About: Application of ProteinChip Array Profiling in Serum Biomarker Discovery for Patients Suffering From Severe Acute Respiratory Syndrome

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Application of ProteinChip Array Profiling in Serum Biomarker Discovery for Patients Suffering From Severe Acute Respiratory Syndrome Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Application of ProteinChip Array Profiling in Serum Biomarker Discovery for Patients Suffering From Severe Acute Respiratory Syndrome
Creator	Yip, Tai-Tung Cho, William Chan, Johnny Law, Stephen Ngan, Roger Yip, Timothy Cheng, Wai Ma, Victor Yip, Christine
Source	Medline; PMC
abstract	A new strain of coronavirus has caused an outbreak of severe acute respiratory syndrome (SARS) from 2002 to 2003 resulting in 774 deaths worldwide. By protein chip array profiling technology, a number of serum biomarkers that might be useful in monitoring the clinical course of SARS patients were identified. This book chapter describes how the protein chip array profiling was carried out for this study. Briefly, SARS patients’ serum samples were first fractionated in Q Ceramic HyperD ion exchange sorbent beads by buffers at different pH. Serum protein fractions thus obtained were then bound onto a copper (II) immobilized metal affinity capture (IMAC30 Cu [II]) ProteinChip® Array or a weak cation-exchange (CM10) ProteinChip Array. After washing and addition of sinapinic acid, the chips were read in a Protein Biological System (PBS) IIc mass spectrometer. Ions were generated by laser shots and flied in a time of flight mode to the ion detector according to their mass over charge (m/z) ratio. The serum profiling spectra in SARS patients were acquired, baseline subtracted and analyzed in parallel with those from the control subjects by Ciphergen ProteinChip Software 3.0.2 with their peak intensities compared by a nonparametric two sample Mann-Whitney-U test. More than twelve peaks were differentially expressed in SARS patients with one at m/z of 11,695 (later identified to be serum amyloid A protein), which had increase in peak intensity correlating with the extent of SARS-coronavirus induced pneumonia as defined by a serial chest X-ray opacity score. The remaining biomarkers could also be useful in the study of other clinical parameters in SARS patients.
has issue date	2007-01-01(xsd:dateTime)
bibo:doi	10.1007/978-1-59745-304-2_20
bibo:pmid	18220240
has license	no-cc
sha1sum (hex)	88620453204f2890ec9fabae6978a6b271f48e2f
schema:url	https://doi.org/10.1007/978-1-59745-304-2_20
resource representing a document's title	Application of ProteinChip Array Profiling in Serum Biomarker Discovery for Patients Suffering From Severe Acute Respiratory Syndrome
has PubMed Central identifier	PMC7120874
has PubMed identifier	18220240
schema:publication	Microarrays
resource representing a document's body	covid:88620453204f2890ec9fabae6978a6b271f48e2f#body_text
is schema:about of	named entity 'flight mode' named entity 'Ions' named entity 'Array' named entity 'mass' named entity 'desorption' named entity 'profiling' named entity 'worldwide' named entity 'serum' named entity 'study' named entity 'serial' named entity 'compared' named entity 'intensities' named entity 'score' named entity 'Biomarker' covid:arg/88620453204f2890ec9fabae6978a6b271f48e2f named entity 'patients' named entity 'opacity' named entity 'laser' named entity 'identified' named entity 'nonparametric' named entity 'outbreak' named entity 'bound' named entity 'defined' named entity 'profiling' named entity 'fractionated' named entity 'biomarkers' named entity 'protein chip' named entity 'time of flight' named entity 'serum amyloid A' named entity 'Protein' named entity 'mass spectrometer' named entity 'opacity' named entity 'differentially expressed' named entity 'SARS' named entity 'surface-enhanced laser desorption/ionization' named entity 'ion' named entity 'SELDI-TOF' named entity 'protein chip' named entity 'cation-exchange' named entity 'SARS' named entity 'sorbent' named entity 'RT-PCR' named entity 'clinical presentation' named entity 'time of flight' named entity 'elution' named entity 'mass spectrometer' named entity 'Wizard mode' named entity 'collection plate' named entity 'PBS' named entity 'biomarkers' named entity 'May 8th' named entity 'opacity' named entity 'biomarkers' named entity 'flow chart' named entity 'serum' named entity 'serum' named entity 'downregulated' named entity 'adult respiratory distress syndrome' named entity 'laser' named entity 'ultraviolet' named entity 'PBS' named entity 'peptide' named entity 'opacity' named entity 'm/z' named entity 'ionization' named entity 'lung' named entity 'SARS' named entity 'desorbed' named entity 'elute' named entity 'SELDI' named entity 'eluate' named entity 'Incubate' named entity 'SARS'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software