About: Analysis of Memory B Cell Responses and Isolation of Novel Monoclonal Antibodies with Neutralizing Breadth from HIV-1-Infected Individuals

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About: Analysis of Memory B Cell Responses and Isolation of Novel Monoclonal Antibodies with Neutralizing Breadth from HIV-1-Infected Individuals Goto Sponge NotDistinct Permalink

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Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Analysis of Memory B Cell Responses and Isolation of Novel Monoclonal Antibodies with Neutralizing Breadth from HIV-1-Infected Individuals
Creator	Corti, Davide Lanzavecchia, Antonio Sallusto, Federica Heeney, Jonathan Weissenhorn, Winfried Jarrossay, David Pinna, Debora Vanzetta, Fabrizia Montefiori, David Silacci, Chiara Weiss, Robin Sattentau, Quentin Davis, David Willems, Betty Fernandez-Rodriguez, Blanca Balla-Jhagjhoorsingh, Sunita Dreja, Hanna Hinz, Andreas Orkin, Chloe Pade, Corinna Jeffs, Simon Langedijk, Johannes Mcknight, Ine O'sullivan, Eithne Seaman, Michael Zekveld, Maria
Source	PMC
abstract	BACKGROUND: The isolation of human monoclonal antibodies (mAbs) that neutralize a broad spectrum of primary HIV-1 isolates and the characterization of the human neutralizing antibody B cell response to HIV-1 infection are important goals that are central to the design of an effective antibody-based vaccine. METHODS AND FINDINGS: We immortalized IgG(+) memory B cells from individuals infected with diverse clades of HIV-1 and selected on the basis of plasma neutralization profiles that were cross-clade and relatively potent. Culture supernatants were screened using various recombinant forms of the envelope glycoproteins (Env) in multiple parallel assays. We isolated 58 mAbs that were mapped to different Env surfaces, most of which showed neutralizing activity. One mAb in particular (HJ16) specific for a novel epitope proximal to the CD4 binding site on gp120 selectively neutralized a multi-clade panel of Tier-2 HIV-1 pseudoviruses, and demonstrated reactivity that was comparable in breadth, but distinct in neutralization specificity, to that of the other CD4 binding site-specific neutralizing mAb b12. A second mAb (HGN194) bound a conserved epitope in the V3 crown and neutralized all Tier-1 and a proportion of Tier-2 pseudoviruses tested, irrespective of clade. A third mAb (HK20) with broad neutralizing activity, particularly as a Fab fragment, recognized a highly conserved epitope in the HR-1 region of gp41, but showed striking assay-dependent selectivity in its activity. CONCLUSIONS: This study reveals that by using appropriate screening methods, a large proportion of memory B cells can be isolated that produce mAbs with HIV-1 neutralizing activity. Three of these mAbs show unusual breadth of neutralization and therefore add to the current panel of HIV-1 neutralizing antibodies with potential for passive protection and template-based vaccine design.
has issue date	2010-01-20(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0008805
bibo:pmid	20098712
has license	cc-by
sha1sum (hex)	88d360849c4d9dddb7c9d16db3e9c4bbb52b7b75
schema:url	https://doi.org/10.1371/journal.pone.0008805
resource representing a document's title	Analysis of Memory B Cell Responses and Isolation of Novel Monoclonal Antibodies with Neutralizing Breadth from HIV-1-Infected Individuals
has PubMed Central identifier	PMC2808385
has PubMed identifier	20098712
schema:publication	PLoS One
resource representing a document's body	covid:88d360849c4d9dddb7c9d16db3e9c4bbb52b7b75#body_text
is schema:about of	named entity 'central' named entity 'HIV-1 infection' named entity 'HIV-1' named entity 'RESPONSES' named entity 'NOVEL' named entity 'BREADTH' named entity 'VACCINE' named entity 'CHARACTERIZATION' named entity 'IMPORTANT' named entity 'HIV-1' named entity 'NEUTRALIZING ANTIBODY' named entity 'ISOLATES' named entity 'ANTIBODY' named entity 'CELL RESPONSE' named entity 'MEMORY B CELL' named entity 'INDIVIDUALS' named entity 'MONOCLONAL ANTIBODIES' named entity 'BACKGROUND' named entity 'HIV-1 INFECTION' named entity 'PRIMARY' named entity 'INFECTED' named entity 'HIV-1' named entity 'ANALYSIS' named entity 'ISOLATION' named entity 'BROAD SPECTRUM' named entity 'DESIGN' named entity 'MONOCLONAL ANTIBODIES' named entity 'RESPONSE TO' named entity 'HUMAN' named entity 'GOALS' named entity 'B CELL' named entity 'EFFECTIVE' named entity 'ISOLATION' named entity 'BASED' named entity 'CENTRAL' covid:arg/88d360849c4d9dddb7c9d16db3e9c4bbb52b7b75 named entity 'response' named entity 'mAbs' named entity 'neutralizing antibody' named entity 'Monoclonal Antibodies' named entity 'NIH' named entity 'neutralizing antibodies' named entity 'HIV-1' named entity 'conformations' named entity 'NIH' named entity 'mAb' named entity 'clade' named entity 'gp41' named entity 'epitope' named entity 'gp41' named entity 'antigens' named entity 'mAb' named entity 'HEK293T' named entity 'transmembrane glycoprotein' named entity 'HIV-1' named entity 'viruses'

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