About: Mixed infections in vitro with different Chlamydiaceae strains and a cell culture adapted porcine epidemic diarrhea virus

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About: Mixed infections in vitro with different Chlamydiaceae strains and a cell culture adapted porcine epidemic diarrhea virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Mixed infections in vitro with different Chlamydiaceae strains and a cell culture adapted porcine epidemic diarrhea virus
Creator	Grest, Paula Pospischil, Andreas Schiller, Irene Stuedli, Angela
Source	Elsevier; Medline; PMC
abstract	Abstract Assuming a synergistic or additive effect of Chlamydiaceae in coexistence with other enteropathogenic agents, the viral/bacterial interaction between a cell culture adapted porcine epidemic diarrhea virus (ca-PEDV) and different Chlamydiaceae strains was studied in vitro. Vero cells were dually infected with ca-PEDV and one of the three chlamydial strains Chlamydia trachomatis S45, Chlamydophila abortus S26/3 or Chlamydophila pecorum 1710S. Three experimental protocols were designed varying the inoculation sequence. Cell layers were first inoculated with Chlamydiaceae and 20h later with ca-PEDV in protocol one. In protocol two, both agents were administered concurrently, whereas in protocol three, ca-PEDV was applied 20h in advance of the Chlamydiaceae. Immunofluorescence techniques, immunohistochemical (IH) staining and electron microscopy were subsequently employed to investigate the cell layers. Using indirect immunofluorescence (IF) labeling, all mixed infections revealed dually infected cells, however, only incidentally and in low numbers. Characteristically, ca-PEDV syncytia with one or more chlamydial inclusions were detected but dually infected single cells were absent. Some syncytial cells contained enlarged C. abortus or C. pecorum inclusions with abnormally large developmental forms. In comparison with simultaneously conducted monoinfections, larger chlamydial inclusions were observed in dually infected cell layers. Experiments with C. trachomatis showed significantly increased numbers of chlamydial inclusions in dually infected cell layers compared to monoinfected ones. These findings indicate an influence of ca-PEDV on the chlamydial developmental cycle and in the case of C. trachomatis, a positive effect on chlamydial colonization in mixed infections.
has issue date	2005-04-10(xsd:dateTime)
bibo:doi	10.1016/j.vetmic.2004.10.023
bibo:pmid	15778027
has license	els-covid
sha1sum (hex)	884fa3917f4eb5915950c7462f1fc5158cb5d06f
schema:url	https://doi.org/10.1016/j.vetmic.2004.10.023
resource representing a document's title	Mixed infections in vitro with different Chlamydiaceae strains and a cell culture adapted porcine epidemic diarrhea virus
has PubMed Central identifier	PMC7126122
has PubMed identifier	15778027
schema:publication	Veterinary Microbiology
resource representing a document's body	covid:884fa3917f4eb5915950c7462f1fc5158cb5d06f#body_text
is schema:about of	named entity 'CHLAMYDIACEAE' named entity 'INFECTIONS' named entity 'adapted' named entity 'coexistence' named entity 'protocols' named entity 'infected' named entity 'cycle' named entity 'case' named entity 'immunohistochemical' named entity 'S45' named entity 'sequence' covid:arg/884fa3917f4eb5915950c7462f1fc5158cb5d06f named entity 'TO INVESTIGATE' named entity 'STRAINS' named entity 'CELL CULTURE' named entity 'APPLIED' named entity 'THESE' named entity 'S45' named entity 'SEQUENCE' named entity 'INFECTED CELL' named entity 'ELECTRON MICROSCOPY' named entity 'INFECTED' named entity 'SINGLE' named entity 'LATER' named entity 'LARGER' named entity 'CHLAMYDOPHILA PECORUM' named entity 'ADVANCE' named entity 'USING' named entity 'INFECTIONS' named entity 'SYNERGISTIC' named entity 'EXPERIMENTAL' named entity 'ADMINISTERED' named entity 'OBSERVED' named entity 'IN VITRO' named entity 'BACTERIAL' named entity 'LOW' named entity 'CHLAMYDIACEAE' named entity 'SYNCYTIAL' named entity 'VERO CELLS' named entity 'SYNCYTIA' named entity 'VIRAL' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'ADAPTED' named entity 'NUMBERS' named entity 'A CELL' named entity 'CYCLE' named entity 'CASE' named entity 'IMMUNOFLUORESCENCE TECHNIQUES' named entity 'STRAINS' named entity 'EMPLOYED' named entity 'COLONIZATION' named entity 'INOCULATION' named entity 'INFLUENCE' named entity 'ADAPTED' named entity 'IN VITRO' named entity 'A CELL' named entity 'PORCINE EPIDEMIC DIARRHEA VIRUS' named entity 'STAINING' named entity 'ENTEROPATHOGENIC' named entity 'FINDINGS' named entity 'LAYERS' named entity 'PROTOCOLS' named entity 'LABELING' named entity 'MIXED' named entity 'INCREASED' named entity 'ENLARGED' named entity 'MIXED' named entity 'DIFFERENT' named entity 'SIMULTANEOUSLY' named entity 'ONE OF' named entity 'ADDITIVE' named entity 'COMPARISON' named entity 'CHLAMYDOPHILA ABORTUS' named entity 'BUT' named entity 'LARGE' named entity 'REVEALED' named entity 'INDIRECT IMMUNOFLUORESCENCE' named entity 'PROTOCOL'

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