About: The Capsid Protein VP1 of Coxsackievirus B Induces Cell Cycle Arrest by Up-Regulating Heat Shock Protein 70

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About: The Capsid Protein VP1 of Coxsackievirus B Induces Cell Cycle Arrest by Up-Regulating Heat Shock Protein 70 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The Capsid Protein VP1 of Coxsackievirus B Induces Cell Cycle Arrest by Up-Regulating Heat Shock Protein 70
Creator	Wang, Yan Zhang, Jian Chen, Yang Zhong, Zhaohua Wang, Y Dong, Chaorun Dong, Yanyan Feng, Xiaofeng Qu, Cong Wang, Tianying Wang, Yao Wo, Xiaoman Xu, Weizhen Zhao, Shuoxuan Zhao, Wenran Chen, Ruizhen Li, Minghui Kainov, Denis Hanson, Paul
Source	PMC
abstract	Manipulating cell cycle is one of the common strategies used by viruses to generate favorable cellular environment to facilitate viral replication. Coxsackievirus B (CVB) is one of the major viral pathogens of human myocarditis and cardiomyopathy. Because of its small genome, CVB depends on cellular machineries for productive replication. However, how the structural and non-structural components of CVB would manipulate cell cycle is not clearly understood. In this study, we demonstrated that the capsid protein VP1 of CVB type 3 (CVB3) induced cell cycle arrest at G1 phase. G1 arrest was the result of the decrease level of cyclin E and the accumulation of p27(Kip1). Study on the gene expression profile of the cells expressing VP1 showed that the expression of both heat shock protein 70-1 (Hsp70-1) and Hsp70-2 was significantly up-regulated. Knockdown of Hsp70 resulted in the increased level of cyclin E and the reduction of p27(Kip1). We further demonstrated that the phosphorylation of the heat shock factor 1, which directly promotes the expression of Hsp70, was also increased in the cell expressing VP1. Moreover, we show that CVB3 infection also induced G1 arrest, likely due to dysregulating Hsp70, cyclin E, and p27, while knockdown of Hsp70 dramatically inhibited viral replication. Cell cycle arrest at G1 phase facilitated CVB3 infection, since viral replication in the cells synchronized at G1 phase dramatically increased. Taken together, this study demonstrates that the VP1 of CVB3 induces cell cycle arrest at G1 phase through up-regulating Hsp70. Our findings suggest that the capsid protein VP1 of CVB is capable of manipulating cellular activities during viral infection.
has issue date	2019-07-17(xsd:dateTime)
bibo:doi	10.3389/fmicb.2019.01633
bibo:pmid	31379784
has license	cc-by
sha1sum (hex)	88c599b1de8a17cd519f9f72aaaea28392f697f0
schema:url	https://doi.org/10.3389/fmicb.2019.01633
resource representing a document's title	The Capsid Protein VP1 of Coxsackievirus B Induces Cell Cycle Arrest by Up-Regulating Heat Shock Protein 70
has PubMed Central identifier	PMC6653663
has PubMed identifier	31379784
schema:publication	Front Microbiol
resource representing a document's body	covid:88c599b1de8a17cd519f9f72aaaea28392f697f0#body_text
is schema:about of	named entity 'viral replication' named entity 'cells' named entity 'Cell cycle' named entity 'arrest' named entity 'expression' named entity 'Hsp70' named entity 'cells' named entity 'gene' named entity 'G1 phase' named entity 'cellular' named entity 'Cell Cycle' named entity 'REGULATING' named entity 'CELL CYCLE ARREST' named entity 'JOURNAL ' named entity 'DIRECTLY' named entity 'PROTEIN ' named entity 'COXSACKIEVIRUS B' named entity 'LEVEL' named entity 'REGULATING' named entity 'REDUCTION' named entity 'G1 PHASE' named entity 'PHOSPHORYLATION' named entity 'GENE EXPRESSION PROFILE' named entity 'INDUCED' named entity 'FINDINGS' named entity 'EXPRESSION' named entity 'COMPONENTS' named entity 'STRUCTURAL' named entity 'CAPSID PROTEIN' named entity 'CITATION' named entity 'TAKEN' named entity 'ONE OF' named entity 'HSP70-2' named entity 'OUR' named entity 'DEMONSTRATED' named entity 'VIRUSES' named entity 'HOW' named entity 'HSP70' named entity 'REPLICATION' named entity 'CVB3' named entity 'CYCLIN E' named entity 'MAJOR' named entity 'CAPABLE' named entity 'SMALL' named entity 'ITS' named entity 'SUGGEST' named entity 'G1 ARREST' named entity 'GENOME' named entity 'CELLULAR' named entity 'DECREASE' named entity 'INCREASED' named entity 'VIRAL REPLICATION' named entity 'P27 KIP1' named entity 'USED BY' named entity 'LIKELY' named entity 'STUDY' named entity 'HUMAN' named entity 'PRODUCTIVE' named entity 'GENERATE' named entity 'CVB' named entity 'UP-REGULATED' named entity 'INFECTION' named entity 'SYNCHRONIZED'

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