource representing a document's title	Identification of MicroRNA-Like RNAs in Mycelial and Yeast Phases of the Thermal Dimorphic Fungus Penicillium marneffei
has PubMed Central identifier	PMC3749987
has PubMed identifier	23991243
schema:publication	PLoS Negl Trop Dis
resource representing a document's body	covid:881abbe78ba9d6d7a690c15464342d1730f327cd#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/881abbe78ba9d6d7a690c15464342d1730f327cd
is schema:about of	named entity 'MICRORNA' named entity 'THERMAL' named entity 'PLANTS' named entity 'POST-TRANSCRIPTIONAL REGULATION' named entity 'DIMORPHIC FUNGUS' named entity 'UNKNOWN' named entity 'DIMORPHISM' named entity 'BASED' named entity 'POTENTIAL' named entity 'SYSTEMIC MYCOSIS' named entity 'GENOME SEQUENCE' named entity 'CAUSING' named entity 'LIKE' named entity 'FUNGI ' named entity 'ANIMALS' named entity 'PENICILLIUM MARNEFFEI' named entity 'LARGELY' named entity 'MAY BE' named entity 'YEAST' named entity 'MYCELIAL' named entity 'PHASES' named entity 'LIKE' named entity 'DIMORPHIC FUNGUS' named entity 'RNAS' named entity 'PENICILLIUM MARNEFFEI' named entity 'BACKGROUND' named entity 'THEIR' named entity 'THERMAL' named entity 'CHINA' named entity 'REGULATION OF GENE EXPRESSION' named entity 'RNAS' named entity 'MIRNAS' named entity 'IDENTIFICATION' named entity 'IMPORTANT' named entity 'MIRNA' named entity 'ROLES' named entity 'EXPRESSED' named entity 'SOUTHEAST ASIA' covid:arg/881abbe78ba9d6d7a690c15464342d1730f327cd named entity 'genome sequence' named entity 'RNAs' named entity 'Yeast' named entity 'Mycelial' named entity 'Penicillium marneffei' named entity 'Dimorphic Fungus' named entity 'transposable elements' named entity 'sequencing reads' named entity 'bioinformatics' named entity 'genome' named entity 'genomes' named entity 'miRNAs' named entity 'Northern blot analyses' named entity 'small RNA' named entity 'benzoate 4-monooxygenase' named entity 'mycelial' named entity 'homologues' named entity 'Southeast Asia' named entity 'gene sequences' named entity 'dimorphic fungus' named entity 'RNAi silencing' named entity 'yeasts' named entity 'dimorphic fungi' named entity 'fungi' named entity 'fungus' named entity 'Sigma-Aldrich' named entity 'Aspergillus' named entity 'gene' named entity 'secondary metabolism' named entity 'gene' named entity 'protein sequences' named entity 'internal transcribed spacer'

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