About: Simultaneous detection of eleven sexually transmitted agents using multiplexed PCR coupled with MALDI-TOF analysis

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About: Simultaneous detection of eleven sexually transmitted agents using multiplexed PCR coupled with MALDI-TOF analysis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Simultaneous detection of eleven sexually transmitted agents using multiplexed PCR coupled with MALDI-TOF analysis
Creator	Li, Yamei Peng, Junping Wang, Feng Xiu, Leshan Zhang, Chi
Source	PMC
abstract	PURPOSE: Sexually transmitted infections (STIs), representing a major global health problem, are caused by different microbes, including bacteria, viruses, and protozoa. Unfortunately, infections of different sexually transmitted pathogens often present similar clinical symptoms, so it is almost impossible to distinguish them clinically. Therefore, the aim of the current study was to develop a sensitive, multitarget, and high-throughput method that can detect various agents responsible for STIs. METHODS: We developed and tested a 23-plex PCR coupled with matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) assay (sexually transmitted infection-mass spectrometry, STI-MS) that simultaneously targets 11 different agents, including 8 most common clinical pathogens related to STIs (HSV-1, HSV-2, Neisseria gonorrhoeae, Chlamydia trachomatis, Treponema pallidum, Trichomonas vaginalis, Mycoplasma genitalium, and Haemophilus ducreyi) and 3 controversial microorganisms as pathogens (Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum). RESULTS: The results showed that the STI-MS approach can accurately detect the expected agents, without cross-reaction with other organisms. The limit of detection of each STI-MS assay was ranged from 1.739 to 10.009 copies/reaction, using probit analyses. The verification rate for each target organism of the STI-MS ranged from a minimum of 89.3% to a maximum of 100%, using conventional assays and ultrasensitive digital PCR to confirm the STI-MS-positive results. To further evaluate the clinical performance of this assay, 241 clinical specimens (124 urethral/cervical swabs and 117 urine) were tested in parallel using the STI-MS assay and monoplex real-time PCR for each agent. The overall validation parameters of STI-MS were extremely high including sensitivity (from 85.7% to 100%), specificity (from 92.3% to 100%), PPV (from 50% to 100%), and NPV (from 99.1% to 100%) for each target. CONCLUSION: STI-MS is a useful high-throughput screening tool for detecting mixed infections of STIs and has great potential for application in large-scale epidemiological programs for specific microorganisms of STI.
has issue date	2019-08-28(xsd:dateTime)
bibo:doi	10.2147/idr.s219580
bibo:pmid	31695443
has license	cc-by-nc
sha1sum (hex)	887264fa70bfd2130e5ef9089f058332364d6313
schema:url	https://doi.org/10.2147/idr.s219580
resource representing a document's title	Simultaneous detection of eleven sexually transmitted agents using multiplexed PCR coupled with MALDI-TOF analysis
has PubMed Central identifier	PMC6717854
has PubMed identifier	31695443
schema:publication	Infect Drug Resist
resource representing a document's body	covid:887264fa70bfd2130e5ef9089f058332364d6313#body_text
is schema:about of	named entity 'PRESENT' named entity 'METHOD' named entity 'BACTERIA' named entity 'DETECT' named entity 'THROUGHPUT' named entity 'PROTOZOA' named entity 'CAUSED BY' named entity 'DIFFERENT' named entity 'current' named entity 'PCR' named entity 'PCR' named entity 'CLINICAL SYMPTOMS' named entity 'HIGH' named entity 'INFECTIONS' named entity 'MICROBES' covid:arg/887264fa70bfd2130e5ef9089f058332364d6313 named entity 'ALMOST' named entity 'VARIOUS' named entity 'SEXUALLY TRANSMITTED INFECTIONS' named entity 'INCLUDING' named entity 'USING' named entity 'SEXUALLY TRANSMITTED AGENTS' named entity 'ANALYSIS' named entity 'COUPLED' named entity 'MALDI-TOF' named entity 'DETECTION' named entity 'SIMULTANEOUS' named entity 'SEXUALLY TRANSMITTED' named entity 'VIRUSES' named entity 'IMPOSSIBLE' named entity 'A MAJOR' named entity 'SENSITIVE' named entity 'STUDY' named entity 'PATHOGENS' named entity 'SIMILAR' named entity 'RESPONSIBLE' named entity 'HEALTH PROBLEM' named entity 'CURRENT' named entity 'AIM' named entity 'GLOBAL HEALTH' named entity 'PURPOSE' named entity 'sexually transmitted' named entity 'STIs' named entity 'STIs' named entity 'sensitivity, specificity' named entity 'public health interventions' named entity 'T. pallidum' named entity 'antigen' named entity 'serology' named entity 'commensalism' named entity 'M. hominis' named entity 'false-negative' named entity 'microorganisms' named entity 'World Health Organization' named entity 'primers' named entity 'Probit analysis' named entity 'microorganisms' named entity 'Treponema pallidum' named entity 'mass spectrometer' named entity 'Digital PCR' named entity 'Institutional Review Boards' named entity 'SBE' named entity 'Neisseria gonorrhoeae' named entity 'real-time PCR' named entity 'high-throughput method' named entity 'nested PCR' named entity 'digital PCR' named entity 'bacteria' named entity 'DNA samples' named entity 'PCR' named entity 'reagent' named entity 'STIs' named entity 'pathogens' named entity 'homology' named entity 'antigen' named entity 'pathogen' named entity 'H. ducreyi'

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