About: Detection of bovine coronavirus using a TaqMan-based real-time RT-PCR assay

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An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Detection of bovine coronavirus using a TaqMan-based real-time RT-PCR assay
Creator	Buonavoglia, Canio Campolo, Marco Decaro, Nicola Desario, Costantina Elia, Gabriella Mari, Viviana Cirone, Francesco Tempesta, Maria Radogna, Arianna Colaianni, Maria
Source	Elsevier; Medline; PMC
abstract	A real-time reverse transcriptase-polymerase chain reaction (RT-PCR) for the detection of bovine coronavirus (BCoV) RNA in clinical samples is described. The assay is based on TaqMan technology, consisting of two primers and one probe labeled with the reporter dye 6-carboxyfluorescein that binds selectively to the transmembrane-protein gene of BCoV. The BCoV real-time RT-PCR assay was able to detect the tested BCoV and BCoV-like viruses (canine respiratory coronavirus and bubaline coronavirus), whereas other common viral pathogens of cattle were not recognised by the established oligonucleotide set, thus showing that the test was specific for bovine-like CoVs. The detection limit of the assay was 20 BCoV RNA copies (1-log higher with respect to traditional gel-based RT-PCR) and the reproducibility was satisfactory, thus allowing for a sensitive and accurate measurement of the viral RNA load in clinical samples. Two hundred and twenty clinical specimens (92 rectal, 82 nasal and 46 ocular swabs) were subjected to gel-based and real-time RT-PCR. By conventional amplification, 43 rectal, 54 nasal and 34 ocular samples tested positive, whereas the TaqMan assay was able to detect the BCoV nucleic acid in 49 rectal, 60 nasal and 37 ocular swabs. The rapidity and high throughput of the BCoV TaqMan assay makes this method a powerful tool for a sensitive and specific diagnosis of BCoV infection in cattle.
has issue date	2008-06-24(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2008.05.016
bibo:pmid	18579223
has license	no-cc
sha1sum (hex)	88fda71a3c0b92de587a797b3490442822bac919
schema:url	https://doi.org/10.1016/j.jviromet.2008.05.016
resource representing a document's title	Detection of bovine coronavirus using a TaqMan-based real-time RT-PCR assay
has PubMed Central identifier	PMC7112840
has PubMed identifier	18579223
schema:publication	J Virol Methods
resource representing a document's body	covid:88fda71a3c0b92de587a797b3490442822bac919#body_text
is schema:about of	named entity 'COVS' named entity 'METHOD' named entity 'SATISFACTORY' named entity 'TESTED' named entity 'VIRUSES' named entity 'SAMPLES' named entity 'INFECTION' named entity 'REAL-TIME RT-PCR' named entity 'TEST' named entity 'PRIMERS' named entity 'SET' named entity 'RT-PCR' named entity 'REPORTER' named entity 'TWENTY' named entity 'TRADITIONAL' named entity 'COMMON' named entity 'REAL-TIME' named entity 'SPECIMENS' named entity 'PROBE' named entity 'PROTEIN GENE' named entity 'CORONAVIRUS' named entity 'COPIES' named entity 'rectal' named entity 'tested' named entity 'RNA' named entity 'viral' named entity 'positive' named entity 'rectal' named entity 'Two' named entity 'ocular' named entity 'cattle' named entity 'USING' named entity 'BOVINE CORONAVIRUS' named entity 'HIGH THROUGHPUT' named entity 'TRANSMEMBRANE-PROTEIN' named entity 'SENSITIVE' named entity 'RT-PCR ASSAY' named entity 'REAL-TIME RT-PCR' named entity 'BASED' named entity 'TECHNOLOGY' named entity 'ALLOWING' named entity 'RNA' named entity 'POWERFUL' named entity '6-CARBOXYFLUORESCEIN' named entity 'TAQMAN' named entity 'DESCRIBED' named entity 'HIGHER' named entity 'POSITIVE' named entity 'LOAD' named entity 'RESPECT' named entity 'TOOL' named entity 'ESTABLISHED' named entity 'LIKE' named entity 'RT-PCR ASSAY' named entity 'AMPLIFICATION' named entity 'RECTAL' named entity 'SPECIFIC' named entity 'DETECTION' named entity 'VIRAL PATHOGENS' named entity 'MEASUREMENT' named entity 'OCULAR' named entity 'VIRAL RNA' named entity 'GEL' named entity 'OLIGONUCLEOTIDE' named entity 'CLINICAL' named entity 'REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION' named entity 'REPRODUCIBILITY' named entity 'DETECTION LIMIT' named entity 'ASSAY' named entity 'LABELED' named entity 'CONVENTIONAL' named entity 'BASED'

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