About: Analysis of chikungunya virus proteins reveals that non-structural proteins nsP2 and nsP3 exhibit RNA interference (RNAi) suppressor activity

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About: Analysis of chikungunya virus proteins reveals that non-structural proteins nsP2 and nsP3 exhibit RNA interference (RNAi) suppressor activity Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : wasabi.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Analysis of chikungunya virus proteins reveals that non-structural proteins nsP2 and nsP3 exhibit RNA interference (RNAi) suppressor activity
Creator	Anand, Abhishek Mathur, Kalika Sanan-Mishra, Neeti Sunil, Sujatha Bhatnagar, Raj Dubey, Sunil
Source	PMC
abstract	RNAi pathway is an antiviral defence mechanism employed by insects that result in degradation of viral RNA thereby curbing infection. Several viruses including flaviviruses encode viral suppressors of RNAi (VSRs) to counteract the antiviral RNAi pathway. Till date, no VSR has been reported in alphaviruses. The present study was undertaken to evaluate chikungunya virus (CHIKV) proteins for RNAi suppressor activity. We systematically analyzed all nine CHIKV proteins for RNAi suppressor activity using Sf21 RNAi sensor cell line based assay. Two non-structural proteins, namely, nsP2 and nsP3 were found to exhibit RNAi suppressor activity. We further validated the findings in natural hosts, namely in Aedes and in mammalian cell lines and further through EMSA and Agrobacterium infiltration in GFP silenced transgenic tobacco plants. Domains responsible for maximum RNAi suppressor activity were also identified within these proteins. RNA binding motifs in these domains were identified and their participation in RNAi suppression evaluated using site directed mutagenesis. Sequence alignment of these motifs across all species of known alphaviruses revealed conservation of these motifs emphasizing on a similar role of action in other species of alphaviruses as well. Further validation of RNAi suppressor activity of these proteins awaits establishment of specific virus infection models.
has issue date	2016-11-30(xsd:dateTime)
bibo:doi	10.1038/srep38065
bibo:pmid	27901124
has license	cc-by
sha1sum (hex)	89444dffc7f46f0f52709e43550ddb363a720340
schema:url	https://doi.org/10.1038/srep38065
resource representing a document's title	Analysis of chikungunya virus proteins reveals that non-structural proteins nsP2 and nsP3 exhibit RNA interference (RNAi) suppressor activity
has PubMed Central identifier	PMC5128919
has PubMed identifier	27901124
schema:publication	Sci Rep
resource representing a document's body	covid:89444dffc7f46f0f52709e43550ddb363a720340#body_text
is schema:about of	named entity 'CHIKUNGUNYA VIRUS' named entity 'cell' named entity 'RNAi' named entity 'activity' named entity 'action' named entity 'study' named entity 'conservation' named entity 'NSP2' named entity 'REVEALED' named entity 'CHIKUNGUNYA VIRUS' named entity 'ACROSS' named entity 'CONSERVATION' named entity 'ACTION' named entity 'ANALYZED' named entity 'EMSA' named entity 'INFILTRATION' named entity 'SEQUENCE ALIGNMENT' named entity 'ASSAY' named entity 'MAXIMUM' named entity 'ALPHAVIRUSES' named entity 'THESE' named entity 'HOSTS' named entity 'SUPPRESSION' named entity 'ROLE' named entity 'NON-STRUCTURAL PROTEINS' named entity 'PARTICIPATION' named entity 'SF21' named entity 'EVALUATE' named entity 'OPEN' named entity 'REVEALS' named entity 'AGROBACTERIUM' named entity 'IDENTIFIED' named entity 'RESPONSIBLE' named entity 'ESTABLISHMENT' named entity 'PRESENT' named entity 'KNOWN' named entity 'MOTIFS' named entity 'VIRUS INFECTION' named entity 'THEIR' named entity 'SPECIFIC' named entity 'EXHIBIT' named entity 'PROTEINS' named entity 'NSP3' named entity 'EXHIBIT' named entity 'FINDINGS' named entity 'SUPPRESSOR' named entity 'ACTIVITY' named entity 'NON-STRUCTURAL PROTEINS' named entity 'ANALYSIS' named entity 'SILENCED' named entity 'NATURAL' named entity 'SITE DIRECTED MUTAGENESIS' named entity 'USING' named entity 'VALIDATED' named entity 'RNAI' named entity 'TRANSGENIC TOBACCO' named entity 'CELL LINE' named entity 'SUPPRESSOR' named entity 'ACTIVITY' named entity 'SIMILAR' named entity 'PLANTS' named entity 'SENSOR' named entity 'SPECIES' named entity 'VALIDATION' named entity 'STUDY' named entity 'NSP2' named entity 'RNA BINDING MOTIFS' named entity 'CELL LINES' named entity 'AEDES' named entity 'RNA INTERFERENCE' named entity 'GFP' named entity 'MODELS' named entity 'NSP3' named entity 'MAMMALIAN CELL' named entity 'BASED' named entity 'PROTEINS'

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